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The map label for this gene is gdhA [H]

Identifier: 186477322

GI number: 186477322

Start: 2893935

End: 2895227

Strand: Reverse

Name: gdhA [H]

Synonym: Bphy_2574

Alternate gene names: 186477322

Gene position: 2895227-2893935 (Counterclockwise)

Preceding gene: 186477323

Following gene: 186477321

Centisome position: 83.22

GC content: 63.19

Gene sequence:

>1293_bases
ATGTCTACCGCGGCAAATGTGCAGTCCATCCCGTCCTACCTTCACCAGGATGATCTCGGCCCCTGGGGCAACTATCTCCG
TCAAGTCGATCGCGTTGCGCCGTACCTCGGCTCGCTGTCGCGCTGGCTCGAAACGCTGAAGCGCCCGAAGCGCATTCTGG
TCGTCGACGTGCCCATCGAGCTCGACAACGGCACGGTTGCGCATTTCGAAGGCTATCGCGTGCAGCACAATGTGTCGCGC
GGTCCGGGCAAGGGTGGCGTGCGTTATCACCAGGACGTGACGCTGTCTGAAGTGATGGCGCTGTCCGCGTGGATGTCGGT
GAAGAACGCGGCCGTCAACGTGCCGTACGGCGGTGCGAAGGGCGGCATCCGCGTCGATCCGCGCACGCTGTCGCGTGGCG
AACTCGAGCGTGTCACACGCCGCTACACGAGCGAAATCGGCATCATCATCGGACCGAATACCGACATCCCCGCGCCGGAC
GTGAACACGAACGAGCAGATCATGGCGTGGATGATGGATACGTACTCGATGAACCAGGGCCAAACAGCAACGGGTGTCGT
GACGGGCAAGCCGATCACGCTCGGCGGTTCGCTCGGCCGTCGTGAAGCAACGGGCCGCGGCGTGTTCGTCGTCGGCTGCG
AAGCGGCGCGCCGCATCGGCATGGACATCGAAGGCGCGCGCATCGCCGTGCAGGGCTTCGGCAACGTCGGCGGCATCGCG
GCGCGTCTTTATCAGGAAGCGGGCGCGAAGGTCGTTGCCGTGCAGGATCACACGGGCACGCTGTATAAGGAATCGGGCAT
CGACGCAGTCGCACTGCTTGAGCACGTCGCGAGGCACGGCGGTGTCGGCGGCTACGCTGAAGCCGACACGATCGCGAACG
AAGACTTCTGGGCGATCGAATCGGACATTCTGATTCCCGCCGCGCTCGAAAACCAGATCACCGAGAAGAACGCAGGCAAG
ATTCGTACGAAGATCATCGTCGAAGGCGCGAACGGCCCGACCACGACCGCCGCAGACGACATTCTGCACGACAAGGGGAT
TCTCGTGATCCCCGACGTGGTCGCGAATGCGGGTGGCGTGACGGTTTCGTACTTCGAGTGGGTGCAGGACTTCTCGAGCT
TCTTCTGGACGGAAGACGAGATCAACGAGCGCCTCGAGCGCGTTATGCGCGAAGCCTTCGCTGCCGTGTGGCAGGTCGCG
AGCGAGCAGAAGGTGTCGGTGCGCACCGCGGCGTTCATCGTTGCTTGCAAGCGCATCCTTCAAGCGCGTGAAATGCGCGG
CCTGTATCCCTGA

Upstream 100 bases:

>100_bases
GGTTATGCAAGAAAAACATAACCGGATGACCAAACGGCATTGGACCCATCCAGCGCTTTTTTTCGACAATGGCGTCACCC
GATAAAACGCTGGAGCGTTC

Downstream 100 bases:

>100_bases
TACATGGACGCGCCCGCGTATATCCGATACAGGGCGAAACTGGCGCCCGTCGCGGACGCGGCGCCAGGCAACGGCGTTTT
TCCAGCGCGATTTCACGAAG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSTAANVQSIPSYLHQDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQHNVSR
GPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPD
VNTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAARRIGMDIEGARIAVQGFGNVGGIA
ARLYQEAGAKVVAVQDHTGTLYKESGIDAVALLEHVARHGGVGGYAEADTIANEDFWAIESDILIPAALENQITEKNAGK
IRTKIIVEGANGPTTTAADDILHDKGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINERLERVMREAFAAVWQVA
SEQKVSVRTAAFIVACKRILQAREMRGLYP

Sequences:

>Translated_430_residues
MSTAANVQSIPSYLHQDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQHNVSR
GPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPD
VNTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAARRIGMDIEGARIAVQGFGNVGGIA
ARLYQEAGAKVVAVQDHTGTLYKESGIDAVALLEHVARHGGVGGYAEADTIANEDFWAIESDILIPAALENQITEKNAGK
IRTKIIVEGANGPTTTAADDILHDKGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINERLERVMREAFAAVWQVA
SEQKVSVRTAAFIVACKRILQAREMRGLYP
>Mature_429_residues
STAANVQSIPSYLHQDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQHNVSRG
PGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPDV
NTNEQIMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAARRIGMDIEGARIAVQGFGNVGGIAA
RLYQEAGAKVVAVQDHTGTLYKESGIDAVALLEHVARHGGVGGYAEADTIANEDFWAIESDILIPAALENQITEKNAGKI
RTKIIVEGANGPTTTAADDILHDKGILVIPDVVANAGGVTVSYFEWVQDFSSFFWTEDEINERLERVMREAFAAVWQVAS
EQKVSVRTAAFIVACKRILQAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=43.731778425656, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=43.4402332361516, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=34.7222222222222, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=392, Percent_Identity=38.7755102040816, Blast_Score=258, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=30.050505050505, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=380, Percent_Identity=30, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45553475, Length=361, Percent_Identity=44.0443213296399, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45549226, Length=334, Percent_Identity=45.8083832335329, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24649283, Length=350, Percent_Identity=44.2857142857143, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24649460, Length=281, Percent_Identity=46.6192170818505, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46723; Mature: 46591

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAANVQSIPSYLHQDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIE
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
LDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAK
ECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCC
GGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQIMAWMMDTYSMNQG
CCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAARRIGMDIEGARIAVQGFGNVGGIA
CCEEEEEECCCEEECCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCEEEEEECCCCHHHH
ARLYQEAGAKVVAVQDHTGTLYKESGIDAVALLEHVARHGGVGGYAEADTIANEDFWAIE
HHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
SDILIPAALENQITEKNAGKIRTKIIVEGANGPTTTAADDILHDKGILVIPDVVANAGGV
CCCEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCEEECHHHCCCCCC
TVSYFEWVQDFSSFFWTEDEINERLERVMREAFAAVWQVASEQKVSVRTAAFIVACKRIL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QAREMRGLYP
HHHHHCCCCC
>Mature Secondary Structure 
STAANVQSIPSYLHQDDLGPWGNYLRQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
LDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAK
ECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECCCCCCC
GGIRVDPRTLSRGELERVTRRYTSEIGIIIGPNTDIPAPDVNTNEQIMAWMMDTYSMNQG
CCEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAARRIGMDIEGARIAVQGFGNVGGIA
CCEEEEEECCCEEECCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCEEEEEECCCCHHHH
ARLYQEAGAKVVAVQDHTGTLYKESGIDAVALLEHVARHGGVGGYAEADTIANEDFWAIE
HHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
SDILIPAALENQITEKNAGKIRTKIIVEGANGPTTTAADDILHDKGILVIPDVVANAGGV
CCCEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCEEECHHHCCCCCC
TVSYFEWVQDFSSFFWTEDEINERLERVMREAFAAVWQVASEQKVSVRTAAFIVACKRIL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QAREMRGLYP
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]