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The map label for this gene is setA

Identifier: 187732583

GI number: 187732583

Start: 68685

End: 69767

Strand: Direct

Name: setA

Synonym: SbBS512_E0064

Alternate gene names: 187732583

Gene position: 68685-69767 (Clockwise)

Preceding gene: 187732393

Following gene: 187734241

Centisome position: 1.49

GC content: 48.11

Gene sequence:

>1083_bases
ATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGT
GGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGACTCT
TTTATACGGTGAATACTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGA
AAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCCATTGTTTGCATTTAATCGTCATTATCTGACGCTTAT
CACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATA
ACTCGGCGCGAGAAGTGGTGATGTTTTCGATTGCCGCCGGGATATTCACACTCAGTCTGGTATTGATTGCATTTATGCTT
CCGTCTGTGGCGCGGGTAGAACTGCCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGAT
GTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGT
TAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGC
TACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGAT
TTTCTTTCATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGA
TGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTA
ATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTC
TGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGA

Upstream 100 bases:

>100_bases
TTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCG
CCGCGCTAAAAAGGGAACGT

Downstream 100 bases:

>100_bases
TGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCAT
AAGCGGCAATGGCGTCGTCG

Product: sugar efflux transporter A

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNAPLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAG
YYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGV
ILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV

Sequences:

>Translated_360_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNAPLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAG
YYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGV
ILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
>Mature_360_residues
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRR
KLIIFCCLMAIGNAPLFAFNRHYLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAG
YYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGV
ILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=392, Percent_Identity=91.0714285714286, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1788495, Length=385, Percent_Identity=46.7532467532467, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1790091, Length=388, Percent_Identity=41.4948453608247, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 39237; Mature: 39237

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.7 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.7 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNTI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCEEEHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRHYLTLITCGVLLASLANTA
HHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCEECCCCC
QDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAG
CCCCEEEEEEHHHHHHHCCEEEEEECCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
YYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDL
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPGRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
CCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWIGLFYTVNTI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCEEEHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRHYLTLITCGVLLASLANTA
HHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCEECCCCC
QDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAG
CCCCEEEEEEHHHHHHHCCEEEEEECCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
YYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDL
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPGRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
CCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]