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The map label for this gene is mscS [H]

Identifier: 186477232

GI number: 186477232

Start: 2792166

End: 2792978

Strand: Direct

Name: mscS [H]

Synonym: Bphy_2483

Alternate gene names: 186477232

Gene position: 2792166-2792978 (Clockwise)

Preceding gene: 186477231

Following gene: 186477234

Centisome position: 80.25

GC content: 63.71

Gene sequence:

>813_bases
GTGGATATCGAAGCCGTACGCGTCTTTCTGATGACCCGGGGAATCGACTTCGGGACCAAGGTACTCGGTGCAATCGTTCT
GTGGCTCATCGGGCGATGGGCGATCGGCCTCATCATCGGCCTGATGCGCAAAGTGCTCGCGCGCAACAGCAGGGTCGATC
CGACACTCGCGCACTATCTCGGCTCGATCCTCGCCGGCATTCTGAACCTGCTGCTGGTGCTCGCGATCCTGCAGGTGTTC
GGCGTGCAGACCACGTCGTTCGCCGCGCTGCTCGCGGGCGTCGGCCTCGCGGTCGGAACGGCATGGGGCGGGCTGCTCGC
GCACTTCGCGGCAGGCATCTTCATGCAGGTGCTGCGGCCGTTCAAGGTCGGCGATTTCGTGACAGCGGGCGGCGTGACGG
GCACGGTCCAGGAACTCGGTCTGTTCGGCACGACGATCGTCACGCCCGACAATGTGTTGACCATCGTCGGCAACAACACG
GTTTTCTCCGGGGTGATCTCGAATTACAGCGCGCAACCGGTGCGGCGCGTCGAACTGACGGCGAAGGTTGCGAACGGCGT
GGACCCGATCGATGCGGCAAACCGGCTGCGCGCCGCCGTCACGAAGATTCCGAATGTTGCGGAGAATCCGCCGCCCGATA
TCGAGGTGTTGTCGTTCACGCCGGAAGGACCGCTCCTGTGCGTTCGGCCTTACACGCATAACGACAACTACTGGCAGGTG
TACTTCGATACGAACCGCGCGATCATCGATACCTTTCGCGAAGCGGGCTATCCGACGCCGGAAACCCCCGTCGTGCGGCG
CGCGGCGACCTGA

Upstream 100 bases:

>100_bases
ACGCGATGGACGCGGCGTCCGCTTTCCCGATCCGCCTCGCGTCCGCTGCGTCGTCGCATGCATGAGTCAGGCATGCGCAA
CCGACTCAAGCGAGGGAATC

Downstream 100 bases:

>100_bases
GGTTTGTTCTTCTCGATTCGTTGCGCGGGCATCCGTCGATTGCGTCGAACGAATGCCCGTGACTTGCGTGGGTCAACGGC
CCGTGTGATTGCCCGCCGAG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDIEAVRVFLMTRGIDFGTKVLGAIVLWLIGRWAIGLIIGLMRKVLARNSRVDPTLAHYLGSILAGILNLLLVLAILQVF
GVQTTSFAALLAGVGLAVGTAWGGLLAHFAAGIFMQVLRPFKVGDFVTAGGVTGTVQELGLFGTTIVTPDNVLTIVGNNT
VFSGVISNYSAQPVRRVELTAKVANGVDPIDAANRLRAAVTKIPNVAENPPPDIEVLSFTPEGPLLCVRPYTHNDNYWQV
YFDTNRAIIDTFREAGYPTPETPVVRRAAT

Sequences:

>Translated_270_residues
MDIEAVRVFLMTRGIDFGTKVLGAIVLWLIGRWAIGLIIGLMRKVLARNSRVDPTLAHYLGSILAGILNLLLVLAILQVF
GVQTTSFAALLAGVGLAVGTAWGGLLAHFAAGIFMQVLRPFKVGDFVTAGGVTGTVQELGLFGTTIVTPDNVLTIVGNNT
VFSGVISNYSAQPVRRVELTAKVANGVDPIDAANRLRAAVTKIPNVAENPPPDIEVLSFTPEGPLLCVRPYTHNDNYWQV
YFDTNRAIIDTFREAGYPTPETPVVRRAAT
>Mature_270_residues
MDIEAVRVFLMTRGIDFGTKVLGAIVLWLIGRWAIGLIIGLMRKVLARNSRVDPTLAHYLGSILAGILNLLLVLAILQVF
GVQTTSFAALLAGVGLAVGTAWGGLLAHFAAGIFMQVLRPFKVGDFVTAGGVTGTVQELGLFGTTIVTPDNVLTIVGNNT
VFSGVISNYSAQPVRRVELTAKVANGVDPIDAANRLRAAVTKIPNVAENPPPDIEVLSFTPEGPLLCVRPYTHNDNYWQV
YFDTNRAIIDTFREAGYPTPETPVVRRAAT

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=239, Percent_Identity=31.7991631799163, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI2367355, Length=195, Percent_Identity=24.1025641025641, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786670, Length=201, Percent_Identity=26.3681592039801, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 28968; Mature: 28968

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEAVRVFLMTRGIDFGTKVLGAIVLWLIGRWAIGLIIGLMRKVLARNSRVDPTLAHYL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GSILAGILNLLLVLAILQVFGVQTTSFAALLAGVGLAVGTAWGGLLAHFAAGIFMQVLRP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKVGDFVTAGGVTGTVQELGLFGTTIVTPDNVLTIVGNNTVFSGVISNYSAQPVRRVELT
CCCCCEEECCCCCHHHHHHHHHCCEEECCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHH
AKVANGVDPIDAANRLRAAVTKIPNVAENPPPDIEVLSFTPEGPLLCVRPYTHNDNYWQV
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEE
YFDTNRAIIDTFREAGYPTPETPVVRRAAT
EEECCHHHHHHHHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MDIEAVRVFLMTRGIDFGTKVLGAIVLWLIGRWAIGLIIGLMRKVLARNSRVDPTLAHYL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GSILAGILNLLLVLAILQVFGVQTTSFAALLAGVGLAVGTAWGGLLAHFAAGIFMQVLRP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKVGDFVTAGGVTGTVQELGLFGTTIVTPDNVLTIVGNNTVFSGVISNYSAQPVRRVELT
CCCCCEEECCCCCHHHHHHHHHCCEEECCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHH
AKVANGVDPIDAANRLRAAVTKIPNVAENPPPDIEVLSFTPEGPLLCVRPYTHNDNYWQV
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEE
YFDTNRAIIDTFREAGYPTPETPVVRRAAT
EEECCHHHHHHHHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]