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The map label for this gene is dos

Identifier: 187730158

GI number: 187730158

Start: 1587510

End: 1589933

Strand: Reverse

Name: dos

Synonym: SbBS512_E1752

Alternate gene names: 187730158

Gene position: 1589933-1587510 (Counterclockwise)

Preceding gene: 187733594

Following gene: 187733755

Centisome position: 34.44

GC content: 50.25

Gene sequence:

>2424_bases
ATGCGCCAGGATGCAGAGGTAATCATGAAGCTAACCGATGCGGAAAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGA
ACAAAATATGATGGGCGCGGTGTTAATTAACGAAAATGATGAAGTGATGTTTTTCAACCCCGCCGCAGAGAAGCTCTGGG
GGTACAAACGTGAAGAAGTCATTGGCAATAACATTGATATGCTGATTCCGCGGGATTTGCGTCCTGCACATCCTGAATAC
ATTCGTCATAACCGTGAAGGTGGTAAAGCGCGCGTCGAGGGGATGAGTCGGGAGCTGCAGCTGGAGAAAAAAGACGGCAG
TAAAATCTGGACCCGTTTTGCGCTATCGAAAGTGAGCGCCGAGGGGAAAGTTTATTACCTGGCGCTGGTACGGGATGCCA
GCGTAGAAATGGCGCAAAAAGAACAGACCCGACAATTGATTATTGCCGTTGACCATCTCGACCGACCGGTGATTGTCCTC
GATCCGGAACGCCATATTGTGCAGTGCAATCGCGCATTTACCGAAATGTTTGGTTACTGCATTAGCGAAGCCTGCGGTAT
GCAGCCCGATACACTCCTGAACATTCCTGAATTCCCTGCCGATAACCGCATTCGTTTACAACAGTTGCTATGGAAAACCG
CCCGCGATCAGGACGAATTTCTGCTGTTGACGCGCACCGGTGAAAAAATCTGGATTAAAGCCTCTATCAGCCCGGTTTAT
GACGTGCTCGCGCATCTGCAGAACCTGGTAATGACTTTCTCGGATATCACCGAAGAACGGCAAATCCGCCAGCTTGAAGG
CAATATTCTCGCCGCCATGTGCAGCAGCCCGCCATTTCATGAAATGGGGGAAATCATTTGTCGTAACATCGAATCTGTTC
TCAACGAATCGCATGTTTCGCTGTTCGCACTGCGCAACGGGATGCCGATACACTGGGCGTCATCTTCGCACGGTGCAGAA
GTTCAAAATGCGCAAAGCTGGTCAGCGACCATTCGTCAGCGTGATGGCGCGCCTGCGGGGATCCTGCAAATTAAAACCTC
GTCAGGAGCAGAAACCAGCGCCTTTATCGAACGCGTGGCAGATATCAGTCAGCATATGGCCGCGCTGGCGCTGGAACAGG
AAAAAAGCCGCCAGCATATTGAACAACTCATCCAATTTGATCCGATGACCGGTCTGCCAAATCGCAATAACCTGCACAAT
TACCTCGATGACCTGGTCGACAAAGCCGTCTCTCCGGTGGTGTATCTCATCGGTGTTGACCATATTCAGGATGTGATTGA
TAGCCTTGGCTATGCGTGGGCCGATCAGGCATTGCTGGAAGTGGTCAATCGCTTTCGTGGAAAACTCAAACCGGATCAGT
ATCTCTGTCGTATCGAAGGTACGCAGTTTGTCCTCGTGAGCCTGGAAAACGACGTCAGTAACATTACCCAGATCGCCGAT
GAGCTACGGAATGTGGTCAGCAAGCCGATAATGATTGACGATAAACCATTCCCGCTTACCTTGAGTATTGGCATCAGCTA
CGACGTGGGTAAAAACCGCGATTACCTACTCTCCACTGCTCACAATGCAATAGATTATATTCGCAAGAATGGCGGTAACG
GCTGGCAGTTCTTCAGCCCGGCGATGAACGAAATGGTAAAAGAGCGTTTGGTTTTAGGCGCAGCGCTGAAAGAAGCGATT
AGCAATAACCAACTGAAACTTGTTTACCAGCCGCAAATCTTCGCAGAAACGGGTGAACTGTACGGCATCGAAGCCCTTGC
GCGCTGGCACGATCCCCAGCATGGTCATGTGCCCCCTTCACGGTTTATTCCTCTCGCAGAAGAGATTGGTGAAATCGAAA
ATATTGGGCGCTGGGTCATCGCGGAAGCTTGCCGTCAGTTAGCAGAATGGCGTAGCCAGAATATTCATATCCCGGCGCTT
TCCGTTAACTTGTCGGCACTGCACTTTCGCAGTAATCAACTGCCTAATCAGGTGTCTGATGCAATGCACGCCTGGGGTAT
TGACGGCCACCAGCTGACGGTAGAAATCACGGAAAGCATGATGATGGAACACGATACCGAAATCTTTAAGCGCATTCAGA
TCCTGCGTGATATGGGCGTGGGCTTATCGGTAGATGATTTTGGTACGGGCTTTTCCGGATTATCCCGCTTAGTCAGTCTT
CCGGTAACGGAAATCAAAATTGACAAAAGTTTTGTCGATCGTTGTCTGACCGAAAAACGCATCCTTGCCTTACTTGAAGC
CATTACCAGCATTGGGCAAAGCCTCAATTTAACCGTCGTGGCGGAAGGCGTCGAAACCAAAGAGCAATTTGAGATGCTAC
GCAAGATCCACTGTCGCGTTATTCAGGGATATTTCTTTTCCCGCCCCCTACCCGCCGAAGAAATTCCAGGCTGGATGAGC
AGCGTGTTACCGCTGAAAATCTGA

Upstream 100 bases:

>100_bases
TCCTGACTATGAGCGCCTCATTCAAATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTGAAC
TCTGGAAAGCCAGTCTTTAG

Downstream 100 bases:

>100_bases
CAAATTCCTCTCGCCCGCACTCGCGGGTTTTCATTTAACGTGACACCGTCACTTAAATACTGTGATTTCAGCTACGATTC
CGGGAGATTCCTTCCTTAAC

Product: cAMP phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEACGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAE
VQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI

Sequences:

>Translated_807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEACGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAE
VQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI
>Mature_807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEACGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAE
VQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=799, Percent_Identity=99.1239048811014, Blast_Score=1640, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=439, Percent_Identity=33.7129840546697, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI226510982, Length=416, Percent_Identity=31.9711538461538, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1790496, Length=247, Percent_Identity=36.8421052631579, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=34.8739495798319, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87081980, Length=261, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786507, Length=325, Percent_Identity=30.1538461538462, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=33.8775510204082, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787055, Length=275, Percent_Identity=30.9090909090909, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788502, Length=255, Percent_Identity=29.8039215686275, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788849, Length=462, Percent_Identity=25.5411255411255, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI87082096, Length=264, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 91132; Mature: 91132

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEV
CCCCHHHHHEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHH
IGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSA
CCCCCCEEECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCC
EGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYC
CCCEEEEEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
ISEACGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHH
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHE
LFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVA
EEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHH
HIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHH
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSP
HHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCH
AMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCCHH
RFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHEEEEECCCCCCHHHHH
AMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRV
CCHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IQGYFFSRPLPAEEIPGWMSSVLPLKI
HHHHHHCCCCCHHHCCCHHHHCCCCCC
>Mature Secondary Structure
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEV
CCCCHHHHHEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHH
IGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSA
CCCCCCEEECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCC
EGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYC
CCCEEEEEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
ISEACGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHH
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHE
LFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVA
EEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHH
HIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHH
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSP
HHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCH
AMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCCHH
RFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEHEEEEECCCCCCHHHHH
AMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRV
CCHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IQGYFFSRPLPAEEIPGWMSSVLPLKI
HHHHHHCCCCCHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]