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The map label for this gene is rep [H]

Identifier: 186477746

GI number: 186477746

Start: 3362935

End: 3365130

Strand: Reverse

Name: rep [H]

Synonym: Bphy_3000

Alternate gene names: 186477746

Gene position: 3365130-3362935 (Counterclockwise)

Preceding gene: 186477752

Following gene: 186477744

Centisome position: 96.72

GC content: 62.93

Gene sequence:

>2196_bases
GTGCCGTGCAACCCGCCCGCCGCGCCGCCTGGCACTCGCCGTTTCGCTGCATGTTCTCGGGCCGTTCTGCGCGAATCGCG
CCCTCAATCGATTACTCATCCACACACGATGTCCGCAGGCCTGAATTCCGCCCAAAGCGAAGCGGTGCGCTATCTCGACG
GTCCCTGCCTCGTGCTCGCGGGCGCGGGCAGCGGCAAGACGCGCGTGATCACGCAGAAGATCGCGCACCTGATCGAAAAC
AAAGGCTTCGAGCCGCGCCATATCGCCGCCGTCACCTTCACGAACAAGGCCGCAGCGGAAATGCGCGAGCGCGTCGGCAA
GCTCCTCGAGGGCAAGACGCTCACCGCGCCCGGCAAGGAAGGACGCAAGGTGCCCGTCAACCAGTTGACGGTGTGCACGT
TCCACTCGCTCGGCGTGCAGATTTTGCGTCAGGAGGCGGAACACGTCGGCCTCAAGCCGCAGTTCTCGATCATGGATTCC
GACGACTGCTTCGGCATGATCCAGGAGCAGGTCGGCTCGACGGACAAAGGCTTCATCCGCAAGATCCAGACGATCATCTC
GTTGTGGAAGAACGGCCTCGTCATGCCGGACGAGGCGATGAGGATCGCGTCGAACGAGGACGAGCACCAGGCGGCGATTG
TCTATCGAAACTACGTCGCGACGCTGCACGCGTATCAGGCCGTCGATTTCGACGATCTGATCCGCCTGCCCGCCGAACTC
TTCAAGCAGAACGAGCAGGTGCGCGACCGCTGGCAGAACAAGCTGCGCTATCTGCTGATCGACGAGTATCAGGACACCAA
CGCGTGCCAGTACGAACTGGTGAAGCTGCTGGCGGGCACGCGCGCTGCGTTCACGGCGGTGGGCGACGACGATCAGGCGA
TCTACGGCTGGCGCGGCGCGACGCTCGAAAATCTGGCGCAGCTCAGCAAGGACTTTCCGAGGCTGCACGTCATCAAGCTC
GAGCAGAATTACCGCTCGACGGTGCGCATCCTGACGGCCGCGAACAACGTCATCGCGAACAATCCAAAGCTGTTCGAGAA
GAAGCTGTGGTCCGAGCACGGCATGGGCGATTCGATCACTGTCACCGGCTGCAACGACGAAGAGCATGAGGCGGAGTCCG
TCGTGTTCCGGCTGTCGGCGCACAAGTTCGAGCGGCGCGCGCAGTTCCGCGACTACGCGATCCTGTATCGCGGCAATTTC
CAGGCGCGTATTTTCGAACAGGTGCTGCGGCGCGAGCGGATTCCGTATGTGCTGTCGGGCGGCCAGTCTTTCTTCGACAA
GGCCGAGATCAAGGACATCTGCGCATACCTGCGGCTGATCGCGAACGCGGACGACGATCCCGCGTTTATCCGCGCGATCA
CGACGCCGCGCCGAGGCGTCGGCAATACGACGCTCGAAGCGCTCGGCGCGTTTGCGGGACAGGCCAAGGTATCGCTGTTC
GAGGCGGTGTACATGGGCGGTATCGAGGCGCGGCTGTCGGCGCGTCAGGTCGAGCCGCTGCGCGTATTCTGCGATTTCAT
GCAGCGTCTTCGGGATCGTGCGGACAAGGACGCGGCTACCTCCGTGCTGAACGACATGATGGAGGCGATTCACTACGAAG
CCTATCTGTACGACGCATTCGACGAGCGTCAAGCTCAGGCGAAATGGCAAAACGTGCTGGAGTTTCTGGAGTGGCTCAAG
CGCAAGGGCACGAAGCCCGAGCCGGAAGCAGGCGCCGAACAGACGGGCTACGACACCGCCGACGGTCTCGCCGATACTGG
CAAGAACCTGCTCGGACTGATTCAGACGGTCGCGCTGATGTCGATGCTCGAAGGCAAGAATGAAGACCCGGATGCCGTGC
GGCTGTCGACGGTGCACGCGTCGAAGGGACTCGAATATCCGCACGTGTTTCTGGTCGGCGTCGAGGAAGGCATCATGCCG
CATCGCGGGGGGGCGGAAGACGATGGTCCGATCGACGACGAGCGCATCGAGGAAGAGCGCCGCCTGATGTACGTCGCTAT
CACGCGTGCGCAGCGCAGCCTGCACCTGAACTGGTGCAAGAAGCGCAAGCGGGCACGCGAGACCGTGGTGTGCGAGCCGT
CGCGCTTTATCCCCGAAATGGGGCTCGACGAAGCACCGCCTCCGACGCCGGAAGAAGCGCCGATGTCGCCGAAGGACCGG
CTCGCGAGTCTCAAGGCGCTGTTGCAGAAGGCATAA

Upstream 100 bases:

>100_bases
GGCATCGGAAAGGGCATTCGTGTTAACATCGCGGCTCTCACGCGAACGGCAAAAAGCGCATGGAATCAATTGCGTGGCGC
GTCGGCGGAGGTTTCGGCAA

Downstream 100 bases:

>100_bases
GAAAAAATCCCCGCACAGGGCGGGGATTGTTGTGTGCCGGAACTATCGTCGCTGCTTATTTCGCTGCATTGACCATGTAG
TCGACGGCGGCCTTTACGTC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MPCNPPAAPPGTRRFAACSRAVLRESRPQSITHPHTMSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEN
KGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTAPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDS
DDCFGMIQEQVGSTDKGFIRKIQTIISLWKNGLVMPDEAMRIASNEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAEL
FKQNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLLAGTRAAFTAVGDDDQAIYGWRGATLENLAQLSKDFPRLHVIKL
EQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDSITVTGCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNF
QARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANADDDPAFIRAITTPRRGVGNTTLEALGAFAGQAKVSLF
EAVYMGGIEARLSARQVEPLRVFCDFMQRLRDRADKDAATSVLNDMMEAIHYEAYLYDAFDERQAQAKWQNVLEFLEWLK
RKGTKPEPEAGAEQTGYDTADGLADTGKNLLGLIQTVALMSMLEGKNEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMP
HRGGAEDDGPIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDR
LASLKALLQKA

Sequences:

>Translated_731_residues
MPCNPPAAPPGTRRFAACSRAVLRESRPQSITHPHTMSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEN
KGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTAPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDS
DDCFGMIQEQVGSTDKGFIRKIQTIISLWKNGLVMPDEAMRIASNEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAEL
FKQNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLLAGTRAAFTAVGDDDQAIYGWRGATLENLAQLSKDFPRLHVIKL
EQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDSITVTGCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNF
QARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANADDDPAFIRAITTPRRGVGNTTLEALGAFAGQAKVSLF
EAVYMGGIEARLSARQVEPLRVFCDFMQRLRDRADKDAATSVLNDMMEAIHYEAYLYDAFDERQAQAKWQNVLEFLEWLK
RKGTKPEPEAGAEQTGYDTADGLADTGKNLLGLIQTVALMSMLEGKNEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMP
HRGGAEDDGPIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDR
LASLKALLQKA
>Mature_730_residues
PCNPPAAPPGTRRFAACSRAVLRESRPQSITHPHTMSAGLNSAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIENK
GFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTAPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSD
DCFGMIQEQVGSTDKGFIRKIQTIISLWKNGLVMPDEAMRIASNEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAELF
KQNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLLAGTRAAFTAVGDDDQAIYGWRGATLENLAQLSKDFPRLHVIKLE
QNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDSITVTGCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQ
ARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANADDDPAFIRAITTPRRGVGNTTLEALGAFAGQAKVSLFE
AVYMGGIEARLSARQVEPLRVFCDFMQRLRDRADKDAATSVLNDMMEAIHYEAYLYDAFDERQAQAKWQNVLEFLEWLKR
KGTKPEPEAGAEQTGYDTADGLADTGKNLLGLIQTVALMSMLEGKNEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPH
RGGAEDDGPIDDERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRL
ASLKALLQKA

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=700, Percent_Identity=46.5714285714286, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI2367296, Length=667, Percent_Identity=35.0824587706147, Blast_Score=353, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6322369, Length=728, Percent_Identity=27.8846153846154, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=659, Percent_Identity=23.3687405159332, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82037; Mature: 81906

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCNPPAAPPGTRRFAACSRAVLRESRPQSITHPHTMSAGLNSAQSEAVRYLDGPCLVLA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEE
GAGSGKTRVITQKIAHLIENKGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTAPGKE
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
GRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQVGSTDKGFIR
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHH
KIQTIISLWKNGLVMPDEAMRIASNEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAEL
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FKQNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLLAGTRAAFTAVGDDDQAIYGWRGA
HHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCEEEECCCC
TLENLAQLSKDFPRLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDSIT
CHHHHHHHHHCCCCEEEEEECCCCHHHEEHHHHCCCHHCCCCHHHHHHHHHHCCCCCEEE
VTGCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEC
GQSFFDKAEIKDICAYLRLIANADDDPAFIRAITTPRRGVGNTTLEALGAFAGQAKVSLF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHH
EAVYMGGIEARLSARQVEPLRVFCDFMQRLRDRADKDAATSVLNDMMEAIHYEAYLYDAF
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DERQAQAKWQNVLEFLEWLKRKGTKPEPEAGAEQTGYDTADGLADTGKNLLGLIQTVALM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMLEGKNEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGAEDDGPIDDERIEEER
HHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
RLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDR
HEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHH
LASLKALLQKA
HHHHHHHHHCC
>Mature Secondary Structure 
PCNPPAAPPGTRRFAACSRAVLRESRPQSITHPHTMSAGLNSAQSEAVRYLDGPCLVLA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEE
GAGSGKTRVITQKIAHLIENKGFEPRHIAAVTFTNKAAAEMRERVGKLLEGKTLTAPGKE
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
GRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQVGSTDKGFIR
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCHHHHH
KIQTIISLWKNGLVMPDEAMRIASNEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAEL
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FKQNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLLAGTRAAFTAVGDDDQAIYGWRGA
HHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCEEEECCCC
TLENLAQLSKDFPRLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGDSIT
CHHHHHHHHHCCCCEEEEEECCCCHHHEEHHHHCCCHHCCCCHHHHHHHHHHCCCCCEEE
VTGCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEC
GQSFFDKAEIKDICAYLRLIANADDDPAFIRAITTPRRGVGNTTLEALGAFAGQAKVSLF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHH
EAVYMGGIEARLSARQVEPLRVFCDFMQRLRDRADKDAATSVLNDMMEAIHYEAYLYDAF
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DERQAQAKWQNVLEFLEWLKRKGTKPEPEAGAEQTGYDTADGLADTGKNLLGLIQTVALM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMLEGKNEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGAEDDGPIDDERIEEER
HHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
RLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDR
HEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHH
LASLKALLQKA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]