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The map label for this gene is leuO

Identifier: 187734241

GI number: 187734241

Start: 75337

End: 76281

Strand: Direct

Name: leuO

Synonym: SbBS512_E0069

Alternate gene names: 187734241

Gene position: 75337-76281 (Clockwise)

Preceding gene: 187732583

Following gene: 187734215

Centisome position: 1.63

GC content: 45.5

Gene sequence:

>945_bases
ATGCCAGAGGTACAAACAGATCATTCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATT
AACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACCCGTGCCGCTCATGTTCTGGGTATGTCGCAACCTGCGGTCA
GTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCT
CGCGCATTTCAACTTTTTGGTTCAGTTCGCCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACC
CGCGAGTAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACA
TTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAATCAGAACACTGAACATCAGCTGCGTTATCAG
GAAACGGAGTTTGTGATTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTGCCATTATTTAAAGATGAAATGGT
GCTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGG
TTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGC
ATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTT
CGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCATGGCATGAAG
CTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAACAATTAGTCTCAATTTGCAAACGCTAA

Upstream 100 bases:

>100_bases
CTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAA
GTGTGACAGTGGAGTTAAGT

Downstream 100 bases:

>100_bases
CTGAATGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTG
TGGGCTCTCTATTTTAGGAT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLLLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR

Sequences:

>Translated_314_residues
MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLLLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR
>Mature_313_residues
PEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTAR
AFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQE
TEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQGM
AMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLLLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=314, Percent_Identity=99.3630573248408, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI87081766, Length=277, Percent_Identity=25.9927797833935, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786820, Length=286, Percent_Identity=24.4755244755245, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=295, Percent_Identity=23.0508474576271, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35701; Mature: 35570

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARL
CCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCS
HHEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVP
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEECCEEEEEHHHCCCCCCCCCC
LFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
CCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLLLKQNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCH
HQWMEEQLVSICKR
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARL
CCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCS
HHEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVP
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEECCEEEEEHHHCCCCCCCCCC
LFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
CCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLLLKQNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCH
HQWMEEQLVSICKR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]