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The map label for this gene is queA

Identifier: 186477272

GI number: 186477272

Start: 2844794

End: 2845855

Strand: Direct

Name: queA

Synonym: Bphy_2524

Alternate gene names: 186477272

Gene position: 2844794-2845855 (Clockwise)

Preceding gene: 186477266

Following gene: 186477273

Centisome position: 81.77

GC content: 65.54

Gene sequence:

>1062_bases
ATGTTCACGCTTTCCGATTTCGATTTCGACCTGCCGCCCGAGCTGATCGCGCAAACCGCCCTGCCCGAGCGCAGCGCGAG
CCGTCTGCTCGAAGTGAACGGGCAAACCACGCCCGCGTCGTTCGCCGACCGCCGCTTCGCCGAACTGCCCGAGTGCATCC
AGCCCGGCGACCTGCTCGTCTTCAACGATACCAAAGTGCTGAAGGCCCGTTTCCTCGGCCAGAAGGCCAGCGGCGGCAAG
ATCGAGGTGCTGGTCGAGCGGCTGACTGGCGAACGCACGGCGCTCGCGCAGATTCGCGCGAGCAAGAGTCCGGGGCCGGG
CACTGTGCTGCGTCTCGCCGATGCCTTCGATGTCACTGTCGGCGAGCGCGTCGAGCCGTTCTACACGTTGCACTTCCCCG
ACGACTGCCTGACGCTGATCGAGCAGTTCGGCCGTCTGCCGCTGCCGCCGTATATCGAGCACGACCCCGACGCATTCGAC
GAAACGCGCTATCAGACGGTGTACGCGCAAAATCCGGGTGCCGTCGCCGCACCGACGGCCGGCCTGCATTTCGACGATTC
GATCTTCGCGCGGCTGGATGCAAAAGGCGTCGAACGTGCGACGCTCACGCTGCATGTAGGCGCGGGCACGTTCCAGCCGG
TCCGCGTCGAGAACATCGCCGAACACAAGATGCATAGCGAGTGGTATCAGCTGCCGCAATCGCTCGTCGACAGGATCGCC
GCGACCAGGGCGCGTGGCAATCGCGTGATCGCGGTCGGCACGACGTCGATGCGCGCGCTGGAAGCCGCCGCGCGCGACGC
CGACGCTGCAGGCAAGCCGCTCGCCGCCACCAGCGCCGAGACGGACATTTTCATCACGCCGGGCTACAAGTTCCGCGTGG
TCGACCGGCTCGTCACGAATTTCCACCTGCCGAAGTCGACGCTGCTGATGCTGGTGTCGGCGTTCGCGGGCATAGAAACG
ATCCGCGCGGCGTATCGTCACGCGATCGACGGGCGTTACCGCTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGCAG
CGAAGACGCACTGAACGCATAA

Upstream 100 bases:

>100_bases
GCTGTCGTAGGCGCTGATGCTTCAGCCGTCATTTCGGCTTCGCCCTCGCCGGGCGGCGCTCCCGCTTCGCACTGTTCCAA
CCCGCATCAAGTCAGTCCGC

Downstream 100 bases:

>100_bases
TCCTTCACTCGCTGCCGGAAACGCTCCCGCAGCATTCTTCATCATGCGCCGGACTGTTTTCCGGTCTGCACACGGAGTCA
ACCCATGACCGACGGTCATC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MFTLSDFDFDLPPELIAQTALPERSASRLLEVNGQTTPASFADRRFAELPECIQPGDLLVFNDTKVLKARFLGQKASGGK
IEVLVERLTGERTALAQIRASKSPGPGTVLRLADAFDVTVGERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDAFD
ETRYQTVYAQNPGAVAAPTAGLHFDDSIFARLDAKGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYQLPQSLVDRIA
ATRARGNRVIAVGTTSMRALEAAARDADAAGKPLAATSAETDIFITPGYKFRVVDRLVTNFHLPKSTLLMLVSAFAGIET
IRAAYRHAIDGRYRFFSYGDAMLLTRSEDALNA

Sequences:

>Translated_353_residues
MFTLSDFDFDLPPELIAQTALPERSASRLLEVNGQTTPASFADRRFAELPECIQPGDLLVFNDTKVLKARFLGQKASGGK
IEVLVERLTGERTALAQIRASKSPGPGTVLRLADAFDVTVGERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDAFD
ETRYQTVYAQNPGAVAAPTAGLHFDDSIFARLDAKGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYQLPQSLVDRIA
ATRARGNRVIAVGTTSMRALEAAARDADAAGKPLAATSAETDIFITPGYKFRVVDRLVTNFHLPKSTLLMLVSAFAGIET
IRAAYRHAIDGRYRFFSYGDAMLLTRSEDALNA
>Mature_353_residues
MFTLSDFDFDLPPELIAQTALPERSASRLLEVNGQTTPASFADRRFAELPECIQPGDLLVFNDTKVLKARFLGQKASGGK
IEVLVERLTGERTALAQIRASKSPGPGTVLRLADAFDVTVGERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDAFD
ETRYQTVYAQNPGAVAAPTAGLHFDDSIFARLDAKGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYQLPQSLVDRIA
ATRARGNRVIAVGTTSMRALEAAARDADAAGKPLAATSAETDIFITPGYKFRVVDRLVTNFHLPKSTLLMLVSAFAGIET
IRAAYRHAIDGRYRFFSYGDAMLLTRSEDALNA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=353, Percent_Identity=52.4079320113314, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BURP8 (B2JGG8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858742.1
- GeneID:   6244007
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2524
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38812; Mature: 38812

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLSDFDFDLPPELIAQTALPERSASRLLEVNGQTTPASFADRRFAELPECIQPGDLLV
CCCCCCCCCCCCHHHHHHHCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
FNDTKVLKARFLGQKASGGKIEVLVERLTGERTALAQIRASKSPGPGTVLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECHHCCCC
GERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDAFDETRYQTVYAQNPGAVAAPTA
CCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCC
GLHFDDSIFARLDAKGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYQLPQSLVDRIA
CCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATRARGNRVIAVGTTSMRALEAAARDADAAGKPLAATSAETDIFITPGYKFRVVDRLVTN
HHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGIETIRAAYRHAIDGRYRFFSYGDAMLLTRSEDALNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHCCC
>Mature Secondary Structure
MFTLSDFDFDLPPELIAQTALPERSASRLLEVNGQTTPASFADRRFAELPECIQPGDLLV
CCCCCCCCCCCCHHHHHHHCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
FNDTKVLKARFLGQKASGGKIEVLVERLTGERTALAQIRASKSPGPGTVLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECHHCCCC
GERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDAFDETRYQTVYAQNPGAVAAPTA
CCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCC
GLHFDDSIFARLDAKGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYQLPQSLVDRIA
CCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATRARGNRVIAVGTTSMRALEAAARDADAAGKPLAATSAETDIFITPGYKFRVVDRLVTN
HHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGIETIRAAYRHAIDGRYRFFSYGDAMLLTRSEDALNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA