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The map label for this gene is ascB

Identifier: 187731590

GI number: 187731590

Start: 2950417

End: 2951841

Strand: Reverse

Name: ascB

Synonym: SbBS512_E3160

Alternate gene names: 187731590

Gene position: 2951841-2950417 (Counterclockwise)

Preceding gene: 187732063

Following gene: 187732261

Centisome position: 63.95

GC content: 51.79

Gene sequence:

>1425_bases
ATGTCAGTATTTCCAGAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAATCTGAAGGTGCGTTCCGTGAAGGCGG
AAAAGGGCTGACCACCGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTC
AGTTGCGCGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAA
TCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCC
ACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTAC
GCCAGAACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTAACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGC
TGGTTGCCAGTGCGCTAGCCGCCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGC
AACTTCTACCCTTACAGCTGCAAACCGGAAGATGTCTGGGCGGCGCTGGAGAAAGATCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGCGGCGCGTATCCGGCTTACTCTGCCCGCGTATTTCGCGAAAAAGGGGTAACCATAGATAAAGCGCTGG
GCGATGATGAGATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAACCCGTATCTGCAAGTGAGCGACTGGGGCTGGGG
AATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACG
GCCTCGGCGCAAAAGATGAACTTGCTGCCAATGGCGAGATTAACGATGACTATCGCATCAGCTATTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGATGGCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGC
CTCTACGGGTGAAATGAGCAAACGCTACGGTTTTGTCTACGTAGACCGTGACGACGCAGGCAACGGCACGCTGACGCGCA
CACGTAAGAAATCGTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGGCTGCCTAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCAGGAAGGCCCGATAAGCGCAGCGC
ATCGGGCAATTTAGCATTTG

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALAAKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALAAKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMA
EMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALAAKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMN
ANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=30.2605210420842, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI32481206, Length=496, Percent_Identity=28.8306451612903, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI13273313, Length=491, Percent_Identity=27.2912423625255, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=26.7893660531697, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=24.2362525458248, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=98.3122362869198, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.6413502109705, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.673640167364, Blast_Score=506, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17552856, Length=485, Percent_Identity=30.1030927835052, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=492, Percent_Identity=29.8780487804878, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21356577, Length=499, Percent_Identity=25.0501002004008, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53786; Mature: 53655

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALAAKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKALGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDEITPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALAAKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKALGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]