Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 186477419

GI number: 186477419

Start: 3012766

End: 3013707

Strand: Reverse

Name: ddl

Synonym: Bphy_2671

Alternate gene names: 186477419

Gene position: 3013707-3012766 (Counterclockwise)

Preceding gene: 186477420

Following gene: 186477418

Centisome position: 86.62

GC content: 64.01

Gene sequence:

>942_bases
ATGAGCGGTATCGATCCTAAATCTTTCGGCAAGGTTGCCGTGCTGCTCGGCGGTGCGTCCGCCGAGCGCGAAGTGTCGCT
GAACTCCGGCCGTCTCGTGCTGCAAGGCCTGCGCGATGCAGGCGTCGACGCGTATGCGTTCGATCCCGCCGAGCGTCCGC
TCGCGGCATTGAAGGATGAAGGCTTCGTGCGCGCGTTCAACGCGCTGCACGGCGGCTATGGCGAGAACGGCCAGATTCAG
GGCGCGCTCGATTTCTACGGTATCCGTTATACGGGCAGCGGCGTCCTCGGCTCGGCGCTCGGTCTCGACAAGTTCCGCAC
CAAGCTCGTGTGGCAGCAGCTCGGCATTCCGACGCCGCCGTTCGAAGCCGTGCTGCGCGGCGACGACTACGCCGCGCGCG
CGCAGGGCATCGTCGCGAAGCTCGGCTTGCCGCTCTTCGTGAAGCCGGCCAGCGAAGGGTCGAGCGTTGCCGTCATCAAG
GTGAAGACGGCCGACGCGCTCGTGCCCGCGCTCGAAGAAGCGGTGAAGTTCGACAAGATCGTCGTAGTGGAAAAGAGCAT
CGAAGGCGGCGGCGAGTACACGGCGTGCATCGCGGGCGATCTCGATCTGCCCATCATCCGCATCGTGCCCGCCGGCGAGT
TCTACGACTATCACGCGAAGTACATCGCGAACGACACGCAGTACATGATTCCGTGCGGCATCGCGGCTGAAGAAGAAGCG
CGCCTGAAGAAGCTCGCGCGCCAGGCGTTCGACGTGCTCGGCTGCACCGACTGGGGTCGTGCCGACTTCATGCTGGACGG
AGAAGGCAACGCATATTTTCTCGAAGTGAACACGGCGCCCGGCATGACGGATCATTCGCTGCCGCCGAAGGCAGCGCGTG
CTGTCGGCATCAGCTACCAGGAACTGGTGGTGAAGGTGCTCGCGCTGACGCTGAAGGATTAA

Upstream 100 bases:

>100_bases
CGCTCGGTGCAATCGTGCGCGACGGCGATGTGGTGATCACGATGGGTGCGGGTTCGATCGGGAGTGTGCCGGGTCGGCTC
GCAGGAAACGAAGGTGTGAA

Downstream 100 bases:

>100_bases
GGAACACGCCTCGACATGTGGAACAACGTTCGCCAGCTCAACCTCGCCGCCAACGCGTTGCACGCGTTGTTGCTGCTCGT
GCTGCTGGCGGCGGGCGGCT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSGIDPKSFGKVAVLLGGASAEREVSLNSGRLVLQGLRDAGVDAYAFDPAERPLAALKDEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYAARAQGIVAKLGLPLFVKPASEGSSVAVIK
VKTADALVPALEEAVKFDKIVVVEKSIEGGGEYTACIAGDLDLPIIRIVPAGEFYDYHAKYIANDTQYMIPCGIAAEEEA
RLKKLARQAFDVLGCTDWGRADFMLDGEGNAYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVKVLALTLKD

Sequences:

>Translated_313_residues
MSGIDPKSFGKVAVLLGGASAEREVSLNSGRLVLQGLRDAGVDAYAFDPAERPLAALKDEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYAARAQGIVAKLGLPLFVKPASEGSSVAVIK
VKTADALVPALEEAVKFDKIVVVEKSIEGGGEYTACIAGDLDLPIIRIVPAGEFYDYHAKYIANDTQYMIPCGIAAEEEA
RLKKLARQAFDVLGCTDWGRADFMLDGEGNAYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVKVLALTLKD
>Mature_312_residues
SGIDPKSFGKVAVLLGGASAEREVSLNSGRLVLQGLRDAGVDAYAFDPAERPLAALKDEGFVRAFNALHGGYGENGQIQG
ALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYAARAQGIVAKLGLPLFVKPASEGSSVAVIKV
KTADALVPALEEAVKFDKIVVVEKSIEGGGEYTACIAGDLDLPIIRIVPAGEFYDYHAKYIANDTQYMIPCGIAAEEEAR
LKKLARQAFDVLGCTDWGRADFMLDGEGNAYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVKVLALTLKD

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=50.9933774834437, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=32.7586206896552, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BURP8 (B2JHF8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858889.1
- ProteinModelPortal:   B2JHF8
- SMR:   B2JHF8
- GeneID:   6244154
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2671
- HOGENOM:   HBG427092
- OMA:   NLSNALE
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33342; Mature: 33211

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIDPKSFGKVAVLLGGASAEREVSLNSGRLVLQGLRDAGVDAYAFDPAERPLAALKDE
CCCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEHHHCCCCEEEECCCCCCHHHHCCC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHCCCCCC
FEAVLRGDDYAARAQGIVAKLGLPLFVKPASEGSSVAVIKVKTADALVPALEEAVKFDKI
HHHHCCCCCHHHHHHCHHHHCCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHCEE
VVVEKSIEGGGEYTACIAGDLDLPIIRIVPAGEFYDYHAKYIANDTQYMIPCGIAAEEEA
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHCCHHEEECCCCEEEECCCCCCHHH
RLKKLARQAFDVLGCTDWGRADFMLDGEGNAYFLEVNTAPGMTDHSLPPKAARAVGISYQ
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLALTLKD
HHHHHHHHHHCCC
>Mature Secondary Structure 
SGIDPKSFGKVAVLLGGASAEREVSLNSGRLVLQGLRDAGVDAYAFDPAERPLAALKDE
CCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEHHHCCCCEEEECCCCCCHHHHCCC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHCCCCCC
FEAVLRGDDYAARAQGIVAKLGLPLFVKPASEGSSVAVIKVKTADALVPALEEAVKFDKI
HHHHCCCCCHHHHHHCHHHHCCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHCEE
VVVEKSIEGGGEYTACIAGDLDLPIIRIVPAGEFYDYHAKYIANDTQYMIPCGIAAEEEA
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHCCHHEEECCCCEEEECCCCCCHHH
RLKKLARQAFDVLGCTDWGRADFMLDGEGNAYFLEVNTAPGMTDHSLPPKAARAVGISYQ
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLALTLKD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA