Click here to switch to the map view.

The map label for this gene is xylF [H]

Identifier: 186477092

GI number: 186477092

Start: 2626551

End: 2627579

Strand: Reverse

Name: xylF [H]

Synonym: Bphy_2341

Alternate gene names: 186477092

Gene position: 2627579-2626551 (Counterclockwise)

Preceding gene: 186477093

Following gene: 186477091

Centisome position: 75.52

GC content: 61.52

Gene sequence:

>1029_bases
ATGAAATCTGCAATGCGTCGTTCCGTATTGAGTTCCCTTGTTTGCGCCGCGATGGCCGCCGGCCTGACGATGATCGCGCC
GCTCGCGCATGCGAGCAAGGACAAGCCCGAGATCGGCTTCTGCATCGACGATCTGCGCGTCGAGCGCTGGTCGCGCGATC
GCGACTATTTCGTTGCGGCAGCGACGAAGCTGGGCGCGAAAGTCTCCGTGCAATCGGCAGATGCCAGCGAAGCGCGGCAA
ATTTCGCAGATCGAAAATCTGATTTCGCGCGGCGTCGATGTGATCGTGATCGTGCCGTTCAATTCGAAGACGCTCGGCAA
TGTCGTCGCCGAAGCGCACAAGGCGGGCATCAAGGTGGTGTCGTATGACCGCCTGATTCTCGATGCCGACGTCGACGCCT
ATATCTCGTTCGACAACGAGAAGGTCGGCGAACTGCAGGCGCAGGGCGTCTATAACGCACAGCCGAAAGGCAACTACTTC
CTGCTCGGCGGCGCGCCGACCGATAACAACGCGAAGATGCTGCGTGAAGGGCAGATGAAAGTGCTGAAGCCCGCGATCGA
TCGCGGCGACGTGAAGATCGTCGGTCAGCAGTGGGTGCCGGAGTGGAGCGCGGCGACGGCGCTGCGCATCACGGAAGATG
CGCTGACTGCGAACAACAACAAGATCGACGCGATCGTCGCGTCGAACGACGGCACGGCAGGCGGCGCGATTCAGGCGCTG
GCCGCACAGAAGATGGCGGGCAAGGTGCCCGTGTCCGGCCAGGATGCGGACCTTGCTGCCGTCAAGCGTGTTATTGCGGG
CACGCAGACGATGACTGTCTACAAGCCGCTGAAGCTGATCGCAGGCGAAGCGGCCAAGCTGGCCGTCGATCTCGCGAAGG
GCGACAAGCCCGCGTACAACGCGAAGTACGACAACGGCAAGAAGCAGGTCGATACCGTCCTGCTGCAACCGACGCTGCTC
ACCAAGAGCAATGTCGACGTCGTGATCAAGGACGGCTTCTACACGCAGGCGCAACTGGCTGGCAACTGA

Upstream 100 bases:

>100_bases
TTGACGCTTGCGGACAGGTATTCGCAGCGCATCCTGAGGTTAAGACGGCGGTTCGATTTCTCGCATTCGAAACTGCACGC
AATCCTAAATGGAGTGAGAC

Downstream 100 bases:

>100_bases
TAGCGGGCACGGGCGCTTCCTCGTCTTCGTGACGGGAAGCGCCCGCGCATCTTTCATCCATCCAGGACATTACGCATGGC
ACAAGCGCTTCTGGAGCTGC

Product: D-xylose ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKSAMRRSVLSSLVCAAMAAGLTMIAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEARQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVVSYDRLILDADVDAYISFDNEKVGELQAQGVYNAQPKGNYF
LLGGAPTDNNAKMLREGQMKVLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAYNAKYDNGKKQVDTVLLQPTLL
TKSNVDVVIKDGFYTQAQLAGN

Sequences:

>Translated_342_residues
MKSAMRRSVLSSLVCAAMAAGLTMIAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEARQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVVSYDRLILDADVDAYISFDNEKVGELQAQGVYNAQPKGNYF
LLGGAPTDNNAKMLREGQMKVLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAYNAKYDNGKKQVDTVLLQPTLL
TKSNVDVVIKDGFYTQAQLAGN
>Mature_342_residues
MKSAMRRSVLSSLVCAAMAAGLTMIAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEARQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVVSYDRLILDADVDAYISFDNEKVGELQAQGVYNAQPKGNYF
LLGGAPTDNNAKMLREGQMKVLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAYNAKYDNGKKQVDTVLLQPTLL
TKSNVDVVIKDGFYTQAQLAGN

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=61.2179487179487, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790526, Length=226, Percent_Identity=30.9734513274336, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36401; Mature: 36401

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAMRRSVLSSLVCAAMAAGLTMIAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCEEEEE
ATKLGAKVSVQSADASEARQISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVV
EHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEE
SYDRLILDADVDAYISFDNEKVGELQAQGVYNAQPKGNYFLLGGAPTDNNAKMLREGQMK
EECEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCHH
VLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
HHHHCCCCCCEEEEECCCCCCCCCCEEEEEEHHHHCCCCCEEEEEEECCCCCCCHHHHHH
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAYN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHEEEEECCCCCCCCC
AKYDNGKKQVDTVLLQPTLLTKSNVDVVIKDGFYTQAQLAGN
CCCCCCHHHHHEEEECCEEEECCCCCEEEECCCEEEEEECCC
>Mature Secondary Structure
MKSAMRRSVLSSLVCAAMAAGLTMIAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCEEEEE
ATKLGAKVSVQSADASEARQISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVV
EHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEE
SYDRLILDADVDAYISFDNEKVGELQAQGVYNAQPKGNYFLLGGAPTDNNAKMLREGQMK
EECEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCHH
VLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
HHHHCCCCCCEEEEECCCCCCCCCCEEEEEEHHHHCCCCCEEEEEEECCCCCCCHHHHHH
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAYN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHEEEEECCCCCCCCC
AKYDNGKKQVDTVLLQPTLLTKSNVDVVIKDGFYTQAQLAGN
CCCCCCHHHHHEEEECCEEEECCCCCEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]