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The map label for this gene is rluB

Identifier: 187731466

GI number: 187731466

Start: 1363721

End: 1364593

Strand: Direct

Name: rluB

Synonym: SbBS512_E1493

Alternate gene names: 187731466

Gene position: 1363721-1364593 (Clockwise)

Preceding gene: 187734007

Following gene: 187730620

Centisome position: 29.54

GC content: 54.3

Gene sequence:

>873_bases
ATGAGCGAAAAATTACAGAAAGTGCTGGCCCGCGCTGGCCACGGCTCCCGCCGTGAAATCGAATCTATTATTGAAGCCGG
TCGTGTGAGTGTTGATGGCAAAATTGCTAAACTCGGCGATCGCGTTGAAGTTACCCCTGGGCTGAAAATTCGTATCGATG
GTCATCTGATTTCGGTACGTGAGTCCGCTGAACAAATTTGTCGCGTTCTGGCCTATTACAAACCGGAAGGTGAGTTGTGT
ACCCGTAACGATCCGGAAGGGCGTCCGACGGTGTTTGACCGTCTGCCAAAGCTGCGTGGCGCACGCTGGATTGCCGTAGG
TCGTCTGGACGTTAACACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCATCCAAGCC
GTGAAGTTGAACGTGAATACGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTG
CAACTGGAAGATGGTCCGGCAGCTTTTAAAATCATCAAGTTCAGCGGCGGCGAAGGGATCAACCAGTGGTACAACGTGAC
TCTGACCGAAGGCCGTAACCGTGAAGTTCGTCGTCTGTGGGAAGCGGTTGGTGTGCAGGTGAGCCGCCTGATCCGTGTTC
GTTACGGTGATATCCCACTGCCAAAAGGTCTGCCACGCGGTGGCTGGACGGAGCTGGACCTCGCCCAGACTAACTATCTG
CGCGAACTGGTGGAGCTTCCGCCAGAAACCAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCTAATCA
GATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGGCGTAATAACGGTTAA

Upstream 100 bases:

>100_bases
CAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTGTTGCACAAAAGCATATCAGACGCCTGGGAAGG
CGACACCAAAAGGAAGCTCT

Downstream 100 bases:

>100_bases
TCAAAGTATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCAATCCCTATTTGCGCCTTAACACCGGCATCAA
ACGCATGTTTGATGGGGCGC

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
QLEDGPAAFKIIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYL
RELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG

Sequences:

>Translated_290_residues
MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
QLEDGPAAFKIIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYL
RELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
>Mature_289_residues
SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCT
RNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQ
LEDGPAAFKIIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR
ELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=289, Percent_Identity=99.6539792387543, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790453, Length=293, Percent_Identity=30.0341296928328, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.6787564766839, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32610; Mature: 32479

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVR
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCEEEEECCEEEEHH
ESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKIIKFSGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHH
RELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVR
CHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCEEEEECCEEEEHH
ESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKIIKFSGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHH
RELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]