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The map label for this gene is insB1 [H]

Identifier: 187731646

GI number: 187731646

Start: 1288459

End: 1288953

Strand: Direct

Name: insB1 [H]

Synonym: SbBS512_E1409

Alternate gene names: 187731646

Gene position: 1288459-1288953 (Clockwise)

Preceding gene: 187733137

Following gene: 187731549

Centisome position: 27.91

GC content: 54.14

Gene sequence:

>495_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGAC
AACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCGGTGATGCTGCC
AACTTACTGATTTAG

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATAGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
TGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGG
TGCGTAACGGCAAAAGCACT

Product: IS1 transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVGDAA
NLLI

Sequences:

>Translated_164_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVGDAA
NLLI
>Mature_163_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVGDAAN
LLI

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=156, Percent_Identity=90.3846153846154, Blast_Score=296, Evalue=7e-82,
Organism=Escherichia coli, GI1788203, Length=156, Percent_Identity=90.3846153846154, Blast_Score=296, Evalue=7e-82,
Organism=Escherichia coli, GI1786203, Length=156, Percent_Identity=90.3846153846154, Blast_Score=296, Evalue=7e-82,
Organism=Escherichia coli, GI1787223, Length=156, Percent_Identity=88.4615384615385, Blast_Score=291, Evalue=2e-80,
Organism=Escherichia coli, GI1786470, Length=156, Percent_Identity=88.4615384615385, Blast_Score=289, Evalue=7e-80,
Organism=Escherichia coli, GI1786459, Length=156, Percent_Identity=88.4615384615385, Blast_Score=289, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19137; Mature: 19006

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVGDAANLLI
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCCHHHHHHCCHHHCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVGDAANLLI
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCCHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]