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The map label for this gene is yfiN [H]

Identifier: 187731886

GI number: 187731886

Start: 2794397

End: 2794816

Strand: Direct

Name: yfiN [H]

Synonym: SbBS512_E2993

Alternate gene names: 187731886

Gene position: 2794397-2794816 (Clockwise)

Preceding gene: 187731987

Following gene: 187730413

Centisome position: 60.54

GC content: 45.95

Gene sequence:

>420_bases
TTGATAAACAATTCCGATGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATATATCAATGATAC
CTGGGGTCATGCGACGGGCGATAGGGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAG
CATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAACAGATATGCTCA
GCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGTTACGC
GATGACCATTGAGCACGCCTCTGCGGAAAAATTACAAGAGCTTGCCGATCACAATATGTATCAGGCCAAACACCAGCGTG
CCGAAAAGCTGGTGAGATAG

Upstream 100 bases:

>100_bases
AGCGGAGCAGGAAGGACATCTGATGGAAATGGAAGCCACGCAAGCACCTTAAAATCACCATCATACACTAAATCAGTAAG
TTGGCAGCATTACCAATACG

Downstream 100 bases:

>100_bases
CAACAGATCCAAACCATGGCAGCCAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTA
TGGTGAAGACAGTTAAAGGC

Product: diguanylate cyclase (GGDEF) domain-containing protein, truncated

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MINNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICS
ALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR

Sequences:

>Translated_139_residues
MINNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICS
ALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR
>Mature_139_residues
MINNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICS
ALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=139, Percent_Identity=98.5611510791367, Blast_Score=291, Evalue=1e-80,
Organism=Escherichia coli, GI145693134, Length=123, Percent_Identity=34.9593495934959, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=34.9593495934959, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1787541, Length=132, Percent_Identity=35.6060606060606, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787262, Length=145, Percent_Identity=28.9655172413793, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1786584, Length=123, Percent_Identity=30.8943089430894, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 15757; Mature: 15757

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGD
CCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCC
EFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQE
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEECCCCHHHHHH
LADHNMYQAKHQRAEKLVR
HHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGD
CCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCC
EFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQE
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEECCCCHHHHHH
LADHNMYQAKHQRAEKLVR
HHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]