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The map label for this gene is ilvG [H]

Identifier: 186477762

GI number: 186477762

Start: 3383745

End: 3385445

Strand: Direct

Name: ilvG [H]

Synonym: Bphy_3016

Alternate gene names: 186477762

Gene position: 3383745-3385445 (Clockwise)

Preceding gene: 186477761

Following gene: 186477765

Centisome position: 97.26

GC content: 63.73

Gene sequence:

>1701_bases
ATGTCGCTGTCTTCCGATGCTTCAAATATGACCACGGGCGCACGGCTCATGGTCGACGCGCTGCTGACGCATGGCGTCGA
GCGCGTGTTTTGCGTGCCTGGCGAAAGTTTTCTGGCGATCCTCGATTCGTTGCACGACGAGACGTCGCAGATTCAGACAG
TTGTGTGCCGCCACGAAGCGGCCGCCGCCAACATGGCGGAAGCCGTCGGCAAGCTGACGGGCCGGCCGGGCATCGCGATC
GTCACGCGCGGACCGGGCGCGACGCACGCATCGATCGGCGTGCACACGGCGTTTCAGGACTCGACGCCGATGATCCTGCT
GATCGGCCAATGTGCGCGCGAGCATCTGGACCGCGAGGCGTTTCAGGAAATCGACTACCGGCGCATGTTCGGCCAGATGG
CGAAGTGGGTCGCGCAGATTGACGACGCGCGCCGCATTCCCGAGTACATGAGCCACGCGTTCCACGTCGCGACGTCGGGT
CGCCCTGGCCCTGTCGTACTGTCGTTGCCTGAAGACATGTTGAGCGACGCGTGTGCCGCGATGCCGGGTGCGCCCGCTTA
TCAGCGCGTGGCGGCCGCGCCGGCGCCGCAGCAGATCGAGCGTCTGCGCGGCCTGCTCGAAGGCGCGAAGAAACCGATGG
TGATCGCGGGCGGCAGCGGCTGGACGCCGCAAGCGTGCGAAGACCTCCGCACCTTCATCGAGAACTGGCAGCTACCCATC
GGCCTCGCGTTCCGCTTCCAGGACACGCTCGACAACGAGCATCCGAACTATGCGGGCGATGTGGGTCTCGGCATCAATCC
CGCGCTTGCCAAACGCATCCAGGACGCCGACGTGCTGCTCGCGCTCGGCCCGCGCCTGGGCGAAGCGACGACGAATGGCT
ACACGCTGCTCGACATTCCGAAGACGAAGCAGACATTGATCCACGTGCATCAAGGCGCGGACGAGCTGGGCCGCGTGTAT
GCCGCCGATCTGCCCATCGTGTCGGGCATGCCGGAAATCGCATCGATGCTGGCGGCGCTGAAACGGGTGGCACAACCCGC
CTGGTCAGGCAGCGCGAAGGAAGCGCACGACGCGTACCTCGAATGGCGCAAGCCGCGCATGGTTCCGGGCGATGTGCAAC
TCGGCGAGATCATGCTGCAGCTTCGCGAACATCTGCCGAAGGACGCGATTCTCACGAACGGCGCGGGCAATTACGCGACA
TGGCTGCATCGTCATTTTGCGTATCGCCATTTCCGTTCCCAGTTGGCACCGACTAGCGGCGCGATGGGTTATGGCGTGCC
GGCGGCGATTGCGGCCAAGTCGATGTATCCGGACCGGACGGTGGTCGCGTTCGCAGGCGATGGCTGTTTCATGATGTCGT
CATCGGAGATCGCGACGGCGGTGCAATACGACTTGCCGGTGATATTCATCGTCGTGAACAACAGCCAGTACGGCACGATC
CGGATGCACCAGGAGCGCCATTATCCGAATCGCGTGCATGGAACGGGGCTGACCAATCCAGACTTCGCGGCGTTTGCAAG
ATCATTCGGCGCCCACGGCGAAACGGTGGAACGCACAGAAGACTTCATGCCCGCATTCAAGCGCGCGCAAGAGTCGAAGA
AACCGGCGGTAATCGAAGTCCGGATGATCCAGGAAGCGAGCACACCGGGCGCGACACTGGAGCAGGTGCGCGAGCAGGGC
AGGAAACTGCGCGGCGAATAA

Upstream 100 bases:

>100_bases
TCGCGGTAAACATCATCGAATGTTGACGCTTTTGCGAATGACATTGAAGAGGGTCTCGGCGTAAGCTGTCGAAGCTCTTC
TGACTGGAGGTGTGTTCGCA

Downstream 100 bases:

>100_bases
GCAGGAGCCACGGGCGCCTTTTGGGGCCCGTGGGCACGAGTCAAGAATTGGCGGTGTGAGCTGCTTTCTTTTGCCTACTT
TTCTTTGCAGTCGGCAAAGA

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSLSSDASNMTTGARLMVDALLTHGVERVFCVPGESFLAILDSLHDETSQIQTVVCRHEAAAANMAEAVGKLTGRPGIAI
VTRGPGATHASIGVHTAFQDSTPMILLIGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDARRIPEYMSHAFHVATSG
RPGPVVLSLPEDMLSDACAAMPGAPAYQRVAAAPAPQQIERLRGLLEGAKKPMVIAGGSGWTPQACEDLRTFIENWQLPI
GLAFRFQDTLDNEHPNYAGDVGLGINPALAKRIQDADVLLALGPRLGEATTNGYTLLDIPKTKQTLIHVHQGADELGRVY
AADLPIVSGMPEIASMLAALKRVAQPAWSGSAKEAHDAYLEWRKPRMVPGDVQLGEIMLQLREHLPKDAILTNGAGNYAT
WLHRHFAYRHFRSQLAPTSGAMGYGVPAAIAAKSMYPDRTVVAFAGDGCFMMSSSEIATAVQYDLPVIFIVVNNSQYGTI
RMHQERHYPNRVHGTGLTNPDFAAFARSFGAHGETVERTEDFMPAFKRAQESKKPAVIEVRMIQEASTPGATLEQVREQG
RKLRGE

Sequences:

>Translated_566_residues
MSLSSDASNMTTGARLMVDALLTHGVERVFCVPGESFLAILDSLHDETSQIQTVVCRHEAAAANMAEAVGKLTGRPGIAI
VTRGPGATHASIGVHTAFQDSTPMILLIGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDARRIPEYMSHAFHVATSG
RPGPVVLSLPEDMLSDACAAMPGAPAYQRVAAAPAPQQIERLRGLLEGAKKPMVIAGGSGWTPQACEDLRTFIENWQLPI
GLAFRFQDTLDNEHPNYAGDVGLGINPALAKRIQDADVLLALGPRLGEATTNGYTLLDIPKTKQTLIHVHQGADELGRVY
AADLPIVSGMPEIASMLAALKRVAQPAWSGSAKEAHDAYLEWRKPRMVPGDVQLGEIMLQLREHLPKDAILTNGAGNYAT
WLHRHFAYRHFRSQLAPTSGAMGYGVPAAIAAKSMYPDRTVVAFAGDGCFMMSSSEIATAVQYDLPVIFIVVNNSQYGTI
RMHQERHYPNRVHGTGLTNPDFAAFARSFGAHGETVERTEDFMPAFKRAQESKKPAVIEVRMIQEASTPGATLEQVREQG
RKLRGE
>Mature_565_residues
SLSSDASNMTTGARLMVDALLTHGVERVFCVPGESFLAILDSLHDETSQIQTVVCRHEAAAANMAEAVGKLTGRPGIAIV
TRGPGATHASIGVHTAFQDSTPMILLIGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDARRIPEYMSHAFHVATSGR
PGPVVLSLPEDMLSDACAAMPGAPAYQRVAAAPAPQQIERLRGLLEGAKKPMVIAGGSGWTPQACEDLRTFIENWQLPIG
LAFRFQDTLDNEHPNYAGDVGLGINPALAKRIQDADVLLALGPRLGEATTNGYTLLDIPKTKQTLIHVHQGADELGRVYA
ADLPIVSGMPEIASMLAALKRVAQPAWSGSAKEAHDAYLEWRKPRMVPGDVQLGEIMLQLREHLPKDAILTNGAGNYATW
LHRHFAYRHFRSQLAPTSGAMGYGVPAAIAAKSMYPDRTVVAFAGDGCFMMSSSEIATAVQYDLPVIFIVVNNSQYGTIR
MHQERHYPNRVHGTGLTNPDFAAFARSFGAHGETVERTEDFMPAFKRAQESKKPAVIEVRMIQEASTPGATLEQVREQGR
KLRGE

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=584, Percent_Identity=22.9452054794521, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI21361361, Length=564, Percent_Identity=25.531914893617, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=29.6296296296296, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=29.3577981651376, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI1786717, Length=577, Percent_Identity=26.1698440207972, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788716, Length=570, Percent_Identity=26.6666666666667, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=25.8467023172906, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=564, Percent_Identity=25.177304964539, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=516, Percent_Identity=26.5503875968992, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=516, Percent_Identity=26.5503875968992, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=26.6199649737303, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=22.9129662522202, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=560, Percent_Identity=22.1428571428571, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=558, Percent_Identity=27.2401433691756, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61649; Mature: 61518

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSSDASNMTTGARLMVDALLTHGVERVFCVPGESFLAILDSLHDETSQIQTVVCRHEA
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHH
AAANMAEAVGKLTGRPGIAIVTRGPGATHASIGVHTAFQDSTPMILLIGQCAREHLDREA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHH
FQEIDYRRMFGQMAKWVAQIDDARRIPEYMSHAFHVATSGRPGPVVLSLPEDMLSDACAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCHHHHHHHHHH
MPGAPAYQRVAAAPAPQQIERLRGLLEGAKKPMVIAGGSGWTPQACEDLRTFIENWQLPI
CCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCE
GLAFRFQDTLDNEHPNYAGDVGLGINPALAKRIQDADVLLALGPRLGEATTNGYTLLDIP
EEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECC
KTKQTLIHVHQGADELGRVYAADLPIVSGMPEIASMLAALKRVAQPAWSGSAKEAHDAYL
CCHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EWRKPRMVPGDVQLGEIMLQLREHLPKDAILTNGAGNYATWLHRHFAYRHFRSQLAPTSG
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
AMGYGVPAAIAAKSMYPDRTVVAFAGDGCFMMSSSEIATAVQYDLPVIFIVVNNSQYGTI
CCCCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCHHHHHEEECCCEEEEEEECCCCCEE
RMHQERHYPNRVHGTGLTNPDFAAFARSFGAHGETVERTEDFMPAFKRAQESKKPAVIEV
EEEHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
RMIQEASTPGATLEQVREQGRKLRGE
EEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLSSDASNMTTGARLMVDALLTHGVERVFCVPGESFLAILDSLHDETSQIQTVVCRHEA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHH
AAANMAEAVGKLTGRPGIAIVTRGPGATHASIGVHTAFQDSTPMILLIGQCAREHLDREA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHH
FQEIDYRRMFGQMAKWVAQIDDARRIPEYMSHAFHVATSGRPGPVVLSLPEDMLSDACAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCHHHHHHHHHH
MPGAPAYQRVAAAPAPQQIERLRGLLEGAKKPMVIAGGSGWTPQACEDLRTFIENWQLPI
CCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCE
GLAFRFQDTLDNEHPNYAGDVGLGINPALAKRIQDADVLLALGPRLGEATTNGYTLLDIP
EEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECC
KTKQTLIHVHQGADELGRVYAADLPIVSGMPEIASMLAALKRVAQPAWSGSAKEAHDAYL
CCHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EWRKPRMVPGDVQLGEIMLQLREHLPKDAILTNGAGNYATWLHRHFAYRHFRSQLAPTSG
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
AMGYGVPAAIAAKSMYPDRTVVAFAGDGCFMMSSSEIATAVQYDLPVIFIVVNNSQYGTI
CCCCCCHHHHHHHHCCCCCEEEEEECCCEEEECCCHHHHHEEECCCEEEEEEECCCCCEE
RMHQERHYPNRVHGTGLTNPDFAAFARSFGAHGETVERTEDFMPAFKRAQESKKPAVIEV
EEEHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
RMIQEASTPGATLEQVREQGRKLRGE
EEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]