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The map label for this gene is hypB

Identifier: 187732261

GI number: 187732261

Start: 2941130

End: 2942002

Strand: Reverse

Name: hypB

Synonym: SbBS512_E3150

Alternate gene names: 187732261

Gene position: 2942002-2941130 (Counterclockwise)

Preceding gene: 187731590

Following gene: 187730891

Centisome position: 63.73

GC content: 55.33

Gene sequence:

>873_bases
ATGTGTACAACATGCGGTTGCGGTGAAGGCAACCTGTATATCGAGGGTGATGAACATAACCCTCATTCCGCGTTTCGTAG
CGCGCCATTTGCCCCGGCGGCACGCCCGAAGATGAAAATCACCGGCATTAAAGCGCCTGAATTTACCCCCAGCCAGACTG
AAGAAGGCGACCTGCATTACGGTCATGGCGAAGCGGGCACTCACGCACCGGGCATGAGCCAGCGTCGGATGCTGGAAGTC
GAAATTGACGTGCTGGACAAAAACAACCGTCTGGCTGAACGCAACCGCGCGCGCTTTGCTGCCCGCAAGCAACTGGTGCT
CAACCTGGTTTCCAGCCCAGGTTCCGGTAAAACCACCCTGCTGACGGAAACCCTAATGCGCCTGAAAGACAGCGTTCCGT
GCGCGGTTATTGAAGGTGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTG
AACACAGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTAT
TCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTT
CCGTTACCGAAGGTGAAGACAAACCGCTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTT
GACCTGTTGCCGTATCTCAACTTTGACGTTGAGAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCAT
CCTTATTTCCGCCACCAGCGGTGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAG

Upstream 100 bases:

>100_bases
GCGCGTCCGCCGCTGTCCACAGTGTCATGGTGACATGCTACAGATTGTGGCAGACGACGGTTTACAGATTCGGCGGATAG
AAATAGACCAGGAGTGAGCG

Downstream 100 bases:

>100_bases
GCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCAAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTA
ACGTTAGTCGGCAGCTGCGA

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA

Sequences:

>Translated_290_residues
MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA
>Mature_290_residues
MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family

Homologues:

Organism=Escherichia coli, GI1789082, Length=290, Percent_Identity=100, Blast_Score=603, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPB_ECOL6 (P0AAN4)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755166.1
- ProteinModelPortal:   P0AAN4
- SMR:   P0AAN4
- EnsemblBacteria:   EBESCT00000043234
- GeneID:   1039092
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3287
- GeneTree:   EBGT00050000011813
- HOGENOM:   HBG364734
- OMA:   MFHAADV
- ProtClustDB:   PRK10463
- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202
- PIRSF:   PIRSF005624
- TIGRFAMs:   TIGR00073

Pfam domain/function: PF02492 cobW

EC number: NA

Molecular weight: Translated: 31565; Mature: 31565

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHY
CCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEE
GHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTL
CCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQVNTGKGCHLDAQMIADAAPRL
HHHHHHHHHCCCCEEEEECCHHHHCCHHEEEECCCCEEEEECCCCCEEHHHHHHCCCCCC
PLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA
HHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIKAPEFTPSQTEEGDLHY
CCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEE
GHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAARKQLVLNLVSSPGSGKTTL
CCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQVNTGKGCHLDAQMIADAAPRL
HHHHHHHHHCCCCEEEEECCHHHHCCHHEEEECCCCEEEEECCCCCEEHHHHHHCCCCCC
PLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DLLPYLNFDVEKCIACAREVNPEIEIILISATSGEGMDQWLNWLETQRCA
HHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157