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The map label for this gene is cspA [H]

Identifier: 186477475

GI number: 186477475

Start: 3082948

End: 3083151

Strand: Direct

Name: cspA [H]

Synonym: Bphy_2727

Alternate gene names: 186477475

Gene position: 3082948-3083151 (Clockwise)

Preceding gene: 186477473

Following gene: 186477477

Centisome position: 88.61

GC content: 55.88

Gene sequence:

>204_bases
ATGGAAACCGGTACCGTCAAGTGGTTCAATGACGCAAAGGGCTTTGGCTTCATCACCCCGGACGCTGGCGGCGAAGATCT
GTTCGCGCATTTCTCGGAAATCCGCGTCGAAGGCTTCAAGACGCTGCAGGAAAACCAAAAGGTTACCTACGACGTCAAGA
CGGGCCCGAAGGGCAAGCAAGCCGCTAACATCAAGCCGGCCTAA

Upstream 100 bases:

>100_bases
TACTCCGGTTGGTCCCATTACCCTTCCTTGATTTTCATGCACCCTCGGGCTACGGCCTTTCTGGCTTCGGCCTTGTTCAC
CATTTTTAGGAAAAAGTAAT

Downstream 100 bases:

>100_bases
GCTTCGCTTTCTTCCGGAACGCAAAAACCCCGCCTCGGCGGGGTTTTTTGTTTTTCGGCCTCAATCGCTTTGTCGGCGTG
AACGCTGAATTCTCTATTGG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
METGTVKWFNDAKGFGFITPDAGGEDLFAHFSEIRVEGFKTLQENQKVTYDVKTGPKGKQAANIKPA

Sequences:

>Translated_67_residues
METGTVKWFNDAKGFGFITPDAGGEDLFAHFSEIRVEGFKTLQENQKVTYDVKTGPKGKQAANIKPA
>Mature_67_residues
METGTVKWFNDAKGFGFITPDAGGEDLFAHFSEIRVEGFKTLQENQKVTYDVKTGPKGKQAANIKPA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=60.6060606060606, Blast_Score=93, Evalue=3e-21,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=57.3770491803279, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=56.4516129032258, Blast_Score=79, Evalue=4e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=56.4516129032258, Blast_Score=79, Evalue=7e-17,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=56.4516129032258, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=54.8387096774194, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=55.7377049180328, Blast_Score=75, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983225, Length=56, Percent_Identity=44.6428571428571, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71983217, Length=56, Percent_Identity=44.6428571428571, Blast_Score=62, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=40.9836065573771, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7363; Mature: 7363

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGTVKWFNDAKGFGFITPDAGGEDLFAHFSEIRVEGFKTLQENQKVTYDVKTGPKGKQ
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
AANIKPA
CCCCCCC
>Mature Secondary Structure
METGTVKWFNDAKGFGFITPDAGGEDLFAHFSEIRVEGFKTLQENQKVTYDVKTGPKGKQ
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
AANIKPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA