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The map label for this gene is gadA [H]

Identifier: 187734880

GI number: 187734880

Start: 443237

End: 444637

Strand: Reverse

Name: gadA [H]

Synonym: Amuc_0372

Alternate gene names: 187734880

Gene position: 444637-443237 (Counterclockwise)

Preceding gene: 187734881

Following gene: 187734879

Centisome position: 16.69

GC content: 52.32

Gene sequence:

>1401_bases
ATGTTTGATCCAAATCAAAAACCCGATACAGCCCGGGCGGAAAATTCCATTTTCGGTTCTCCGGAAGCAGATAACCTTCT
CCCCAAGGATGAGTTCCCGGATTATGCAATGCGCGCGGAGGACGCTTTCCAATTGGTGTCCGATGAATTGATGCTGGACG
GCAATGCCCGCCAGAATCTTGCAACCTTCTGCCAGACCTGGGATGAAAAGCAGGTCAGGATGCTGATGAATCTCAGCATT
AACAAGAACATGATTGACAAGGATGAATATCCCCAGACAGCCGCTATTGAGATGCGTTGCGCTGCCATTATTGCCAATCT
TTGGAATGCCAGCCAGAATGAAAAGCCTATTGGTTGTTCCACTATCGGTTCCAGTGAAGCATGCATGCTTGGAGGCATGG
CCGCCCTGTGGCGCTGGAGAGCCCGCCGGAAAGCCGCCGGCAAACCCATTGACAAGCCCAACCTGGTATGCGGCCCTGTC
CAAATCTGCTGGCACAAGTTCTGCCGCTATTGGGATATTGAAATGCGTGAAATCCCGATGGTGCCCGGAAAATGGAAGAT
GGATGTTGACCGGATGCTGGAACAGATTGATGAGAACACCATCGTTGTGGTTCCCACGTTCGGAGTGACCTATACGGGTG
CTTATGAATTGCCGGCGGAGATTGCCAAGGCCCTGGATGATTATGAAAACAAGACCGGCATCAGCGTTGACATCCATGTA
GATGGAGCCAGCGGCGGTTTCCTTGCTCCGTTCTGCGCCCCGGATCTTGTCTTTGACTTCCGCATTCCCCGCGTCAAGTC
CATCAGTGCTTCCGGCCATAAATACGGATTGGCTCCGCTCGGAGTGGGCTGGGTCGTTTGGAGAGATATTTCCGAACTGC
CTGAAGGGTTGATCTTCAATGTCAACTATCTTGGCGGGGAAATTCCCAATTTTGCCATTAACTTCTCACGACCGGCCGGA
CAGATTATCTGCCAGTATTATGATTTCATCCGACTGGGCAAGGAAGGCTATACGAACATCCATACACAGGCCTACGAAGT
AGCGAAGTACATTTCAGATGAGCTTTCCAAACTGGGGCCGTATGAATTCATTTGTACGGGCGATCCGGAAAAAGGTATTC
CCGCCGTCTGCTTCTTCATCAAGGATGGAGCCCGTGCGAACTACACGCTCTTTGACCTTTCGGACAAGCTGAGAACCCGC
GGTTGGCAGGTGCCCGCCTTCACGCTCCCTGCCAACTGTCAGGATACGGTGGTGATGCGCGTCATGGTTCGCCAGGGCTT
CTCCAAAGATTTGGCAGACCTGTTCCTGGAAGACTACAAGCGCATGCTCTCTTTCTTCGAGAAACACCCGGTCTCTTCTC
CGATGACGGCGGAAGAAGGAACGGCTTTCCACCATTCCTGA

Upstream 100 bases:

>100_bases
ACAATGGAAATATGAACGCTTTTATTCTCGGGTGCTTCTATCCTTCATGCGGGTTGCCTTTCACGGTTGGCCCGGAAACG
ATGAAACAATAGATACCGTC

Downstream 100 bases:

>100_bases
TGGAAGAAGCTCTGCTGAGCGATTTTTGTTGGATGAATAAGTGTGTGTAAGCGCAGCTCCGCAGTTGTCAAGGTTTGTGC
GTGGACCTGACGGCTGCGGA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQLVSDELMLDGNARQNLATFCQTWDEKQVRMLMNLSI
NKNMIDKDEYPQTAAIEMRCAAIIANLWNASQNEKPIGCSTIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPV
QICWHKFCRYWDIEMREIPMVPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELPAEIAKALDDYENKTGISVDIHV
DGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYGLAPLGVGWVVWRDISELPEGLIFNVNYLGGEIPNFAINFSRPAG
QIICQYYDFIRLGKEGYTNIHTQAYEVAKYISDELSKLGPYEFICTGDPEKGIPAVCFFIKDGARANYTLFDLSDKLRTR
GWQVPAFTLPANCQDTVVMRVMVRQGFSKDLADLFLEDYKRMLSFFEKHPVSSPMTAEEGTAFHHS

Sequences:

>Translated_466_residues
MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQLVSDELMLDGNARQNLATFCQTWDEKQVRMLMNLSI
NKNMIDKDEYPQTAAIEMRCAAIIANLWNASQNEKPIGCSTIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPV
QICWHKFCRYWDIEMREIPMVPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELPAEIAKALDDYENKTGISVDIHV
DGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYGLAPLGVGWVVWRDISELPEGLIFNVNYLGGEIPNFAINFSRPAG
QIICQYYDFIRLGKEGYTNIHTQAYEVAKYISDELSKLGPYEFICTGDPEKGIPAVCFFIKDGARANYTLFDLSDKLRTR
GWQVPAFTLPANCQDTVVMRVMVRQGFSKDLADLFLEDYKRMLSFFEKHPVSSPMTAEEGTAFHHS
>Mature_466_residues
MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQLVSDELMLDGNARQNLATFCQTWDEKQVRMLMNLSI
NKNMIDKDEYPQTAAIEMRCAAIIANLWNASQNEKPIGCSTIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPV
QICWHKFCRYWDIEMREIPMVPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELPAEIAKALDDYENKTGISVDIHV
DGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYGLAPLGVGWVVWRDISELPEGLIFNVNYLGGEIPNFAINFSRPAG
QIICQYYDFIRLGKEGYTNIHTQAYEVAKYISDELSKLGPYEFICTGDPEKGIPAVCFFIKDGARANYTLFDLSDKLRTR
GWQVPAFTLPANCQDTVVMRVMVRQGFSKDLADLFLEDYKRMLSFFEKHPVSSPMTAEEGTAFHHS

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=375, Percent_Identity=25.3333333333333, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1787769, Length=454, Percent_Identity=62.7753303964758, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1789934, Length=454, Percent_Identity=62.7753303964758, Blast_Score=617, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI17557272, Length=375, Percent_Identity=25.3333333333333, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543922, Length=338, Percent_Identity=26.3313609467456, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25148342, Length=407, Percent_Identity=22.8501228501229, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=461, Percent_Identity=37.0932754880694, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320500, Length=347, Percent_Identity=23.9193083573487, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355963, Length=460, Percent_Identity=23.9130434782609, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654344, Length=460, Percent_Identity=23.9130434782609, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52458; Mature: 52458

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQLVSDELMLDGNARQNL
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHH
ATFCQTWDEKQVRMLMNLSINKNMIDKDEYPQTAAIEMRCAAIIANLWNASQNEKPIGCS
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPVQICWHKFCRYWDIEMREIPM
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCCCHHCCCC
VPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELPAEIAKALDDYENKTGISVDIHV
CCCCCCCCHHHHHHHHCCCCEEEEECCCCEECCCHHCHHHHHHHHHHHCCCCCCEEEEEE
DGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYGLAPLGVGWVVWRDISELPEGLIFN
ECCCCCEECCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
VNYLGGEIPNFAINFSRPAGQIICQYYDFIRLGKEGYTNIHTQAYEVAKYISDELSKLGP
EHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
YEFICTGDPEKGIPAVCFFIKDGARANYTLFDLSDKLRTRGWQVPAFTLPANCQDTVVMR
EEEEECCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHCCCCCCEEECCCCCHHHHHHH
VMVRQGFSKDLADLFLEDYKRMLSFFEKHPVSSPMTAEEGTAFHHS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQLVSDELMLDGNARQNL
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHH
ATFCQTWDEKQVRMLMNLSINKNMIDKDEYPQTAAIEMRCAAIIANLWNASQNEKPIGCS
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPVQICWHKFCRYWDIEMREIPM
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCCCHHCCCC
VPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELPAEIAKALDDYENKTGISVDIHV
CCCCCCCCHHHHHHHHCCCCEEEEECCCCEECCCHHCHHHHHHHHHHHCCCCCCEEEEEE
DGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYGLAPLGVGWVVWRDISELPEGLIFN
ECCCCCEECCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
VNYLGGEIPNFAINFSRPAGQIICQYYDFIRLGKEGYTNIHTQAYEVAKYISDELSKLGP
EHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
YEFICTGDPEKGIPAVCFFIKDGARANYTLFDLSDKLRTRGWQVPAFTLPANCQDTVVMR
EEEEECCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHCCCCCCEEECCCCCHHHHHHH
VMVRQGFSKDLADLFLEDYKRMLSFFEKHPVSSPMTAEEGTAFHHS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]