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The map label for this gene is srlB

Identifier: 187732253

GI number: 187732253

Start: 2965096

End: 2965467

Strand: Reverse

Name: srlB

Synonym: SbBS512_E3173

Alternate gene names: 187732253

Gene position: 2965467-2965096 (Counterclockwise)

Preceding gene: 187732690

Following gene: 187731541

Centisome position: 64.24

GC content: 55.11

Gene sequence:

>372_bases
ATGACCGTTATTTATAAGACCACCATCACCCGTATCGGCGCGAGTGCCACTGACGCCCTCAGCGACCAGATGCTCATCAC
CTTTCGTGAAGGCGCGCCAGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGAAAGGTGCACTCCATC
CCGGTTTGCAATTTACGCTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGTGTGGCGGAAGACAACCTTCGCGAA
CTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTACC
CGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAA

Upstream 100 bases:

>100_bases
GGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCACCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTAT
CTATCAATAGAGGCTGAAAC

Downstream 100 bases:

>100_bases
AAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGTTGCCGAGGG
GTATCGCGTCGCGGTTGTCG

Product: PTS system glucitol/sorbitol-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRE
LGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFESVKE

Sequences:

>Translated_123_residues
MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRE
LGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFESVKE
>Mature_122_residues
TVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLREL
GHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFESVKE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=123, Percent_Identity=97.5609756097561, Blast_Score=249, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 13306; Mature: 13175

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTL
CEEEEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEE
GQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFES
CCCCCCEEEECCCCHHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEEECCC
VKE
CCC
>Mature Secondary Structure 
TVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTL
EEEEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEE
GQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFES
CCCCCCEEEECCCCHHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEEECCC
VKE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]