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The map label for this gene is yeaI [H]

Identifier: 187731228

GI number: 187731228

Start: 1854056

End: 1855531

Strand: Direct

Name: yeaI [H]

Synonym: SbBS512_E2033

Alternate gene names: 187731228

Gene position: 1854056-1855531 (Clockwise)

Preceding gene: 187730994

Following gene: 187732881

Centisome position: 40.17

GC content: 38.35

Gene sequence:

>1476_bases
ATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTGACGAAAATTGATCCTGGTCAAAA
CTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTAA
AAGCTCAAAGCTTTTTTATTTTCAGTTTATTGCTGACATTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACA
AATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTT
CATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTCTTAAGTGGCCTTATCT
ATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCT
ATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCACTCTTTTGTTATGGAAAAGACAACATCCT
TGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGTTGATTCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATC
TGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGACTACTGTCCGGACAACCATACTGCGACCAGGGGAATCAAC
TATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGTACACGATTAGCCAGCGAACTATG
GCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTACACCA
TCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAG
CTGCAACTCTCCAGCAAACTGGCAGTTCATGATGTGCTGACCAATATTTATAATCGGCGCTACTTTTTCAACAGCGTAGA
GTCATTATTGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAGTTCAAACGCATCAATGCCC
AATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAGCATCCGCCCTGATGATATT
TTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGATCG
TATACGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCA
CGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGC
GAGGGGGGCAACAAAGTGATTATTCATCATATTTAA

Upstream 100 bases:

>100_bases
TAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCA
GAAAACAATCATTCGGAAAA

Downstream 100 bases:

>100_bases
GTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCC
CAGCGGCGCAGAGATGAGTA

Product: diguanylate cyclase (GGDEF) domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI

Sequences:

>Translated_491_residues
MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI
>Mature_491_residues
MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT
NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGIN
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
LARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
EGGNKVIIHHI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1788085, Length=491, Percent_Identity=99.3890020366599, Blast_Score=988, Evalue=0.0,
Organism=Escherichia coli, GI1787262, Length=449, Percent_Identity=37.1937639198218, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=29.1208791208791, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082007, Length=227, Percent_Identity=29.9559471365639, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=234, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=28.9940828402367, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=29.2817679558011, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 56040; Mature: 56040

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFI
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
FSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
YGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGIN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQ
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCHH
AKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
HHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHH
EGGNKVIIHHI
CCCCEEEEEEC
>Mature Secondary Structure
MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFI
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
FSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
YGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGIN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQ
HHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCHH
AKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
HHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHH
EGGNKVIIHHI
CCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]