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The map label for this gene is ytcB [H]

Identifier: 186477071

GI number: 186477071

Start: 2604436

End: 2605338

Strand: Reverse

Name: ytcB [H]

Synonym: Bphy_2320

Alternate gene names: 186477071

Gene position: 2605338-2604436 (Counterclockwise)

Preceding gene: 186477073

Following gene: 186477070

Centisome position: 74.88

GC content: 62.02

Gene sequence:

>903_bases
TTGCGGCATCCGTCTCAGCCAGTCGCCCACCGCGTTCTCATCACGGGAGCGAACGGTTTTACTGGGGCCTACGTGCGCGA
AGAACTCGCCGACGCGGGCTACGAGGTCATCGGCACGACGGCGGGCGAGCCAGCGGCGGGCGAACGGAAGCTCGACATCA
CGTCCCTGCCGGATTGCCGCCGCCTGATCGGCGAGTTGAAACCGGCGTTCATAGTGCATCTGGCGGCGATCAGCTTCGTC
GCGCACGACGATCCGTTGGAAATGTACAACGTCAACGTGATTGGCACGACGAATCTGCTGCAGGCGTGCATCGACGTCGG
ACATGAACCGCGCAAGATCCTGATTGCCAGCAGCGCCAACATCTACGGCAACGCGGAAGGTGTTGTCGACGAGAACACCC
CCGCCGCGCCCGTCAACCACTACGCGGCGAGCAAGCTGGCGATGGAGCATCTGGTTCGGACGTGGTTTGACCGGCTCCCC
ATCGTTGTCACCCGGCCTTTCAACTACACGGGGCGCGGGCAGTCCGAGCGTTTCCTGGTTCCCAAAATCGTCTCTCATTT
CGTCCGTCGACGGCCCTTTATCGAGCTCGGAAACCTCAATGTCGCGCGCGATTTCTCGGATGTGCGCATGGTGTCGCAAG
CGTATCGGGCGCTGCTCGAATCGCCCGTTGCGGGCGAGACGCTCAACGTGTGCAGCGGTAGCCCGCGTACGCTCACCGAC
ATCATCGACATCGTACGCGAGGCGTCCGGCCACGATCTCGAAATTCGCGTAAATCCCGCCTTCGTCCGGCAAAACGAGGT
AAAAGTGCTGGTTGGATCGGCCAAACGGCTCAATCGCGTCGTGCCCGACATGCCTGCGATCGACCTTGAAGACACCATCG
GCTGGATGCTCTCGGTCGCCTGA

Upstream 100 bases:

>100_bases
TGAGCGGAGCCGATTCATTCGTCCGATATCAATCGGCCGTTGCGCGCTCAAGCTGCGCATCAAGGCGCGCGTCAGCGCAC
TCGGATCAGGGGGAAGTAAT

Downstream 100 bases:

>100_bases
TTTTCAAACTGCATCAGTTTGTTAGTGCGCTGGCGTTTCTCTTCGACACCTTTATAATGGCCGGTTACGTACCCACGTGC
GCCTGTGTGTGACGCCGGTC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRHPSQPVAHRVLITGANGFTGAYVREELADAGYEVIGTTAGEPAAGERKLDITSLPDCRRLIGELKPAFIVHLAAISFV
AHDDPLEMYNVNVIGTTNLLQACIDVGHEPRKILIASSANIYGNAEGVVDENTPAAPVNHYAASKLAMEHLVRTWFDRLP
IVVTRPFNYTGRGQSERFLVPKIVSHFVRRRPFIELGNLNVARDFSDVRMVSQAYRALLESPVAGETLNVCSGSPRTLTD
IIDIVREASGHDLEIRVNPAFVRQNEVKVLVGSAKRLNRVVPDMPAIDLEDTIGWMLSVA

Sequences:

>Translated_300_residues
MRHPSQPVAHRVLITGANGFTGAYVREELADAGYEVIGTTAGEPAAGERKLDITSLPDCRRLIGELKPAFIVHLAAISFV
AHDDPLEMYNVNVIGTTNLLQACIDVGHEPRKILIASSANIYGNAEGVVDENTPAAPVNHYAASKLAMEHLVRTWFDRLP
IVVTRPFNYTGRGQSERFLVPKIVSHFVRRRPFIELGNLNVARDFSDVRMVSQAYRALLESPVAGETLNVCSGSPRTLTD
IIDIVREASGHDLEIRVNPAFVRQNEVKVLVGSAKRLNRVVPDMPAIDLEDTIGWMLSVA
>Mature_300_residues
MRHPSQPVAHRVLITGANGFTGAYVREELADAGYEVIGTTAGEPAAGERKLDITSLPDCRRLIGELKPAFIVHLAAISFV
AHDDPLEMYNVNVIGTTNLLQACIDVGHEPRKILIASSANIYGNAEGVVDENTPAAPVNHYAASKLAMEHLVRTWFDRLP
IVVTRPFNYTGRGQSERFLVPKIVSHFVRRRPFIELGNLNVARDFSDVRMVSQAYRALLESPVAGETLNVCSGSPRTLTD
IIDIVREASGHDLEIRVNPAFVRQNEVKVLVGSAKRLNRVVPDMPAIDLEDTIGWMLSVA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=272, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI48994969, Length=266, Percent_Identity=29.3233082706767, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788353, Length=335, Percent_Identity=23.5820895522388, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=337, Percent_Identity=24.9258160237389, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=337, Percent_Identity=24.9258160237389, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=337, Percent_Identity=24.9258160237389, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=337, Percent_Identity=24.9258160237389, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=337, Percent_Identity=24.9258160237389, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=336, Percent_Identity=24.702380952381, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24158427, Length=333, Percent_Identity=27.6276276276276, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 32937; Mature: 32937

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHPSQPVAHRVLITGANGFTGAYVREELADAGYEVIGTTAGEPAAGERKLDITSLPDCR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCHHH
RLIGELKPAFIVHLAAISFVAHDDPLEMYNVNVIGTTNLLQACIDVGHEPRKILIASSAN
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEECCC
IYGNAEGVVDENTPAAPVNHYAASKLAMEHLVRTWFDRLPIVVTRPFNYTGRGQSERFLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEH
PKIVSHFVRRRPFIELGNLNVARDFSDVRMVSQAYRALLESPVAGETLNVCSGSPRTLTD
HHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHH
IIDIVREASGHDLEIRVNPAFVRQNEVKVLVGSAKRLNRVVPDMPAIDLEDTIGWMLSVA
HHHHHHHCCCCEEEEEECHHEEECCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHEECC
>Mature Secondary Structure
MRHPSQPVAHRVLITGANGFTGAYVREELADAGYEVIGTTAGEPAAGERKLDITSLPDCR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCHHH
RLIGELKPAFIVHLAAISFVAHDDPLEMYNVNVIGTTNLLQACIDVGHEPRKILIASSAN
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEECCC
IYGNAEGVVDENTPAAPVNHYAASKLAMEHLVRTWFDRLPIVVTRPFNYTGRGQSERFLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEH
PKIVSHFVRRRPFIELGNLNVARDFSDVRMVSQAYRALLESPVAGETLNVCSGSPRTLTD
HHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHH
IIDIVREASGHDLEIRVNPAFVRQNEVKVLVGSAKRLNRVVPDMPAIDLEDTIGWMLSVA
HHHHHHHCCCCEEEEEECHHEEECCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]