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The map label for this gene is tyrS [H]

Identifier: 187734653

GI number: 187734653

Start: 172287

End: 173468

Strand: Reverse

Name: tyrS [H]

Synonym: Amuc_0140

Alternate gene names: 187734653

Gene position: 173468-172287 (Counterclockwise)

Preceding gene: 187734655

Following gene: 187734652

Centisome position: 6.51

GC content: 57.19

Gene sequence:

>1182_bases
ATGACTATAGATGAGCAATTAGACATATTGATGGGCGGTACCGCCGTCGTGATCAGCCGCGAAGAGCTGAAGGAGCGTCT
CAAGCTGGGCCGCCCCCTGCGCGTGAAGCTGGGCGTGGACCCTACTGCGCCGGACATCCACCTGGGCCATACCGTGGCTA
TTGAGAAATTGCGCCAGTTCCAGGAACTTGGCCACCAGGCTGTTTTGCTCATCGGGGATTTCACCGCCACGATCGGCGAC
CCTTCCGGCCGTTCCGTGACCCGCCCCCCCCTTTCCCGTGAACAGGTGCTGGAGAATGCGGAGACATATACCAAGCAGGC
GTTCAAGATTCTGGACCGTGACAAGACGGAGATCGTGTATAATGGGGACTGGTTCCGCAAGATGACGTATGAGGAGGTGC
TGAAGCTTAATTCCCGCGTGACCATGCAGCAGATGCTGGCCCGGGAGGATTTCAAGGCCCGTGTGGAGGGAGGTAAGGAG
GTGCGCCTGCATGAGATGCAGTATCCGATTATGCAGGGCTGGGATTCCGTGGAAATCCGTGCGGACGTGGAACTGGGCGG
GACGGACCAGCTTTTCAACATCCTGGTGGGCCGCGACCTTCAGAAGGAGGAAGGCATGTTGCCGCAGATCGCCATGACGA
TGCCTCTTCTGGAAGGTCTGGACGGCGTTCGGAAGATGTCCAAGTCCTACGGGAATTACGTGGGCGTGGATGAGTCTCCG
GAGATGATGTTCGGCAAGATGATGAGCGCCAGCGACGAACTGATGGACCGTTATTACCTGGTGCTGCTGGGTGAGAAGCG
GGACATGGGATTGCATCCGATGGAAGCCAAAAAGCTCCTGGCCTGGAAAATCACGGCACGCTATCATGATTCCGCCGCTG
CGGATGCCGCGCGTTCTGACTGGGAAACCCGTTTTTCCAAGAGGGATTTGGCTGCCGCGGATTTGCCGGAAGTGGAGATT
GCCTCCCTGCCTGCCGACATGAATGCCCTGGCCCTGGTTTCCTTCCTGTTTGAGAATGTTTTCCAGGTGAAAAAATCCAA
TGGCGTTCTCCGCAAGGAGCATTTCACGCCCGGCGCTATCCAGTTGAATGATGTGAAAATGACAGACCCCTCCGCCGTTT
TGGAACTGGCTCCGGGCAGCATCCTGCGCCTGAGCAAGAAGCATGCTGTGCGTTTCAAATAG

Upstream 100 bases:

>100_bases
GGGACCGGTGGCGGGGGACTCTTGGCCGCGTTCGATGAGCCGCGCACAGGATATAGGCTTGAAAAGGCGCGGTGCCCCCG
GCACTCTATGCGCCAGATCA

Downstream 100 bases:

>100_bases
AACGCCGGAGGCGGCATTTACCTTTTTTCCTTCTCCATGCCGGGATTCCTTGCCAAATTGGATCGCTTCACCATCGGCCT
CATCGTCAGTGTGGGCGCGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAPDIHLGHTVAIEKLRQFQELGHQAVLLIGDFTATIGD
PSGRSVTRPPLSREQVLENAETYTKQAFKILDRDKTEIVYNGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKE
VRLHEMQYPIMQGWDSVEIRADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLEGLDGVRKMSKSYGNYVGVDESP
EMMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEAKKLLAWKITARYHDSAAADAARSDWETRFSKRDLAAADLPEVEI
ASLPADMNALALVSFLFENVFQVKKSNGVLRKEHFTPGAIQLNDVKMTDPSAVLELAPGSILRLSKKHAVRFK

Sequences:

>Translated_393_residues
MTIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAPDIHLGHTVAIEKLRQFQELGHQAVLLIGDFTATIGD
PSGRSVTRPPLSREQVLENAETYTKQAFKILDRDKTEIVYNGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKE
VRLHEMQYPIMQGWDSVEIRADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLEGLDGVRKMSKSYGNYVGVDESP
EMMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEAKKLLAWKITARYHDSAAADAARSDWETRFSKRDLAAADLPEVEI
ASLPADMNALALVSFLFENVFQVKKSNGVLRKEHFTPGAIQLNDVKMTDPSAVLELAPGSILRLSKKHAVRFK
>Mature_392_residues
TIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAPDIHLGHTVAIEKLRQFQELGHQAVLLIGDFTATIGDP
SGRSVTRPPLSREQVLENAETYTKQAFKILDRDKTEIVYNGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKEV
RLHEMQYPIMQGWDSVEIRADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLEGLDGVRKMSKSYGNYVGVDESPE
MMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEAKKLLAWKITARYHDSAAADAARSDWETRFSKRDLAAADLPEVEIA
SLPADMNALALVSFLFENVFQVKKSNGVLRKEHFTPGAIQLNDVKMTDPSAVLELAPGSILRLSKKHAVRFK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=398, Percent_Identity=25.6281407035176, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787925, Length=410, Percent_Identity=28.780487804878, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=212, Percent_Identity=32.0754716981132, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=257, Percent_Identity=29.1828793774319, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130383, Length=221, Percent_Identity=28.0542986425339, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44340; Mature: 44209

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAPDIHLGHTVAIEKLRQF
CCCCHHHHEEECCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHH
QELGHQAVLLIGDFTATIGDPSGRSVTRPPLSREQVLENAETYTKQAFKILDRDKTEIVY
HHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
NGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKEVRLHEMQYPIMQGWDSVEIR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCHHHCCCCCEEEE
ADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLEGLDGVRKMSKSYGNYVGVDESP
EEEECCCHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EMMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEAKKLLAWKITARYHDSAAADAARSD
HHHHHHHHCCHHHHHHEEEEEEEECCCCCCCCCHHHHHEEEEEEEEHCCCHHHHHHHHHH
WETRFSKRDLAAADLPEVEIASLPADMNALALVSFLFENVFQVKKSNGVLRKEHFTPGAI
HHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCE
QLNDVKMTDPSAVLELAPGSILRLSKKHAVRFK
EEECEEECCHHHHEEECCCHHEEECHHHCCCCC
>Mature Secondary Structure 
TIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAPDIHLGHTVAIEKLRQF
CCCHHHHEEECCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHH
QELGHQAVLLIGDFTATIGDPSGRSVTRPPLSREQVLENAETYTKQAFKILDRDKTEIVY
HHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
NGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKEVRLHEMQYPIMQGWDSVEIR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCHHHCCCCCEEEE
ADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLEGLDGVRKMSKSYGNYVGVDESP
EEEECCCHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EMMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEAKKLLAWKITARYHDSAAADAARSD
HHHHHHHHCCHHHHHHEEEEEEEECCCCCCCCCHHHHHEEEEEEEEHCCCHHHHHHHHHH
WETRFSKRDLAAADLPEVEIASLPADMNALALVSFLFENVFQVKKSNGVLRKEHFTPGAI
HHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCE
QLNDVKMTDPSAVLELAPGSILRLSKKHAVRFK
EEECEEECCHHHHEEECCCHHEEECHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA