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The map label for this gene is anfA [H]

Identifier: 187734874

GI number: 187734874

Start: 431910

End: 433436

Strand: Reverse

Name: anfA [H]

Synonym: Amuc_0366

Alternate gene names: 187734874

Gene position: 433436-431910 (Counterclockwise)

Preceding gene: 187734875

Following gene: 187734873

Centisome position: 16.27

GC content: 56.91

Gene sequence:

>1527_bases
ATGAATGAAATAAACAGGTCACCGGATTTGGAGATGGTCGTGCTGAAAGAAATCAGCTCCGCCATTGTCAATGAACGCAA
CGGCACGGCTTTGCTCCGGCATATTCTGGACGTGCTTTACCGCCGCATGAAAATGCTGCGCGGCACGTTCACCATCCGCC
GCGGGAACGATTTGATGATTGAAGCTTCCCACGGCCTGGACGAGCAGGAAATGAAACGCGGCCATTACCATGTGGGGGAA
GGCATTACGGGGCATGTGGCGGAAACGGGCAGGCCCCATGTGATTGAGGATATTTCCCGGGATTCCAGGTTCCTGAACCG
TACCCGCACGCGCAAAAGCGGTGAAAAAGTAGCCTTCATCTGCGTTCCCATCATTCATTTGCAGCAGGTCATCGGCACGT
TGAGCATTGACCGTGCCGTGGACCGGGACACCGACCTGGAACGGGATCAGAAACTGCTGGAGATTGTAGGCAATATTGTT
GGGGATGCTCTGGCTGCCAGCCTCCAGGCGCATGAGGAACGTGCCGCCCTGGTCGCGGAAAATGAAAAACTGCGGGAGCT
TTTGACGACTAATCCGGGCGAATTGATCGGCAATTGCAGCACTATGCTCCAGGTGTATGAACAGATCCGCCAGGTGGCGC
CCAGCGATGCCACGGTGCTGATACGGGGAAGTTCCGGGACGGGGAAGGAACTGGTGGCGCGCGCCGTGGTGAATCTGAGC
GGCAGAAAGGATAAGCCGCTGGTCACGCTGAATTGTGCGGCTATGCCGGAGAACCTGCTGGAAAGCGAGCTGTTTGGTCA
TGAAAAGGGTTCTTTTACCGGGGCCACCAGCCGCCGCATCGGCCGGGCGGAGGCGGCGGACGGCGGCACCCTGTTTCTGG
ATGAAATCGGGGATTTAAGCCTCCAGATGCAGGTGAAGCTGCTGCGCTTTCTTCAGGAAAAGACCTTTTCCCGCGTGGGA
AGCAACAGGGAGCTTCATGCAGACGTGCGCTTCATCGCCGCCACCAGCCGCAATCTGGAGGAACTGATGGCGGCGAGCAA
GTTCCGGGAAGACTTGTACTACCGGTTGAACATTTTCCCCATCGTTATGCCGGACCTCTCCAAACGCAAGGGGGACGTCA
TGCTGCTGGCGGAACATTTTCTTTCCAAGTTCAACCTGAAATACGGAAAGGATATCAAACGGCTGTCCACCCCCGCCATC
AACATGCTCATGGCTTACCACTGGCCCGGCAACGTCCGGGAGCTGGAAAACTGCATGGAACGTGCCGTCATTACGGCGCA
GGACGACTGCATTTACGGTTACAACCTTCCCGCCTCCCTCCAGATGCCCAGCCATGACGTCCCTTATTCCCGTGACGGGG
AGGCTCCCGCCGATTTGCCGACCATGGTAGATTCCTTTGAACGGGAATTGATTGTAGCCGCATTGAAACGTTCTCCGGGC
AATATGTCCGCCGCGGCCCGGGAACTGGGCATTTCCCCGCGGGTACTCCATTATAAGATGCACAGGCTGGGCCTGCAGAA
ATCATGA

Upstream 100 bases:

>100_bases
TCCCCGTGCCGTTTTTTTTTGCCCGGCTGCATGGTGGTGGAAGAAAAAGTAGTTGGTATGGCGGGGGCGTGCGGTTAATA
TTCGCCCAGCCCCGGATTGC

Downstream 100 bases:

>100_bases
AAGGCCTTGACCATCTTTTATCGGCCGTCTCCCGGTCCTTTTATCTGAGCATGCGCTTCCTGCCCCGGGAAATGCGCCCC
GGCGTGGCCGTGGGCTATCT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNEINRSPDLEMVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRGNDLMIEASHGLDEQEMKRGHYHVGE
GITGHVAETGRPHVIEDISRDSRFLNRTRTRKSGEKVAFICVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIV
GDALAASLQAHEERAALVAENEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGKELVARAVVNLS
GRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAADGGTLFLDEIGDLSLQMQVKLLRFLQEKTFSRVG
SNRELHADVRFIAATSRNLEELMAASKFREDLYYRLNIFPIVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAI
NMLMAYHWPGNVRELENCMERAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPTMVDSFERELIVAALKRSPG
NMSAAARELGISPRVLHYKMHRLGLQKS

Sequences:

>Translated_508_residues
MNEINRSPDLEMVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRGNDLMIEASHGLDEQEMKRGHYHVGE
GITGHVAETGRPHVIEDISRDSRFLNRTRTRKSGEKVAFICVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIV
GDALAASLQAHEERAALVAENEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGKELVARAVVNLS
GRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAADGGTLFLDEIGDLSLQMQVKLLRFLQEKTFSRVG
SNRELHADVRFIAATSRNLEELMAASKFREDLYYRLNIFPIVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAI
NMLMAYHWPGNVRELENCMERAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPTMVDSFERELIVAALKRSPG
NMSAAARELGISPRVLHYKMHRLGLQKS
>Mature_508_residues
MNEINRSPDLEMVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRGNDLMIEASHGLDEQEMKRGHYHVGE
GITGHVAETGRPHVIEDISRDSRFLNRTRTRKSGEKVAFICVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIV
GDALAASLQAHEERAALVAENEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGKELVARAVVNLS
GRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAADGGTLFLDEIGDLSLQMQVKLLRFLQEKTFSRVG
SNRELHADVRFIAATSRNLEELMAASKFREDLYYRLNIFPIVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAI
NMLMAYHWPGNVRELENCMERAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPTMVDSFERELIVAALKRSPG
NMSAAARELGISPRVLHYKMHRLGLQKS

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=472, Percent_Identity=39.8305084745763, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1790437, Length=308, Percent_Identity=51.2987012987013, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1788550, Length=316, Percent_Identity=43.6708860759494, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=45.7227138643068, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=45.7943925233645, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1788905, Length=342, Percent_Identity=43.2748538011696, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40.3125, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1790299, Length=348, Percent_Identity=39.367816091954, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=39.4321766561514, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=39.297124600639, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=45.4918032786885, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789828, Length=298, Percent_Identity=34.5637583892617, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87081858, Length=335, Percent_Identity=29.5522388059701, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56852; Mature: 56852

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEINRSPDLEMVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRGNDLMI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEE
EASHGLDEQEMKRGHYHVGEGITGHVAETGRPHVIEDISRDSRFLNRTRTRKSGEKVAFI
EECCCCCHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
CVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIVGDALAASLQAHEERAALVAE
EHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGKELVARAVVNLS
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCC
GRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAADGGTLFLDEIGDLS
CCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCH
LQMQVKLLRFLQEKTFSRVGSNRELHADVRFIAATSRNLEELMAASKFREDLYYRLNIFP
HHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEEE
IVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAINMLMAYHWPGNVRELENCME
EECCCCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCHHHHHHEEECCCCCHHHHHHHHH
RAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPTMVDSFERELIVAALKRSPG
HHEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NMSAAARELGISPRVLHYKMHRLGLQKS
CHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNEINRSPDLEMVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRGNDLMI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEE
EASHGLDEQEMKRGHYHVGEGITGHVAETGRPHVIEDISRDSRFLNRTRTRKSGEKVAFI
EECCCCCHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
CVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIVGDALAASLQAHEERAALVAE
EHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGKELVARAVVNLS
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCC
GRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAADGGTLFLDEIGDLS
CCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCH
LQMQVKLLRFLQEKTFSRVGSNRELHADVRFIAATSRNLEELMAASKFREDLYYRLNIFP
HHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEEE
IVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAINMLMAYHWPGNVRELENCME
EECCCCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCHHHHHHEEECCCCCHHHHHHHHH
RAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPTMVDSFERELIVAALKRSPG
HHEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NMSAAARELGISPRVLHYKMHRLGLQKS
CHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]