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The map label for this gene is ymfK [C]

Identifier: 187733300

GI number: 187733300

Start: 1147174

End: 1147830

Strand: Reverse

Name: ymfK [C]

Synonym: SbBS512_E1249

Alternate gene names: 187733300

Gene position: 1147830-1147174 (Counterclockwise)

Preceding gene: 187730275

Following gene: 187730851

Centisome position: 24.87

GC content: 43.38

Gene sequence:

>657_bases
ATGAAGTCTTTAGGTGAACGTCTCATCAACGCACGGCAAAAAGCTGGGTTAACACAAGATGCGTTGGCTAAAAAAGCTGG
GATCACCAGAGTTGCAATCAGTAAAGCCGAGCAAGGCCTTACAAAAAGTTTCAACGGTGACACCCTTTTTAAAGTTGCAG
CTGCACTGGGGTGTTCACCGCAGTGGCTTCAGAACGGAGATGAAAAAGATAAGCATTGGGAAAATAATGTTAAGAGCTGC
CCACAGAGAGACACAGCACACTCTTACCCTGTAATTAACTGGGTTCAGGCAGGATTATTCGCAACTTCTGGTGATGACTA
CAACATGTATGATCAGGATAATTGGAGGCATTCTGTAAAATACGCTGGTGAGAGGGGGTTCTGGCTGGAAGTGCACGGAG
ACTCAATGACTTCGCCCGTAGGAATAACATTTCCTGAAGGAATGTCGATCCTTGTCAACCCAGATAAAGAAGTTTTTTCA
GGGTGTTACGTCATCGCCAGAAAAAAATCCACCAATGAAGCAACATTCAAAAAATATATTTCTGACATGGGAAAGGCGTT
TCTAAAGCCCCTTAATCCACAATATCCAATCATAGAAATGGACAATGATTGCGAAATAGTAGGTGTTGTGGTTGATGCCA
GGTGGGATATTTTCTGA

Upstream 100 bases:

>100_bases
ACACCAGATACTTTGTTATTCAACGATGTTAACCGCGGGATTCCAAAGTATCAAGAAATTCTGTTACTTTCGTATCAACA
GCCATGAGAGGAGAAGAAAA

Downstream 100 bases:

>100_bases
CCAGACACAAAACACAAAAAGAAACCAAAGTATCAAAATCACTTGCCACACCTTGACACCTTAGTTACCATAAAACAAAG
TTCGTAACTGAGGTATCATC

Product: repressor protein C2

Products: NA

Alternate protein names: ORF-96 protein [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSFNGDTLFKVAAALGCSPQWLQNGDEKDKHWENNVKSC
PQRDTAHSYPVINWVQAGLFATSGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF

Sequences:

>Translated_218_residues
MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSFNGDTLFKVAAALGCSPQWLQNGDEKDKHWENNVKSC
PQRDTAHSYPVINWVQAGLFATSGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
>Mature_218_residues
MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSFNGDTLFKVAAALGCSPQWLQNGDEKDKHWENNVKSC
PQRDTAHSYPVINWVQAGLFATSGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF

Specific function: May control the expression of the integrase and inhibit excision of the mobile element R391, and regulate the expression of other genes as well [H]

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787391, Length=125, Percent_Identity=35.2, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF01381 HTH_3; PF00717 Peptidase_S24 [H]

EC number: NA

Molecular weight: Translated: 24361; Mature: 24361

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSFNGDTLFKVAAALGCSP
CCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
QWLQNGDEKDKHWENNVKSCPQRDTAHSYPVINWVQAGLFATSGDDYNMYDQDNWRHSVK
HHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEECCCCCCCCCCCCCHHHHE
YAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFSGCYVIARKKSTNEATFKKYI
ECCCCCEEEEECCCCCCCCCEEECCCCCEEEECCCHHHHCCEEEEEECCCCCHHHHHHHH
SDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
HHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSFNGDTLFKVAAALGCSP
CCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCH
QWLQNGDEKDKHWENNVKSCPQRDTAHSYPVINWVQAGLFATSGDDYNMYDQDNWRHSVK
HHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEECCCCCCCCCCCCCHHHHE
YAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFSGCYVIARKKSTNEATFKKYI
ECCCCCEEEEECCCCCCCCCEEECCCCCEEEECCCHHHHCCEEEEEECCCCCHHHHHHHH
SDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
HHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11157923; 12193633 [H]