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The map label for this gene is grpE

Identifier: 186477248

GI number: 186477248

Start: 2811148

End: 2811756

Strand: Reverse

Name: grpE

Synonym: Bphy_2499

Alternate gene names: 186477248

Gene position: 2811756-2811148 (Counterclockwise)

Preceding gene: 186477250

Following gene: 186477247

Centisome position: 80.82

GC content: 66.5

Gene sequence:

>609_bases
ATGGAAAACACGCAAGAGAACCCGACGAGCCAGAACCCTACGCCCGCCGACGAAGCCGCGCGCCAGGCCGCAGAGGCCGC
ATCGGGCGAGCCGCAGGATCAGGCGCGGCAGCCGGCAGCGGCTGCCGGCGAACAGCCGGCGCAGGCTCAACCCGCCGGTG
CCGAAGCCGCGCTGGCCGAGGCGCAGGCGAAGCTGGCCGAACTGCAGGAGAGCTTCCTGCGCGCCAAGGCGGAAACAGAA
AACGTCCGCCGCCGCGGTCAGGAAGACGTGGCGAAGGCACATAAATTCGCGATCGAAAGCTTTGCCGAACATCTGCTGCC
CGTCATGGACAGCCTCGAAGCGGCCGTCGCGCACTCCACCGACGATCTCGCCAAGGTCCGCGAAGGCGTTGAACTCACGT
TGCGCCAGCTGACGGGCGCGCTGGAGAAGGGCAAGGTTGTTGCGCTGAACCCGGTCGGCGAAAAGTTCGATCCGCACCGT
CACCAGGCCATTTCGATGGTTCCGGCCGATCAGGAGCCGAACACGGTCGTCGCTGTGCTGCAAAAGGGCTATGTGATCGC
CGACCGCGTGCTGCGTCCGGCGCTTGTCACGGTCGCAGCGCCGAAGTAA

Upstream 100 bases:

>100_bases
TGATCCGCGGCCGGCATGTCGTATCGGTGCCACGCCCAGCGTGGCGAGCCCGTTAAACTGCCGCCGTTTTTTGGCCTTCA
ATTTTTCGAATTTCAGCGAC

Downstream 100 bases:

>100_bases
GCGGCGCTGCGCGGTCATGGCTGGCATGCCCGTCGATAGTGCTGCGTTCGCGGCCTTCGACATGCAGGAACTCACGGCAG
CCACCTTCGACGCCAGCCTC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MENTQENPTSQNPTPADEAARQAAEAASGEPQDQARQPAAAAGEQPAQAQPAGAEAALAEAQAKLAELQESFLRAKAETE
NVRRRGQEDVAKAHKFAIESFAEHLLPVMDSLEAAVAHSTDDLAKVREGVELTLRQLTGALEKGKVVALNPVGEKFDPHR
HQAISMVPADQEPNTVVAVLQKGYVIADRVLRPALVTVAAPK

Sequences:

>Translated_202_residues
MENTQENPTSQNPTPADEAARQAAEAASGEPQDQARQPAAAAGEQPAQAQPAGAEAALAEAQAKLAELQESFLRAKAETE
NVRRRGQEDVAKAHKFAIESFAEHLLPVMDSLEAAVAHSTDDLAKVREGVELTLRQLTGALEKGKVVALNPVGEKFDPHR
HQAISMVPADQEPNTVVAVLQKGYVIADRVLRPALVTVAAPK
>Mature_202_residues
MENTQENPTSQNPTPADEAARQAAEAASGEPQDQARQPAAAAGEQPAQAQPAGAEAALAEAQAKLAELQESFLRAKAETE
NVRRRGQEDVAKAHKFAIESFAEHLLPVMDSLEAAVAHSTDDLAKVREGVELTLRQLTGALEKGKVVALNPVGEKFDPHR
HQAISMVPADQEPNTVVAVLQKGYVIADRVLRPALVTVAAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=170, Percent_Identity=37.6470588235294, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI40255109, Length=202, Percent_Identity=34.1584158415842, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788967, Length=204, Percent_Identity=37.7450980392157, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17552458, Length=177, Percent_Identity=34.4632768361582, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=34.375, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653432, Length=179, Percent_Identity=32.9608938547486, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BURP8 (B2JGE4)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858718.1
- GeneID:   6243983
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2499
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14139
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21473; Mature: 21473

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPTSQNPTPADEAARQAAEAASGEPQDQARQPAAAAGEQPAQAQPAGAEAALAE
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCCCCCCCHHHHHHH
AQAKLAELQESFLRAKAETENVRRRGQEDVAKAHKFAIESFAEHLLPVMDSLEAAVAHST
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DDLAKVREGVELTLRQLTGALEKGKVVALNPVGEKFDPHRHQAISMVPADQEPNTVVAVL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHH
QKGYVIADRVLRPALVTVAAPK
HCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENTQENPTSQNPTPADEAARQAAEAASGEPQDQARQPAAAAGEQPAQAQPAGAEAALAE
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCCCCCCCHHHHHHH
AQAKLAELQESFLRAKAETENVRRRGQEDVAKAHKFAIESFAEHLLPVMDSLEAAVAHST
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DDLAKVREGVELTLRQLTGALEKGKVVALNPVGEKFDPHRHQAISMVPADQEPNTVVAVL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHH
QKGYVIADRVLRPALVTVAAPK
HCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA