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The map label for this gene is gspO [H]

Identifier: 186477405

GI number: 186477405

Start: 2998222

End: 2999151

Strand: Direct

Name: gspO [H]

Synonym: Bphy_2657

Alternate gene names: 186477405

Gene position: 2998222-2999151 (Clockwise)

Preceding gene: 186477404

Following gene: 186477406

Centisome position: 86.18

GC content: 63.76

Gene sequence:

>930_bases
ATGCCGATCCCGCTCACGTCCGTACCCGAACCCTCCCTTAGCGGCCCGCTCGCCCGTTTCGCGGACAACCTCAGCGCGGC
CTTCGACATGTTGCCGGCCGCTGCGCAGATCGCGTTCGTGATCGTCTTCGGCCTCGTGATTGGCAGCTTTCTGAACGTCG
TCGTGCATCGGCTGCCGATCATGCTCGAACGCGCATGGCGCTCGGAAGTCAACGAAGCAACGGGCCACGCATTCGACGAA
GACGGCCTGCCCGAACGCTACAACCTGTGGTTGCCGCGCAGCGCATGTCCTCATTGTGCTCATGTGCTGCGCACGTGGGA
GAACATTCCCGTGTTCAGCTATCTGCTGTTGCGCGGTCGCTGCTCGCAATGCAAGGGGCGCGTGAGCTTTCGGTATCCGC
TGCTCGAACTGTCGAGCGCGGCGCTGGCGCTGGGTGCGCTCGTCGCGTTCGGTGCAACGGGCTCGGCGCTCGCTGCGTTC
GGCCTGTGTGCGACGCTGCTGGCGATGAGCGCGATCGATATCGACACGCACCTGTTGCCCGATTCGATGACCTTGCCGTT
GCTGTGGGCCGGCCTCATCGTCAATTTCAATGCGGTGTTCGCGACCCTGCACGATGCCGTAGTCGGCGCGATCGCCGGTT
ACCTCGCATTGTGGTGCGTGCATTGGCTGTTCAAGATCGTGCGCGGTGTCGAAGGGATGGGATACGGCGATTTCAAGCTG
CTCTCCGCACTGGGTGCGTGGCTTGGCTGGACTGCGCTGCCGCAGATCATTCTGATCGCGGCGGTGACAGGTGCCGTCGT
GGGCCTGTTTGCAACGTGGATCGGCCGCATGCGGTTCGAAGAGCCGCTGCCATTCGGTCCGTTTCTCGCGGCGGGCGGCG
CGGTGACGCTCTTTGTCGGCACGCCGCTTTACATGGCTTTGGGAGGCTGA

Upstream 100 bases:

>100_bases
GGCGTGCTCGTCGGCGGTCTTGTCGTCGCGATGTATCTTCCCATTATCCAACTCGGCAACGTGGTGTAGTAGCATCGCAG
CCGAATCCCGACAATCGATC

Downstream 100 bases:

>100_bases
CGCATGTTTGCTGTGGGATTGACGGGCGGCATCGGCAGCGGCAAATCTACGGTAGGCGATCTGTTCGCGAAACGTGGCGT
GACACTCGTCGATACCGACG

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPIPLTSVPEPSLSGPLARFADNLSAAFDMLPAAAQIAFVIVFGLVIGSFLNVVVHRLPIMLERAWRSEVNEATGHAFDE
DGLPERYNLWLPRSACPHCAHVLRTWENIPVFSYLLLRGRCSQCKGRVSFRYPLLELSSAALALGALVAFGATGSALAAF
GLCATLLAMSAIDIDTHLLPDSMTLPLLWAGLIVNFNAVFATLHDAVVGAIAGYLALWCVHWLFKIVRGVEGMGYGDFKL
LSALGAWLGWTALPQIILIAAVTGAVVGLFATWIGRMRFEEPLPFGPFLAAGGAVTLFVGTPLYMALGG

Sequences:

>Translated_309_residues
MPIPLTSVPEPSLSGPLARFADNLSAAFDMLPAAAQIAFVIVFGLVIGSFLNVVVHRLPIMLERAWRSEVNEATGHAFDE
DGLPERYNLWLPRSACPHCAHVLRTWENIPVFSYLLLRGRCSQCKGRVSFRYPLLELSSAALALGALVAFGATGSALAAF
GLCATLLAMSAIDIDTHLLPDSMTLPLLWAGLIVNFNAVFATLHDAVVGAIAGYLALWCVHWLFKIVRGVEGMGYGDFKL
LSALGAWLGWTALPQIILIAAVTGAVVGLFATWIGRMRFEEPLPFGPFLAAGGAVTLFVGTPLYMALGG
>Mature_308_residues
PIPLTSVPEPSLSGPLARFADNLSAAFDMLPAAAQIAFVIVFGLVIGSFLNVVVHRLPIMLERAWRSEVNEATGHAFDED
GLPERYNLWLPRSACPHCAHVLRTWENIPVFSYLLLRGRCSQCKGRVSFRYPLLELSSAALALGALVAFGATGSALAAFG
LCATLLAMSAIDIDTHLLPDSMTLPLLWAGLIVNFNAVFATLHDAVVGAIAGYLALWCVHWLFKIVRGVEGMGYGDFKLL
SALGAWLGWTALPQIILIAAVTGAVVGLFATWIGRMRFEEPLPFGPFLAAGGAVTLFVGTPLYMALGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=273, Percent_Identity=40.2930402930403, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=50, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33080; Mature: 32949

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIPLTSVPEPSLSGPLARFADNLSAAFDMLPAAAQIAFVIVFGLVIGSFLNVVVHRLPI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLERAWRSEVNEATGHAFDEDGLPERYNLWLPRSACPHCAHVLRTWENIPVFSYLLLRGR
HHHHHHHHHHHHHHCCCCCCCCCCCHHCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
CSQCKGRVSFRYPLLELSSAALALGALVAFGATGSALAAFGLCATLLAMSAIDIDTHLLP
HHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
DSMTLPLLWAGLIVNFNAVFATLHDAVVGAIAGYLALWCVHWLFKIVRGVEGMGYGDFKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LSALGAWLGWTALPQIILIAAVTGAVVGLFATWIGRMRFEEPLPFGPFLAAGGAVTLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEEC
TPLYMALGG
CCHHHHCCC
>Mature Secondary Structure 
PIPLTSVPEPSLSGPLARFADNLSAAFDMLPAAAQIAFVIVFGLVIGSFLNVVVHRLPI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLERAWRSEVNEATGHAFDEDGLPERYNLWLPRSACPHCAHVLRTWENIPVFSYLLLRGR
HHHHHHHHHHHHHHCCCCCCCCCCCHHCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
CSQCKGRVSFRYPLLELSSAALALGALVAFGATGSALAAFGLCATLLAMSAIDIDTHLLP
HHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
DSMTLPLLWAGLIVNFNAVFATLHDAVVGAIAGYLALWCVHWLFKIVRGVEGMGYGDFKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LSALGAWLGWTALPQIILIAAVTGAVVGLFATWIGRMRFEEPLPFGPFLAAGGAVTLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEEC
TPLYMALGG
CCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]