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The map label for this gene is mlaA [H]

Identifier: 186477515

GI number: 186477515

Start: 3116772

End: 3117719

Strand: Reverse

Name: mlaA [H]

Synonym: Bphy_2767

Alternate gene names: 186477515

Gene position: 3117719-3116772 (Counterclockwise)

Preceding gene: 186477516

Following gene: 186477514

Centisome position: 89.61

GC content: 63.29

Gene sequence:

>948_bases
ATGATGACGACACGCTTACGTGTCGCGGGGATCGCGCTGGCTGTGGCAACGTTTGCCGGTTGTGCAACCGTTCAGAACCC
GACGAAAGAAGACCCCATCGAGGGCTTTAACCGTACGATCTTCACGTTCAACGACACCGTCGATAAATACGCGCTGAAGC
CCGTCGCGAAGGGCTACGTGTTTGTCACGCCGCAGCCGGTGCGCGATAGCGTGACGAACTTCTTTTCGAACATCGGCGAC
GTGTACATCGCCGCGAACAACCTGCTGCAACTGAAGATCGCCGACGGGGTGTCGGACATCATGCGGATCGTGATCAACAC
GATCTTCGGTGTCGGCGGACTGTTCGATGTCGCGACGATCGCGAAGCTGCCGAAGCACGACAACGACCTCGGTCTGACGC
TGGGTCACTATGGCGTGCCGCCGGGGCCGTATCTGGTGCTGCCACTGTTTGGGCCGAGCACGGTGCGCGACGGCGTCGGC
ATGGTCGGCAACTACTTCATCAACCCGCTCACGTATGTGCAGCCGGACAGCTTGAGCTGGGCGCTGTATGGCCTGAACGT
GGTCAACACGCGTGCGAACCTGCTGAGTGCGAGCGACGTGCTGGAAGGTGCAGCCCTCGACAAGTATTCGTTCGTGCGTA
ACGCATATCTGCAACGCCGTCGCTATCTGCTGACGGGTGGCACTGCGTCGACGGCGCTGCCGGACTATGGCAGCGAAGCG
CCGCTGCCGAAATACGAAGATGTCGACGGTGGCGCTGCCGCGACGCCCGCATCGCCGGCTGCGGCGTCGGCGCCCGAAGC
GGCGTCGGGTGCGACGTCGAGCGGTGCAACGGGTGGCGCGGCGGGTGCTGCGCAACCGGCATCGGACACGAATGCACCGC
CGACCGTTCCGGGCGGCCAGATCGTGCCCCCTGCGCACTTCGGCTACCCGTCGCTGAAACTGCGTTAA

Upstream 100 bases:

>100_bases
TCCGGTGCACCCGCTGCGGCGCCCGAGCCTGCAGCACCGGCTCCCGCAGCGCCTGCTGCGGCCGTTTCGGGTGCATCCGG
GCAATAAGGAGAGAACAACA

Downstream 100 bases:

>100_bases
CGGCATGTCAAATGCGGGAAATGTATCTGTGTGCCGTAACACTCGGATACGAATCTCGCAGTTTCGGCGTGGATTGCGTC
TGAACTCGCGTGATATTGTC

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MMTTRLRVAGIALAVATFAGCATVQNPTKEDPIEGFNRTIFTFNDTVDKYALKPVAKGYVFVTPQPVRDSVTNFFSNIGD
VYIAANNLLQLKIADGVSDIMRIVINTIFGVGGLFDVATIAKLPKHDNDLGLTLGHYGVPPGPYLVLPLFGPSTVRDGVG
MVGNYFINPLTYVQPDSLSWALYGLNVVNTRANLLSASDVLEGAALDKYSFVRNAYLQRRRYLLTGGTASTALPDYGSEA
PLPKYEDVDGGAAATPASPAAASAPEAASGATSSGATGGAAGAAQPASDTNAPPTVPGGQIVPPAHFGYPSLKLR

Sequences:

>Translated_315_residues
MMTTRLRVAGIALAVATFAGCATVQNPTKEDPIEGFNRTIFTFNDTVDKYALKPVAKGYVFVTPQPVRDSVTNFFSNIGD
VYIAANNLLQLKIADGVSDIMRIVINTIFGVGGLFDVATIAKLPKHDNDLGLTLGHYGVPPGPYLVLPLFGPSTVRDGVG
MVGNYFINPLTYVQPDSLSWALYGLNVVNTRANLLSASDVLEGAALDKYSFVRNAYLQRRRYLLTGGTASTALPDYGSEA
PLPKYEDVDGGAAATPASPAAASAPEAASGATSSGATGGAAGAAQPASDTNAPPTVPGGQIVPPAHFGYPSLKLR
>Mature_315_residues
MMTTRLRVAGIALAVATFAGCATVQNPTKEDPIEGFNRTIFTFNDTVDKYALKPVAKGYVFVTPQPVRDSVTNFFSNIGD
VYIAANNLLQLKIADGVSDIMRIVINTIFGVGGLFDVATIAKLPKHDNDLGLTLGHYGVPPGPYLVLPLFGPSTVRDGVG
MVGNYFINPLTYVQPDSLSWALYGLNVVNTRANLLSASDVLEGAALDKYSFVRNAYLQRRRYLLTGGTASTALPDYGSEA
PLPKYEDVDGGAAATPASPAAASAPEAASGATSSGATGGAAGAAQPASDTNAPPTVPGGQIVPPAHFGYPSLKLR

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=231, Percent_Identity=37.6623376623377, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 32855; Mature: 32855

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTRLRVAGIALAVATFAGCATVQNPTKEDPIEGFNRTIFTFNDTVDKYALKPVAKGYV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEE
FVTPQPVRDSVTNFFSNIGDVYIAANNLLQLKIADGVSDIMRIVINTIFGVGGLFDVATI
EECCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHH
AKLPKHDNDLGLTLGHYGVPPGPYLVLPLFGPSTVRDGVGMVGNYFINPLTYVQPDSLSW
HHCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCEE
ALYGLNVVNTRANLLSASDVLEGAALDKYSFVRNAYLQRRRYLLTGGTASTALPDYGSEA
EEEECEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCC
PLPKYEDVDGGAAATPASPAAASAPEAASGATSSGATGGAAGAAQPASDTNAPPTVPGGQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IVPPAHFGYPSLKLR
CCCCCCCCCCCCEEC
>Mature Secondary Structure
MMTTRLRVAGIALAVATFAGCATVQNPTKEDPIEGFNRTIFTFNDTVDKYALKPVAKGYV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEE
FVTPQPVRDSVTNFFSNIGDVYIAANNLLQLKIADGVSDIMRIVINTIFGVGGLFDVATI
EECCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHH
AKLPKHDNDLGLTLGHYGVPPGPYLVLPLFGPSTVRDGVGMVGNYFINPLTYVQPDSLSW
HHCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCEE
ALYGLNVVNTRANLLSASDVLEGAALDKYSFVRNAYLQRRRYLLTGGTASTALPDYGSEA
EEEECEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCC
PLPKYEDVDGGAAATPASPAAASAPEAASGATSSGATGGAAGAAQPASDTNAPPTVPGGQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IVPPAHFGYPSLKLR
CCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]