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The map label for this gene is ynfJ [C]

Identifier: 187734875

GI number: 187734875

Start: 433636

End: 435423

Strand: Reverse

Name: ynfJ [C]

Synonym: Amuc_0367

Alternate gene names: 187734875

Gene position: 435423-433636 (Counterclockwise)

Preceding gene: 187734876

Following gene: 187734874

Centisome position: 16.34

GC content: 60.51

Gene sequence:

>1788_bases
ATGAATTCTCCACTCCCCCAGTCCCAGCCGCCGGATTCCGTCTGGTACGGATGGATTTTAAAACTGTTCCATCATTGGAA
GGTGGGGGAAAGGCAGGTTACTTATGTATGGGCCGTTCTCGTCGGCATGGTGGGCTCCTGCATGTCTCTGTTTTTCGAGT
GGATGGTGGAAGTGATCCAGTGGATGCTCACCGGCATCTGGTCGGATTCCCGCGTAGGAACCTTTTCCCTGGTGGAGCCG
GAATGGCGCATGTTTATTCCGGTGGCGGGCGGCCTGGTGGCCGGCTGCATTCTGCTGTTCCTGAAGAAAAGGATGCCGGG
GCAGGCTATGGAATACATGGAGGCCATGTCCATCGGGAACGGGCTGATCAAGTTCCGGGCCTCCGCCCTGAAAGTTTTTT
CCGCGGCATGGAGCATCGCTTCCGGCGCGGCCATCGGCAAGGAAGGGCCTATCATCCAGTCTTCCGCCCTGATTGCGTCT
ACCGTGGGGCAGCGTCTGCACGTATCCATTCCGCGGCTCCGTCTGCTGGTCGGCTGCGGAGCGGCAGCCGGGTTCACGAC
GGCGTTTCATGCCCCTTTTTCCGGCTGCCTGTTCGTGAGTGAAATCATCATCGGTACCGTGAGCATGGATATTCTGGCCC
CCCTGCTCATTGCCTCCTGTACCGGGTTCGTGATGCTGCATCTGCTGGGGGATCCCTCCCCTTTGTACAGTTCCCCGTTT
GAAAGCTTTACTGTCTTTTCCCAGTCTCTCTGGTGCATCGCCCTGGGGGCGGCGGCGGCCGTGGCGGCCAGGGGATGGGT
CAGTTTGCTGGATGCGGCCGCCAGATATTTGAATGGCCGCCAGTCCCTTCTGCCGCTGCGTTTGGCGGCCGCCGGGCTGG
TCGTGGGGATTCTGGCCGTTTTTTATCCGGAAGTGGTGGGCAACGGTCAGGCTCTCATTACCGGGCTGGTCCATGAGGAT
TACGGCACGCGGGAGGCGCTCGTCCTGCTTCTGGTGAAGGTGTCTGCCGTGGCGGTGGTGTTCGGCTGCGGTACGGTGGG
CGGAGCCATGACGCCCACCCTGTACGTGGGGAGCATGGTGGGTTTTATCTTCAGTACGGTTCTGACGTCCCTGGGAATGG
AGGGCAACCATACGGTGGCATATGCGATGGTGGGGATGGCGTCCTTTTTCGCCGTAGCTGCCCAGGCCCCCCTGACGGCC
CTGGTGATGGTGGTGGAGTTTTCCATGAGCGGACAGATGATTTATCCCTTGCTGATCGGTGTCGTAAGCGCTTATGGCAC
GGGAAAACTGATTCGCGCGCGTTCCATGTATGCCGCCAGCCTGGCCGGCAGTCCGGCGTCCGTCGTCAACCTGCCCATGG
CGCAAGTCCATGTGCATGACCTGGCGCGGCACGTGGTGCCCCAGGTGGCCCCGGATGCTTCCCTGGATGATGTCTCCTCC
CTGATGCTGCGGAATCCCGGAGATCTGGTTTTTGTGGTGAATAAGGATGGAATTTATGAAGGGGCCATCTATCCGGAGGA
CTTTCTGGCGGTCCGCCGGCAGATGGAGGAGCGGAAGGGGGCGGTTCCGGCCGATGCCGGGTCCTTGGTTCGTACGGGGG
CTCCCGTGCTGGACGCCGGCACCCATCTGACGGATGCCTTGAAGCTGTTTGAACAAACCCATCTGCCCGCCTTTCCCGTG
GTCTGGAAGAATACCGGTCGGCTAGACGGCGTGCTGTACCGCAACGCGTTGTTCCAGGTTATCACGGAGATGATGAAACG
GGAGTCCGGCGGAGACGTGGGCATGTAA

Upstream 100 bases:

>100_bases
TCTGGCTCCGGAGGGGCATGGGGCTGGGCAGTTTTTTCTTCCGTATCGAACAGCCGGAGGTATTTTTTTGTCACAATGGA
ATAGTATTGCCTTCTTTTCT

Downstream 100 bases:

>100_bases
AACGGAACGAAACAAGGGATGTATATTACATTTTTGTAAAAATCTTTTGTTCCCCGTTCGTAATATCATCAACATTAATT
GCTGGGATGTTATCCATATT

Product: Chloride channel core

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MNSPLPQSQPPDSVWYGWILKLFHHWKVGERQVTYVWAVLVGMVGSCMSLFFEWMVEVIQWMLTGIWSDSRVGTFSLVEP
EWRMFIPVAGGLVAGCILLFLKKRMPGQAMEYMEAMSIGNGLIKFRASALKVFSAAWSIASGAAIGKEGPIIQSSALIAS
TVGQRLHVSIPRLRLLVGCGAAAGFTTAFHAPFSGCLFVSEIIIGTVSMDILAPLLIASCTGFVMLHLLGDPSPLYSSPF
ESFTVFSQSLWCIALGAAAAVAARGWVSLLDAAARYLNGRQSLLPLRLAAAGLVVGILAVFYPEVVGNGQALITGLVHED
YGTREALVLLLVKVSAVAVVFGCGTVGGAMTPTLYVGSMVGFIFSTVLTSLGMEGNHTVAYAMVGMASFFAVAAQAPLTA
LVMVVEFSMSGQMIYPLLIGVVSAYGTGKLIRARSMYAASLAGSPASVVNLPMAQVHVHDLARHVVPQVAPDASLDDVSS
LMLRNPGDLVFVVNKDGIYEGAIYPEDFLAVRRQMEERKGAVPADAGSLVRTGAPVLDAGTHLTDALKLFEQTHLPAFPV
VWKNTGRLDGVLYRNALFQVITEMMKRESGGDVGM

Sequences:

>Translated_595_residues
MNSPLPQSQPPDSVWYGWILKLFHHWKVGERQVTYVWAVLVGMVGSCMSLFFEWMVEVIQWMLTGIWSDSRVGTFSLVEP
EWRMFIPVAGGLVAGCILLFLKKRMPGQAMEYMEAMSIGNGLIKFRASALKVFSAAWSIASGAAIGKEGPIIQSSALIAS
TVGQRLHVSIPRLRLLVGCGAAAGFTTAFHAPFSGCLFVSEIIIGTVSMDILAPLLIASCTGFVMLHLLGDPSPLYSSPF
ESFTVFSQSLWCIALGAAAAVAARGWVSLLDAAARYLNGRQSLLPLRLAAAGLVVGILAVFYPEVVGNGQALITGLVHED
YGTREALVLLLVKVSAVAVVFGCGTVGGAMTPTLYVGSMVGFIFSTVLTSLGMEGNHTVAYAMVGMASFFAVAAQAPLTA
LVMVVEFSMSGQMIYPLLIGVVSAYGTGKLIRARSMYAASLAGSPASVVNLPMAQVHVHDLARHVVPQVAPDASLDDVSS
LMLRNPGDLVFVVNKDGIYEGAIYPEDFLAVRRQMEERKGAVPADAGSLVRTGAPVLDAGTHLTDALKLFEQTHLPAFPV
VWKNTGRLDGVLYRNALFQVITEMMKRESGGDVGM
>Mature_595_residues
MNSPLPQSQPPDSVWYGWILKLFHHWKVGERQVTYVWAVLVGMVGSCMSLFFEWMVEVIQWMLTGIWSDSRVGTFSLVEP
EWRMFIPVAGGLVAGCILLFLKKRMPGQAMEYMEAMSIGNGLIKFRASALKVFSAAWSIASGAAIGKEGPIIQSSALIAS
TVGQRLHVSIPRLRLLVGCGAAAGFTTAFHAPFSGCLFVSEIIIGTVSMDILAPLLIASCTGFVMLHLLGDPSPLYSSPF
ESFTVFSQSLWCIALGAAAAVAARGWVSLLDAAARYLNGRQSLLPLRLAAAGLVVGILAVFYPEVVGNGQALITGLVHED
YGTREALVLLLVKVSAVAVVFGCGTVGGAMTPTLYVGSMVGFIFSTVLTSLGMEGNHTVAYAMVGMASFFAVAAQAPLTA
LVMVVEFSMSGQMIYPLLIGVVSAYGTGKLIRARSMYAASLAGSPASVVNLPMAQVHVHDLARHVVPQVAPDASLDDVSS
LMLRNPGDLVFVVNKDGIYEGAIYPEDFLAVRRQMEERKGAVPADAGSLVRTGAPVLDAGTHLTDALKLFEQTHLPAFPV
VWKNTGRLDGVLYRNALFQVITEMMKRESGGDVGM

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=413, Percent_Identity=29.7820823244552, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 63503; Mature: 63503

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]