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The map label for this gene is glnH [H]

Identifier: 186477120

GI number: 186477120

Start: 2661206

End: 2661976

Strand: Reverse

Name: glnH [H]

Synonym: Bphy_2369

Alternate gene names: 186477120

Gene position: 2661976-2661206 (Counterclockwise)

Preceding gene: 186477122

Following gene: 186477119

Centisome position: 76.51

GC content: 60.31

Gene sequence:

>771_bases
ATGGATACGTTCGTCAAACGCTTCACCCTGTTCGCAGCTGGTCTTGCCGCAGCTTTCGCGATTTCATCCGGTGCCGCAAA
TGCCGCCGAACCGACCTACAAGGTCGGCGCAACGGCCACGGGCGTGCCGTTCACGTTTCTCGACGTCAAGACGAATTCAA
TCCAGGGCATGATGGTCGACGCGATCACCGCCGCGGGCAAAGCGGCGGGCTTCAACGTCGACGTGCAGCAGAATGTGTTC
TCGGCGCTGATTCCGTCGCTGACCACTCAGAAGATCGACATCATTTCGGCTGCGATGCTCAAGACGCCAGCACGCCAGCA
GATCGTCGATTTCTCGGACACCGTCTATTCGTACGGCGAAGGTGTCGTCGTGAAAGCGGACGACAAGGGCCACTACACGT
CGATGGATGACTTCAAGGGCGAGGTAGTGGGCGCGCAGGTCGGGACCGCGTTCGTCGATGCGCTCAACAAGAAAGGCATC
TTCAAGGAAGTGCGCACCTACGATTCGGTCGCGGACATCATGCGCGACGTCGCGCTCGGGCGCATCAAGGCTGGCTTCGC
GGATCAGCCCATTGTCGCGTATCAACTGCAGCAGGGCGCCAATGCGCAGGTCCGGCTCGTGCCCGAATACCAGTCGGCAG
TGAAGGGACAGGTGTGCTTCATCGTGCGCAAGGGCGACACCGCAACGCTCGAGCAGCTTAATGCCGCGATCAGGAAGATG
AAGGCCGACGGCACGCTGCAGCAGATTCTGCAGAAGTGGCACATGGGCTGA

Upstream 100 bases:

>100_bases
TCTCGTGGCGATTTTTTTGATTCGATTATGATGGTCCGGCGGTCACAACATGAAATCAGCCGGCGCGTGTTGACACGCCG
GCTCCTGCCCGGAGATATGC

Downstream 100 bases:

>100_bases
CAGGGCAGCCGTATCGCTGCATTCGAAACTGTCCGCGTGTGAACGCGCGCGGACGATTTGCCATGCGTGTCGGGAGCCGC
GATGTTTTTGCAGAACGCAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDTFVKRFTLFAAGLAAAFAISSGAANAAEPTYKVGATATGVPFTFLDVKTNSIQGMMVDAITAAGKAAGFNVDVQQNVF
SALIPSLTTQKIDIISAAMLKTPARQQIVDFSDTVYSYGEGVVVKADDKGHYTSMDDFKGEVVGAQVGTAFVDALNKKGI
FKEVRTYDSVADIMRDVALGRIKAGFADQPIVAYQLQQGANAQVRLVPEYQSAVKGQVCFIVRKGDTATLEQLNAAIRKM
KADGTLQQILQKWHMG

Sequences:

>Translated_256_residues
MDTFVKRFTLFAAGLAAAFAISSGAANAAEPTYKVGATATGVPFTFLDVKTNSIQGMMVDAITAAGKAAGFNVDVQQNVF
SALIPSLTTQKIDIISAAMLKTPARQQIVDFSDTVYSYGEGVVVKADDKGHYTSMDDFKGEVVGAQVGTAFVDALNKKGI
FKEVRTYDSVADIMRDVALGRIKAGFADQPIVAYQLQQGANAQVRLVPEYQSAVKGQVCFIVRKGDTATLEQLNAAIRKM
KADGTLQQILQKWHMG
>Mature_256_residues
MDTFVKRFTLFAAGLAAAFAISSGAANAAEPTYKVGATATGVPFTFLDVKTNSIQGMMVDAITAAGKAAGFNVDVQQNVF
SALIPSLTTQKIDIISAAMLKTPARQQIVDFSDTVYSYGEGVVVKADDKGHYTSMDDFKGEVVGAQVGTAFVDALNKKGI
FKEVRTYDSVADIMRDVALGRIKAGFADQPIVAYQLQQGANAQVRLVPEYQSAVKGQVCFIVRKGDTATLEQLNAAIRKM
KADGTLQQILQKWHMG

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=28.6307053941909, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787088, Length=244, Percent_Identity=30.327868852459, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=32.2448979591837, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788228, Length=247, Percent_Identity=28.3400809716599, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=29.6610169491525, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=26.0700389105058, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27390; Mature: 27390

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTFVKRFTLFAAGLAAAFAISSGAANAAEPTYKVGATATGVPFTFLDVKTNSIQGMMVD
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCHHHHH
AITAAGKAAGFNVDVQQNVFSALIPSLTTQKIDIISAAMLKTPARQQIVDFSDTVYSYGE
HHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GVVVKADDKGHYTSMDDFKGEVVGAQVGTAFVDALNKKGIFKEVRTYDSVADIMRDVALG
EEEEEECCCCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RIKAGFADQPIVAYQLQQGANAQVRLVPEYQSAVKGQVCFIVRKGDTATLEQLNAAIRKM
HHHCCCCCCCEEEEEECCCCCCEEEEEECHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
KADGTLQQILQKWHMG
CCCCHHHHHHHHHCCC
>Mature Secondary Structure
MDTFVKRFTLFAAGLAAAFAISSGAANAAEPTYKVGATATGVPFTFLDVKTNSIQGMMVD
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCHHHHH
AITAAGKAAGFNVDVQQNVFSALIPSLTTQKIDIISAAMLKTPARQQIVDFSDTVYSYGE
HHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GVVVKADDKGHYTSMDDFKGEVVGAQVGTAFVDALNKKGIFKEVRTYDSVADIMRDVALG
EEEEEECCCCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RIKAGFADQPIVAYQLQQGANAQVRLVPEYQSAVKGQVCFIVRKGDTATLEQLNAAIRKM
HHHCCCCCCCEEEEEECCCCCCEEEEEECHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
KADGTLQQILQKWHMG
CCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]