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The map label for this gene is mdh [H]

Identifier: 186477144

GI number: 186477144

Start: 2687715

End: 2688779

Strand: Reverse

Name: mdh [H]

Synonym: Bphy_2393

Alternate gene names: 186477144

Gene position: 2688779-2687715 (Counterclockwise)

Preceding gene: 186477146

Following gene: 186477142

Centisome position: 77.28

GC content: 64.32

Gene sequence:

>1065_bases
ATGAAGACACAAATCCGATACTCCGCCGATGAACTGCAAGTCTTCGCACAGCGTCTACTGGTCAGGGTTGGAATGGAGGA
GGTCAAAGCGCTCGCAATCGCTCGCACGCTCGTCGACGGCGACCTGATGGGTCACGACACGCACGGCCTCGCGTTGCTGC
CCGCCTATATCGGTGAGATCGAAAACGGCGCGATGACCTGCTCCGGCGAGCCGGAGGTCGTATCAGACCGGGGCGGCAGC
GTGTTGTGGGATGGGCGGCGTCTTCCTGGCCCCTGGCTGGTTCTCAGCGGGATCGAAACACTGGCACCGCGTGCGAAGAA
GTACGGCACGGCGACATTGGTGATTCGGCGCAACCACCACATCGCCTGCCTTGCCAGTTATCTCGAAAGAGCCACTGCGG
ACGGATTGATGATCTTGCTCAGCAGTTCCGACCCGGCGGGGCAAAGCGTGGCCCCTCACGGCGGCACGCGGTCCGTATTT
ACACCGAATCCGATCGCGGCGGGCATTCCGACCTCGGGCACACCGTTTTTGATCGATATCTCTTCATCAATGGTGACGCA
GGGGCTGACCGCGCGCCGACACAAAGCGGGACAACACTTCGACGAAGCATGCTTCCTCGATGCGGACGGGCAGCCCAGCG
GCGATCCCGGTGTGCTCTGCACGGATCCCCCGGGATCCATCCTGCCGATCGGGGGCATGACGGGGGGACATAAAGGCTTC
GGGCTGGCGTTGCTGATCGAGGCGTTGACGGGGGGGCTGGCAGGCCACGGCCGGGCCGATCCTCCCGATGGATGGGGCGC
TACAGTGTTTCTTTCGCTCTACGATCCGGCGGCTTTTGGCGGTCTCGCGGCGTTCGTGCGGCAGATGGACTGGCTCGGCG
ACGCATGCCGCGATAATCCGCCGCGCCCAGGAACCGCGGGCGTGCGGATGCCGGGCGATCGCGGACTGAACCTCAAGCGC
GAGCAACTGCGCGATGGAGTCGCTCTGCACTCGACAATCGCGCCGGCACTTGAAGCCTGCGCGCGGCGCTATGACATCCC
GCTGCCTTCCGCACTACCCGGCTGA

Upstream 100 bases:

>100_bases
GCAATTGGACGGTTATCGCAAGCTCGAACGACAATTCAAAGCGCGTCGGGCATTGCCGGTCCGACTTTCTTCGAACAATC
TTCCTCACTGACGCCCACCC

Downstream 100 bases:

>100_bases
CAGCAGGCGCGAGAGCCCAGACTCTCGCCGAGGCAGCATTGTTTTGCTGTTACACGTCGCGAGTGAGCGCGACCGGCGGA
ATCGGGCCGCGCACGAGCAA

Product: malate/L-lactate dehydrogenase

Products: NA

Alternate protein names: (R)-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKTQIRYSADELQVFAQRLLVRVGMEEVKALAIARTLVDGDLMGHDTHGLALLPAYIGEIENGAMTCSGEPEVVSDRGGS
VLWDGRRLPGPWLVLSGIETLAPRAKKYGTATLVIRRNHHIACLASYLERATADGLMILLSSSDPAGQSVAPHGGTRSVF
TPNPIAAGIPTSGTPFLIDISSSMVTQGLTARRHKAGQHFDEACFLDADGQPSGDPGVLCTDPPGSILPIGGMTGGHKGF
GLALLIEALTGGLAGHGRADPPDGWGATVFLSLYDPAAFGGLAAFVRQMDWLGDACRDNPPRPGTAGVRMPGDRGLNLKR
EQLRDGVALHSTIAPALEACARRYDIPLPSALPG

Sequences:

>Translated_354_residues
MKTQIRYSADELQVFAQRLLVRVGMEEVKALAIARTLVDGDLMGHDTHGLALLPAYIGEIENGAMTCSGEPEVVSDRGGS
VLWDGRRLPGPWLVLSGIETLAPRAKKYGTATLVIRRNHHIACLASYLERATADGLMILLSSSDPAGQSVAPHGGTRSVF
TPNPIAAGIPTSGTPFLIDISSSMVTQGLTARRHKAGQHFDEACFLDADGQPSGDPGVLCTDPPGSILPIGGMTGGHKGF
GLALLIEALTGGLAGHGRADPPDGWGATVFLSLYDPAAFGGLAAFVRQMDWLGDACRDNPPRPGTAGVRMPGDRGLNLKR
EQLRDGVALHSTIAPALEACARRYDIPLPSALPG
>Mature_354_residues
MKTQIRYSADELQVFAQRLLVRVGMEEVKALAIARTLVDGDLMGHDTHGLALLPAYIGEIENGAMTCSGEPEVVSDRGGS
VLWDGRRLPGPWLVLSGIETLAPRAKKYGTATLVIRRNHHIACLASYLERATADGLMILLSSSDPAGQSVAPHGGTRSVF
TPNPIAAGIPTSGTPFLIDISSSMVTQGLTARRHKAGQHFDEACFLDADGQPSGDPGVLCTDPPGSILPIGGMTGGHKGF
GLALLIEALTGGLAGHGRADPPDGWGATVFLSLYDPAAFGGLAAFVRQMDWLGDACRDNPPRPGTAGVRMPGDRGLNLKR
EQLRDGVALHSTIAPALEACARRYDIPLPSALPG

Specific function: Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787020, Length=360, Percent_Identity=26.6666666666667, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790000, Length=341, Percent_Identity=22.5806451612903, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786727, Length=345, Percent_Identity=23.4782608695652, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507179, Length=327, Percent_Identity=25.9938837920489, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17536633, Length=285, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24658010, Length=317, Percent_Identity=25.5520504731861, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45553211, Length=259, Percent_Identity=23.1660231660232, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667912, Length=259, Percent_Identity=23.1660231660232, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667908, Length=259, Percent_Identity=23.1660231660232, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667904, Length=318, Percent_Identity=23.2704402515723, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648360, Length=349, Percent_Identity=23.4957020057307, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.272; =1.1.1.37; =1.1.1.82 [H]

Molecular weight: Translated: 37134; Mature: 37134

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQIRYSADELQVFAQRLLVRVGMEEVKALAIARTLVDGDLMGHDTHGLALLPAYIGEI
CCCCEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHC
ENGAMTCSGEPEVVSDRGGSVLWDGRRLPGPWLVLSGIETLAPRAKKYGTATLVIRRNHH
CCCEEEECCCCCEECCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCC
IACLASYLERATADGLMILLSSSDPAGQSVAPHGGTRSVFTPNPIAAGIPTSGTPFLIDI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
SSSMVTQGLTARRHKAGQHFDEACFLDADGQPSGDPGVLCTDPPGSILPIGGMTGGHKGF
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCH
GLALLIEALTGGLAGHGRADPPDGWGATVFLSLYDPAAFGGLAAFVRQMDWLGDACRDNP
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
PRPGTAGVRMPGDRGLNLKREQLRDGVALHSTIAPALEACARRYDIPLPSALPG
CCCCCCCEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKTQIRYSADELQVFAQRLLVRVGMEEVKALAIARTLVDGDLMGHDTHGLALLPAYIGEI
CCCCEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHC
ENGAMTCSGEPEVVSDRGGSVLWDGRRLPGPWLVLSGIETLAPRAKKYGTATLVIRRNHH
CCCEEEECCCCCEECCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCC
IACLASYLERATADGLMILLSSSDPAGQSVAPHGGTRSVFTPNPIAAGIPTSGTPFLIDI
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
SSSMVTQGLTARRHKAGQHFDEACFLDADGQPSGDPGVLCTDPPGSILPIGGMTGGHKGF
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCH
GLALLIEALTGGLAGHGRADPPDGWGATVFLSLYDPAAFGGLAAFVRQMDWLGDACRDNP
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
PRPGTAGVRMPGDRGLNLKREQLRDGVALHSTIAPALEACARRYDIPLPSALPG
CCCCCCCEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110059; 10850983 [H]