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The map label for this gene is glcF [H]

Identifier: 186477264

GI number: 186477264

Start: 2834511

End: 2835737

Strand: Direct

Name: glcF [H]

Synonym: Bphy_2516

Alternate gene names: 186477264

Gene position: 2834511-2835737 (Clockwise)

Preceding gene: 186477263

Following gene: 186477265

Centisome position: 81.47

GC content: 63.24

Gene sequence:

>1227_bases
ATGCAAACCAATCTCGCCGACTTCATTCGCAATACACCGGACGGTGACGAAGCCGACGCCATCCTGCGCAAATGCGTGCA
TTGCGGCTTCTGCACAGCGACCTGCCCGACCTATCAGCTGCTGGGCGACGAACTCGACGGTCCGCGCGGCCGAATCTATC
TGATCAAGCAGATGGTGGAAGGCGCGCCCGTCACGCGCAGCACGCAGTTGCACCTGGATCGTTGCCTGACTTGCCGCAAT
TGCGAATCCACCTGCCCTTCCGGCGTCCAGTACGGCAAGCTCGTCGAGATAGGCCGCAAGCTCACTGAAGAGAAAGTGCC
GCGCCCGTTTGGCCAGCGCCTGATGCGCCGCTTGCTCGCGAGCTTCGTGCCGAACAGCACGCTGTTCACCCCCGCGATGC
GGCTCGGTCAGCACTTTCGCCCGCTGCTGCCGAAGAAGCTGCGGGACAAGGTGCCGCAACGTCAGCGCCAGCTCGAATGG
CCGACGGCCACGCATCCGCGCAAGATGCTGATGCTCGCAGGGTGTGTGCAGCCTTCGATGATGCCGAACGTCAACATTGC
GACGGCGCGCGTGCTGGACGCGCTCGGCGTCCAGACGGTGATCGCACCGGAAGCGGGTTGTTGCGGCGCGATCCGCCTGC
ACCTCGGCTACAACGACGAGGCGCTCGACGACGTGCGCCAGAACATCGACGCATGGTGGCCGCATGTCGAGCAAGGTGCG
GAAGCGATCGTGATGAATGCGTCTGGTTGCGGCGCGACTGTGAAGGAGTATGCCCACCTGATGCGCAACGACCCGGCCTA
TGCGGAGAAGGCGAAACGCATTGTCGAGCTGACGCGCGACCTCAGCGAGATTCTGCCGGACTTCGAAGAAGCGCTGACGC
CGCTCACGCGGCGCCGGGCGATTCATACGGTTGCGTATCACCCGCCCTGCACGCTTCAGCATGGCCAGCAGATACGCGGC
AAGGTCGAGCATCTTCTGACCGCGCTGGGCATCGAAGTCAGGCTCCCCGCCGACAGCCATCTGTGCTGTGGCTCCGCAGG
CACCTATTCCCTCACGCAGCCGGCGCTTTCGTACGCATTGCGCAACCAGAAGCTGGAAAAGCTGAAAGCACAGGAGCCGC
AGATCGTCGTGTCCGCGAATGTGGGTTGTATCGCGCATCTGCAAAGCGGCACCTCCATGCCCGTCGTGCATTGGGTCGAA
CTTGTCGAGCACATGCTCGCGTCCTGA

Upstream 100 bases:

>100_bases
CCTGCCCCGCTGATGAAAATCCATCGCGGTCTGAAAGCCACTTTCGACCCGGCCCGCATCTTCAACCGCGGCCGGCTGTA
CCCCGACTTCTGAGCGCGCG

Downstream 100 bases:

>100_bases
CCGCCAGCATCGCTTGCGGCACGTTCGTGCACAACCTGGCCGATCGGCCAGGTCGTGGCGCGCGGGCAGCGTCCGTATAA
TTCGAATCGTCGCCCCTCGC

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQLHLDRCLTCRN
CESTCPSGVQYGKLVEIGRKLTEEKVPRPFGQRLMRRLLASFVPNSTLFTPAMRLGQHFRPLLPKKLRDKVPQRQRQLEW
PTATHPRKMLMLAGCVQPSMMPNVNIATARVLDALGVQTVIAPEAGCCGAIRLHLGYNDEALDDVRQNIDAWWPHVEQGA
EAIVMNASGCGATVKEYAHLMRNDPAYAEKAKRIVELTRDLSEILPDFEEALTPLTRRRAIHTVAYHPPCTLQHGQQIRG
KVEHLLTALGIEVRLPADSHLCCGSAGTYSLTQPALSYALRNQKLEKLKAQEPQIVVSANVGCIAHLQSGTSMPVVHWVE
LVEHMLAS

Sequences:

>Translated_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQLHLDRCLTCRN
CESTCPSGVQYGKLVEIGRKLTEEKVPRPFGQRLMRRLLASFVPNSTLFTPAMRLGQHFRPLLPKKLRDKVPQRQRQLEW
PTATHPRKMLMLAGCVQPSMMPNVNIATARVLDALGVQTVIAPEAGCCGAIRLHLGYNDEALDDVRQNIDAWWPHVEQGA
EAIVMNASGCGATVKEYAHLMRNDPAYAEKAKRIVELTRDLSEILPDFEEALTPLTRRRAIHTVAYHPPCTLQHGQQIRG
KVEHLLTALGIEVRLPADSHLCCGSAGTYSLTQPALSYALRNQKLEKLKAQEPQIVVSANVGCIAHLQSGTSMPVVHWVE
LVEHMLAS
>Mature_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQLHLDRCLTCRN
CESTCPSGVQYGKLVEIGRKLTEEKVPRPFGQRLMRRLLASFVPNSTLFTPAMRLGQHFRPLLPKKLRDKVPQRQRQLEW
PTATHPRKMLMLAGCVQPSMMPNVNIATARVLDALGVQTVIAPEAGCCGAIRLHLGYNDEALDDVRQNIDAWWPHVEQGA
EAIVMNASGCGATVKEYAHLMRNDPAYAEKAKRIVELTRDLSEILPDFEEALTPLTRRRAIHTVAYHPPCTLQHGQQIRG
KVEHLLTALGIEVRLPADSHLCCGSAGTYSLTQPALSYALRNQKLEKLKAQEPQIVVSANVGCIAHLQSGTSMPVVHWVE
LVEHMLAS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=53.3169533169533, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1788576, Length=415, Percent_Identity=23.3734939759036, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=23.4042553191489, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45299; Mature: 45299

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQLHLDRCLTCRNCESTCPSGVQYGKLVEIGRKLTEEKVPRPFGQRLMRRLLA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SFVPNSTLFTPAMRLGQHFRPLLPKKLRDKVPQRQRQLEWPTATHPRKMLMLAGCVQPSM
HHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
MPNVNIATARVLDALGVQTVIAPEAGCCGAIRLHLGYNDEALDDVRQNIDAWWPHVEQGA
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHCCC
EAIVMNASGCGATVKEYAHLMRNDPAYAEKAKRIVELTRDLSEILPDFEEALTPLTRRRA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQIRGKVEHLLTALGIEVRLPADSHLCCGSAGTYSLTQPALSYAL
HHHEEECCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCHHHHHHHHHHH
RNQKLEKLKAQEPQIVVSANVGCIAHLQSGTSMPVVHWVELVEHMLAS
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQLHLDRCLTCRNCESTCPSGVQYGKLVEIGRKLTEEKVPRPFGQRLMRRLLA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SFVPNSTLFTPAMRLGQHFRPLLPKKLRDKVPQRQRQLEWPTATHPRKMLMLAGCVQPSM
HHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
MPNVNIATARVLDALGVQTVIAPEAGCCGAIRLHLGYNDEALDDVRQNIDAWWPHVEQGA
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHCCC
EAIVMNASGCGATVKEYAHLMRNDPAYAEKAKRIVELTRDLSEILPDFEEALTPLTRRRA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQIRGKVEHLLTALGIEVRLPADSHLCCGSAGTYSLTQPALSYAL
HHHEEECCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCHHHHHHHHHHH
RNQKLEKLKAQEPQIVVSANVGCIAHLQSGTSMPVVHWVELVEHMLAS
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]