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The map label for this gene is ttcA

Identifier: 186477640

GI number: 186477640

Start: 3247716

End: 3248726

Strand: Reverse

Name: ttcA

Synonym: Bphy_2892

Alternate gene names: 186477640

Gene position: 3248726-3247716 (Counterclockwise)

Preceding gene: 186477641

Following gene: 186477639

Centisome position: 93.38

GC content: 62.22

Gene sequence:

>1011_bases
ATGAACGCTCCCGACACCCTGACGGGCCTCGAAGCGAACGCGCCCGTTACCGAAGAAGCCCCCGCCGCCAGCGAGGCCGA
GCGCAAGCGCGCGCACACGCGTCGCGAGCAGAAAGAGCAGTACGAGAACAACAAGCTGTTCAAGCGCCTCGCGCGCCAGG
TCGGCCAGGCGATCGGCGACTTCAACATGATCGAGGATGGCGACAAGGTGATGGTGTGCCTGTCGGGCGGCAAGGACAGC
TACGCGATGCTGGATGTCCTGATGCGCCTGCGCGAGCGCGCGCCGATTCACTTCGACATCGTCGCCGTGAATCTCGACCA
GAAGCAGCCGGGCTTTCCGGAACACGTGCTGCCCGAGTATCTGAGCAAGCTCGACATTCCGTATCACATCGAGAATCAGG
ACACGTACAGCATCGTCAAGCGCCTCGTACCCGAGGGCAAGACAACGTGCTCGCTATGCTCGCGCCTGCGCCGCGGCATT
CTCTACCGCGTGGCGGGCGAGCTCGGCGCGACGAAGATCGCGCTCGGCCATCATCGCGACGACATCCTGCAGACGCTGCT
GCTGAACCTGTTCTACGGCGGCAAGCTGAAGGGCATGCCGCCCAAGCTGCAATCCGACGACGGCAAGAACGTCGTGATTC
GTCCGCTCGCGTATGTGAAGGAAACGGACCTCGAGAAGTACGCGGAACTGCGTCAATTCCCGATCATCCCGTGCAACCTG
TGCGGCAGCCAGCCGAACCTGAAACGCGCGGAAATGAAGGCGCTGATCCGCGAGTGGGACAAGCGCTTCCCGGGGCGCGT
CGACAACATGTTCAACGCATTGTCGAACGTGGTGCCGTCGCACCTGATGGATCACAAACTTTTTCCGTTCGCGAGTCTGC
GCGCGACGGGCGAAGCGGACCCGCATGGCGACATCGCTTTCGACGAAGAACCGTGTTCGGCCGATTCCGCGTCGAACCAG
ACGCAGCGGCCATCGCAAACGGTTTCGTTCGTTCAGTTTGACGACATCTGA

Upstream 100 bases:

>100_bases
GCAAGTGCGGGGCGTGCGTCTGTCGACGGAAAAGCCCGATGTCTATCCCGACTGCGACGCCGTGGGCGTCGAGGTCTTCC
AGATCAAAGAGGACTGAGCG

Downstream 100 bases:

>100_bases
CACCTTGCACTCCCCGCAAAGCCTTTGAAAACAGGCATTTGCGGGGCGATGCAGACACATCCAGCGTGCTAAAATCGCGC
ACTCCAAACTCGTCAAATAC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNAPDTLTGLEANAPVTEEAPAASEAERKRAHTRREQKEQYENNKLFKRLARQVGQAIGDFNMIEDGDKVMVCLSGGKDS
YAMLDVLMRLRERAPIHFDIVAVNLDQKQPGFPEHVLPEYLSKLDIPYHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGI
LYRVAGELGATKIALGHHRDDILQTLLLNLFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELRQFPIIPCNL
CGSQPNLKRAEMKALIREWDKRFPGRVDNMFNALSNVVPSHLMDHKLFPFASLRATGEADPHGDIAFDEEPCSADSASNQ
TQRPSQTVSFVQFDDI

Sequences:

>Translated_336_residues
MNAPDTLTGLEANAPVTEEAPAASEAERKRAHTRREQKEQYENNKLFKRLARQVGQAIGDFNMIEDGDKVMVCLSGGKDS
YAMLDVLMRLRERAPIHFDIVAVNLDQKQPGFPEHVLPEYLSKLDIPYHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGI
LYRVAGELGATKIALGHHRDDILQTLLLNLFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELRQFPIIPCNL
CGSQPNLKRAEMKALIREWDKRFPGRVDNMFNALSNVVPSHLMDHKLFPFASLRATGEADPHGDIAFDEEPCSADSASNQ
TQRPSQTVSFVQFDDI
>Mature_336_residues
MNAPDTLTGLEANAPVTEEAPAASEAERKRAHTRREQKEQYENNKLFKRLARQVGQAIGDFNMIEDGDKVMVCLSGGKDS
YAMLDVLMRLRERAPIHFDIVAVNLDQKQPGFPEHVLPEYLSKLDIPYHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGI
LYRVAGELGATKIALGHHRDDILQTLLLNLFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELRQFPIIPCNL
CGSQPNLKRAEMKALIREWDKRFPGRVDNMFNALSNVVPSHLMDHKLFPFASLRATGEADPHGDIAFDEEPCSADSASNQ
TQRPSQTVSFVQFDDI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=284, Percent_Identity=66.5492957746479, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI193211090, Length=289, Percent_Identity=31.4878892733564, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=240, Percent_Identity=28.75, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651972, Length=230, Percent_Identity=27.8260869565217, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_BURP8 (B2JIL8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001859110.1
- ProteinModelPortal:   B2JIL8
- SMR:   B2JIL8
- GeneID:   6244375
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2892
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 37819; Mature: 37819

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDTLTGLEANAPVTEEAPAASEAERKRAHTRREQKEQYENNKLFKRLARQVGQAIGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNMIEDGDKVMVCLSGGKDSYAMLDVLMRLRERAPIHFDIVAVNLDQKQPGFPEHVLPEY
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHH
LSKLDIPYHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRD
HHHCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
DILQTLLLNLFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELRQFPIIPCNL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHC
CGSQPNLKRAEMKALIREWDKRFPGRVDNMFNALSNVVPSHLMDHKLFPFASLRATGEAD
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
PHGDIAFDEEPCSADSASNQTQRPSQTVSFVQFDDI
CCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECCC
>Mature Secondary Structure
MNAPDTLTGLEANAPVTEEAPAASEAERKRAHTRREQKEQYENNKLFKRLARQVGQAIGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNMIEDGDKVMVCLSGGKDSYAMLDVLMRLRERAPIHFDIVAVNLDQKQPGFPEHVLPEY
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHH
LSKLDIPYHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRD
HHHCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
DILQTLLLNLFYGGKLKGMPPKLQSDDGKNVVIRPLAYVKETDLEKYAELRQFPIIPCNL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHC
CGSQPNLKRAEMKALIREWDKRFPGRVDNMFNALSNVVPSHLMDHKLFPFASLRATGEAD
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCC
PHGDIAFDEEPCSADSASNQTQRPSQTVSFVQFDDI
CCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA