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The map label for this gene is rfbB [C]

Identifier: 187732343

GI number: 187732343

Start: 1111744

End: 1112748

Strand: Reverse

Name: rfbB [C]

Synonym: SbBS512_E1204

Alternate gene names: 187732343

Gene position: 1112748-1111744 (Counterclockwise)

Preceding gene: 187730879

Following gene: 187731663

Centisome position: 24.11

GC content: 42.19

Gene sequence:

>1005_bases
ATGAAATTTCTGGTTACGGGAGCTGCTGGCTTTATCGGTTTCTATGTAAGTAAACGGCTTCTTGCAGCTGGTCATCAGGT
TGTAGGCATTGACAACTTAAATGATTATTACGATGTCAACCTCAAACAAGCACGACTTGATTTACTCAAGCACGACAATT
TCAGTTTTTATAAAATTGACCTGGCCGATCGCGAGAAAATGGCGGCACTGTTTGCAGACGAGCGGTTCGATCGCGTAATA
AACCTCGCTGCGCAAGCCGGTGTACGTTACTCCCTTGAAAATCCCAATGCATATGCAGATGCAAACCTGATTGGATTCCT
GAACATACTGGAAGGATGTCGCCATAATAATGTTCAGCATCTACTTTACGCTTCCTCCAGTTCTGTTTATGGCATGAACC
GCAAGATGCCTTTCTCTACAGATGACTCTGTAGATCATCCTGTTTCGCTTTATGCAGCAACTAAAAAAGCGAATGAACTC
ATGGCCCATACGTATTCTCATTTGTATGGCTTACCGACCACAGGGCTGCGTTTCTTTACGGTTTATGGTCCATGGGGACG
TCCGGATATGGCATTATTTAAATTTACTAAAGCCATGCTGGAAGGTAAAAGCATTGATGTTTACAACTTCGGCAAAATGA
AGCGTGACTTTACTTATATTGATGATATTGCCGAAGCTATTATTCGCTTACAGGATGTTATTCCAGAAAAAGATCCACAG
TGGACTGTAGAAACTGGATCGCCTGCAACAAGTTCAGCACCATATCGTGTCTATAACATTGGTAATAGTTCGCCTGTGGA
GTTGATGGACTATATCAATGCTTTAGAAGAGGCTCTGGGTATTGAAGCCAACAAGAATATGATGCCTCTCCAACCCGGTG
ATGTACTGGAAACCAGTGCTGATACAAAAGCACTGTATGACGTAATAGGATTCAAACCTGAAACGTCAGTTAAAGAGGGG
GTAAAGAACTTTGTAGAATGGTATCGTAACTTCTATAAAGTTTGA

Upstream 100 bases:

>100_bases
CTGATACCGATTATCTTTTTTTCTTGTCTAACGGTATGATTTGACTTTCAGTTATACCAGATGAAGATTGGCTATATTCT
AGCCTGAGCGAGGATTATAA

Downstream 100 bases:

>100_bases
TTTTACAAAACCATAAGAAAAGGCCCTAATATATTAGGGCCTTTTCTTAGAATAAAACAAAATAATTAATCATTGCCAAA
CAAGTCGCGCGTATAAACTT

Product: NAD dependent epimerase/dehydratase family

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQARLDLLKHDNFSFYKIDLADREKMAALFADERFDRVI
NLAAQAGVRYSLENPNAYADANLIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKDPQ
WTVETGSPATSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEG
VKNFVEWYRNFYKV

Sequences:

>Translated_334_residues
MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQARLDLLKHDNFSFYKIDLADREKMAALFADERFDRVI
NLAAQAGVRYSLENPNAYADANLIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKDPQ
WTVETGSPATSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEG
VKNFVEWYRNFYKV
>Mature_334_residues
MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQARLDLLKHDNFSFYKIDLADREKMAALFADERFDRVI
NLAAQAGVRYSLENPNAYADANLIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKDPQ
WTVETGSPATSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEG
VKNFVEWYRNFYKV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=24.9266862170088, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.9644970414201, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=23.0113636363636, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=23.0113636363636, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=23.0113636363636, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=27.0422535211268, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=26, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=23.2758620689655, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=24.0896358543417, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788589, Length=269, Percent_Identity=26.0223048327138, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=25.1412429378531, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=25, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=237, Percent_Identity=27.8481012658228, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=23.3727810650888, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=351, Percent_Identity=25.3561253561254, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=26.8011527377522, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=23.3236151603499, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37655; Mature: 37655

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQARLDLLKHDNFSFYKID
CEEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCCEEEEE
LADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADANLIGFLNILEGCRHNNVQH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEE
LLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPTTGLRFFT
EEEECCCCEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKDPQ
EECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WTVETGSPATSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSA
EEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQARLDLLKHDNFSFYKID
CEEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCCEEEEE
LADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADANLIGFLNILEGCRHNNVQH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEE
LLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPTTGLRFFT
EEEECCCCEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFTYIDDIAEAIIRLQDVIPEKDPQ
EECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WTVETGSPATSSAPYRVYNIGNSSPVELMDYINALEEALGIEANKNMMPLQPGDVLETSA
EEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]