BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

Click here to switch to the map view.

The map label for this gene is braG [H]

Identifier: 186477793

GI number: 186477793

Start: 3424594

End: 3425349

Strand: Reverse

Name: braG [H]

Synonym: Bphy_3048

Alternate gene names: 186477793

Gene position: 3425349-3424594 (Counterclockwise)

Preceding gene: 186477794

Following gene: 186477792

Centisome position: 98.45

GC content: 61.51

Gene sequence:

>756_bases
ATGAGCACAGTGAGCGAACAGGAAAACGCATCAGTCAGTGTCGTGAGCGGCGAGCCCGCGCTGGAGATCGCCGGATTGCA
GGCGTGGTACGGCGAGTCGCACATTCTGCATGGCGTCGATCTGACCGTGAGCCGAGGCGAAGTCGTCACGCTGCTGGGTC
GCAATGGCGCGGGCCGCACCACGACGCTGCGCGCGATCATGGGCTTGACGGGGCGCCGCACGGGCTCGATTCGCGTCGGC
GGACGCGAGACCATATCGATGCCGACGCATCGAATTGCCCATTGCGGCGTCGGCTATTGTCCGGAAGAACGCGGTATTTT
TTCGAGCCTGTCGTGCGAAGAGAATCTGCTGCTGCCGCCGCCCGTCGGCGACAAGTCGTTGATGATGTCGATCGACGAGA
TTTACCAGATGTTTCCCAATCTCCAGGAACGGCGCATGAGCCAGGGCACGCGGCTGTCAGGCGGCGAGCAGCAGATGCTG
GCCGTCGCGCGCATCCTGAGAACGGGTGCGAACCTGCTGTTGCTCGATGAAATCTCGGAAGGTCTCGCGCCTGTGATCGT
GCAGGCCCTCGCGCGCATGATCGTGACGCTGAAAGCCCGCGGCTACACGATCGTGATGGTCGAACAGAACTTCCGCTTTG
CCGCGCCGCTAGCGGATCGTTTCTATGTGATGGAGCACGGACGCATCGTGGAGCATTTCGGTGCAAAGGAACTCGAAAGC
AAAATGCCGGTGCTGCACGATTTGCTCGGGGTATGA

Upstream 100 bases:

>100_bases
AAGTGCTCGCCGAAGGCACGTACGCGGAAGTCTCGAAGAACCCGCTCGTCATGCAGGCCTATATGGGCAGTGCGGACGCA
GCGCTTGCCGGAGCGCATGC

Downstream 100 bases:

>100_bases
GCCGGGGAGTGCGTACCTGATTGCCTCTGACAACCACAACGCAGAACGAAGGAGACAGTAATGAAAAGGAAGACACTCGC
GCGGTTCGCATCCATTTGCT

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSTVSEQENASVSVVSGEPALEIAGLQAWYGESHILHGVDLTVSRGEVVTLLGRNGAGRTTTLRAIMGLTGRRTGSIRVG
GRETISMPTHRIAHCGVGYCPEERGIFSSLSCEENLLLPPPVGDKSLMMSIDEIYQMFPNLQERRMSQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALARMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAKELES
KMPVLHDLLGV

Sequences:

>Translated_251_residues
MSTVSEQENASVSVVSGEPALEIAGLQAWYGESHILHGVDLTVSRGEVVTLLGRNGAGRTTTLRAIMGLTGRRTGSIRVG
GRETISMPTHRIAHCGVGYCPEERGIFSSLSCEENLLLPPPVGDKSLMMSIDEIYQMFPNLQERRMSQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALARMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAKELES
KMPVLHDLLGV
>Mature_250_residues
STVSEQENASVSVVSGEPALEIAGLQAWYGESHILHGVDLTVSRGEVVTLLGRNGAGRTTTLRAIMGLTGRRTGSIRVGG
RETISMPTHRIAHCGVGYCPEERGIFSSLSCEENLLLPPPVGDKSLMMSIDEIYQMFPNLQERRMSQGTRLSGGEQQMLA
VARILRTGANLLLLDEISEGLAPVIVQALARMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAKELESK
MPVLHDLLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=194, Percent_Identity=29.3814432989691, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI31657092, Length=231, Percent_Identity=25.5411255411255, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI27881501, Length=208, Percent_Identity=29.8076923076923, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI30795238, Length=208, Percent_Identity=29.8076923076923, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI21536376, Length=198, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI47078218, Length=196, Percent_Identity=29.5918367346939, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45446740, Length=196, Percent_Identity=29.5918367346939, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=29.5336787564767, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI153792144, Length=198, Percent_Identity=26.7676767676768, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4505771, Length=235, Percent_Identity=26.8085106382979, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI9961252, Length=235, Percent_Identity=26.8085106382979, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27262624, Length=219, Percent_Identity=26.4840182648402, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI27262626, Length=219, Percent_Identity=26.4840182648402, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI150417984, Length=197, Percent_Identity=27.9187817258883, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI27477115, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI42741659, Length=236, Percent_Identity=26.271186440678, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI148612844, Length=222, Percent_Identity=28.8288288288288, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI255708477, Length=222, Percent_Identity=28.8288288288288, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87082268, Length=211, Percent_Identity=41.2322274881517, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1789593, Length=216, Percent_Identity=31.9444444444444, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=31.1965811965812, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=28.8888888888889, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787029, Length=218, Percent_Identity=27.5229357798165, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=30.5936073059361, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787792, Length=215, Percent_Identity=30.6976744186047, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787089, Length=224, Percent_Identity=29.4642857142857, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788761, Length=221, Percent_Identity=28.9592760180996, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=25.4980079681275, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=29.9539170506912, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787370, Length=233, Percent_Identity=27.0386266094421, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790190, Length=220, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48995001, Length=223, Percent_Identity=29.5964125560538, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786253, Length=231, Percent_Identity=30.7359307359307, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790162, Length=245, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081791, Length=217, Percent_Identity=28.110599078341, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790525, Length=218, Percent_Identity=26.1467889908257, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787105, Length=224, Percent_Identity=25.4464285714286, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789991, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788472, Length=229, Percent_Identity=26.2008733624454, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=28.0701754385965, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786872, Length=225, Percent_Identity=28, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786398, Length=222, Percent_Identity=24.3243243243243, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787712, Length=219, Percent_Identity=27.8538812785388, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790467, Length=208, Percent_Identity=27.8846153846154, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081709, Length=220, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081782, Length=245, Percent_Identity=24.4897959183673, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787112, Length=238, Percent_Identity=28.1512605042017, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788210, Length=225, Percent_Identity=26.2222222222222, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI48994943, Length=238, Percent_Identity=25.2100840336134, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788644, Length=243, Percent_Identity=28.3950617283951, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790544, Length=249, Percent_Identity=24.0963855421687, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786563, Length=223, Percent_Identity=25.5605381165919, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789586, Length=228, Percent_Identity=25.4385964912281, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788165, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=232, Percent_Identity=29.7413793103448, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=210, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=210, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=231, Percent_Identity=26.8398268398268, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=210, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=231, Percent_Identity=26.8398268398268, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=231, Percent_Identity=26.8398268398268, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=213, Percent_Identity=24.4131455399061, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=211, Percent_Identity=27.4881516587678, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71984940, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=213, Percent_Identity=24.8826291079812, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=202, Percent_Identity=26.2376237623762, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115535101, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996550, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648314, Length=226, Percent_Identity=27.8761061946903, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24666092, Length=235, Percent_Identity=27.2340425531915, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI221512771, Length=217, Percent_Identity=27.1889400921659, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28574150, Length=234, Percent_Identity=23.5042735042735, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007328, Length=187, Percent_Identity=26.2032085561497, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573571, Length=218, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28573573, Length=218, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24650853, Length=205, Percent_Identity=25.8536585365854, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85725262, Length=191, Percent_Identity=28.7958115183246, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24650855, Length=205, Percent_Identity=25.8536585365854, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17136662, Length=214, Percent_Identity=26.6355140186916, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI116007184, Length=236, Percent_Identity=23.3050847457627, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27401; Mature: 27270

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVSEQENASVSVVSGEPALEIAGLQAWYGESHILHGVDLTVSRGEVVTLLGRNGAGRT
CCCCCCCCCCEEEEECCCCCEEEECCHHHCCCCEEEECEEEEECCCCEEEEEECCCCCCH
TTLRAIMGLTGRRTGSIRVGGRETISMPTHRIAHCGVGYCPEERGIFSSLSCEENLLLPP
HHHHHHHCCCCCCCCEEEECCCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECCC
PVGDKSLMMSIDEIYQMFPNLQERRMSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALARMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAKELES
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHEEEECCCCHHHHCCHHHHHH
KMPVLHDLLGV
CCHHHHHHHCC
>Mature Secondary Structure 
STVSEQENASVSVVSGEPALEIAGLQAWYGESHILHGVDLTVSRGEVVTLLGRNGAGRT
CCCCCCCCCEEEEECCCCCEEEECCHHHCCCCEEEECEEEEECCCCEEEEEECCCCCCH
TTLRAIMGLTGRRTGSIRVGGRETISMPTHRIAHCGVGYCPEERGIFSSLSCEENLLLPP
HHHHHHHCCCCCCCCEEEECCCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECCC
PVGDKSLMMSIDEIYQMFPNLQERRMSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALARMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAKELES
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHEEEECCCCHHHHCCHHHHHH
KMPVLHDLLGV
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]