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The map label for this gene is emrB

Identifier: 187730572

GI number: 187730572

Start: 2978706

End: 2980253

Strand: Reverse

Name: emrB

Synonym: SbBS512_E3190

Alternate gene names: 187730572

Gene position: 2980253-2978706 (Counterclockwise)

Preceding gene: 187730269

Following gene: 187733432

Centisome position: 64.56

GC content: 56.27

Gene sequence:

>1548_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCGCAACTGGTCATTATGACGATTGCGCTGTCACTGGCGACATTCATGCAGGT
GCTGGACTCCACCATTGCTAACGTGGCGATCCCCACTATCGCCGGGAATCTGGGCTCATCGCTCAGCCAGGGAACGTGGG
TAATCACTTCTTTCGGGGTGGCGAATGCCATCTCGATCCCGCTTACCGGCTGGCTGGCAAAGCGCGTCGGGGAAGTGAAA
CTGTTCCTTTGGTCCACCATCGCCTTTGCTATTGCGTCGTGGGCGTGTGGTGTCTCCAGCAGCCTGAATATGCTGATCTT
CTTCCGTGTGATTCAGGGGATTGTCGCTGGGCCGTTGATCCCACTTTCGCAAAGTCTTTTGCTGAATAATTACCCGCCAG
CCAAACGCTCAATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTAT
ATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCAATTGGCGTGGCGGTGGTGTTGATGACACTGCA
AACCCTGCGCGGACGCGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCG
GTAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATTCTTACCGTGGTG
GCGGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT
GTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCGATTGTTCTGC
TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCG
GTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTA
TGTCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCC
AGGGGGTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCTGGTTTGCCACCGGAACGACTGGCGGCG
GCATCGAGCCTCTCTAACTTTACGCGAACGCTGGCGGGGTCTATCGGCACGTCGATAACCACGACCATGTGGACCAACCG
CGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGG
AAGGGCTTGGGATGACGCAACAGCAGGCATCAGGCTGGATTGCCCAGCAGATCACTAATCAGGGGCTGATTATTTCCGCC
AATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCCTTTGGCGC
AGGTGGCGGCGGAGGTGGTGCGCACTAA

Upstream 100 bases:

>100_bases
GTCGCGGTAAGTACCGCGCGTGAAATCAGCCTGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGCTAACGCTGG
CTAATCCAGAGGTGCGTGTG

Downstream 100 bases:

>100_bases
GTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAAATGTGCAGTTCCT
GCAACTTCTCTTTCGGCAGT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYVVCFYWRAYTFEPGMDFGASAWPQFIQGVAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Sequences:

>Translated_515_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYVVCFYWRAYTFEPGMDFGASAWPQFIQGVAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
>Mature_515_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYVVCFYWRAYTFEPGMDFGASAWPQFIQGVAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=99.009900990099, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=61.4624505928854, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=31.4553990610329, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=26.8691588785047, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=28.009828009828, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=536, Percent_Identity=22.3880597014925, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319770, Length=317, Percent_Identity=26.4984227129338, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55874; Mature: 55874

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHH
AVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQE
HHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYVVCFYWRAYTFEP
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMDFGASAWPQFIQGVAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
TTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEEC
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHH
AVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQE
HHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYVVCFYWRAYTFEP
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMDFGASAWPQFIQGVAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
TTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEEC
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]