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The map label for this gene is rfbB [H]

Identifier: 186477070

GI number: 186477070

Start: 2603175

End: 2604236

Strand: Reverse

Name: rfbB [H]

Synonym: Bphy_2319

Alternate gene names: 186477070

Gene position: 2604236-2603175 (Counterclockwise)

Preceding gene: 186477071

Following gene: 186477069

Centisome position: 74.85

GC content: 60.36

Gene sequence:

>1062_bases
ATGATCCTGGTAACGGGCGGTGCCGGCTTTATCGGCGCCAATTTCGTGCTTGAATGGTTGAACACCTCGGACGAGTCGGT
CCTGAATCTGGACAAGCTCACGTACGCCGGCAATCTGGGCACGTTGAAATCGCAACAGGACAATCCCCGTCACGTGTTCG
TGCGCGCAGACATCTGCGATCGCGAAACACTCGACAAACTCTTCGCCGAACACAAGCCGCGCGCGGTGCTGCACTTCGCC
GCCGAAAGTCACGTCGACCGCTCGATCCACGGGCCGGCGGACTTCGTCCAGACCAATGTGGTCGGCACCTTCACGCTGCT
CGAAGCAGCCCGATCGTACTGGAACACGCTGAACGCTGCAGACAAAGCAGCGTTCCGCTTCCTGCACGTATCGACGGATG
AAGTGTTCGGCTCGTTGTCGGCGACCGATCCGCAATTCTCCGAAACGACACCTTACGCGCCGAACAGCCCTTATTCGGCG
ACCAAGGCAGGCTCCGATCATCTCGTGCGTGCGTATCACCACACGTATGGCTTGCCTGTGTTGACGACCAACTGCTCGAA
CAACTACGGCCCGTATCAATTCCCCGAAAAGCTCATCCCGCTGATGATCGCGAACGCGCTCGCGGCTAAGCCGCTGCCTG
TGTATGGCGACGGTCAGAACGTGCGCGACTGGCTGTACGTCGGCGACCATTGCAGCGCGATCCGCGAAGTGCTCTCGCGC
GGCGTGCCGGGCGAGACGTACAACGTCGGCGGCTGGAATGAAAAGAAGAACCTCGAAGTCGTGCACACGCTGTGCGATCT
GCTGGATCAGAAGCGTCCGAAGGCACAAGGCTCGTATCGCGAACAGATCACGTATGTGACCGATCGACCGGGCCACGACC
GCCGTTATGCGATCGATGCGCGCAAGCTTGAGCGCGAACTGGGCTGGCTGCCGGCAGAGACGTTCGAAACAGGCCTCGCG
AAGACGGTGCAGTGGTATCTCGACAACCAGGAGTGGGTCGACGAGGTCGCTTCGGGCGAGTACCGCAAGTGGGTCGAAAC
GAACTACGCGCAACGCGCGTAA

Upstream 100 bases:

>100_bases
CAGTATTGCGAGCGGCGCTGACAATTAGGTAATAATTGCCAAGCGGCTGCCGCCACGGTAGCCAGTCCACTCGGCCAAGA
ATATCTCAAGAAGGAATCCC

Downstream 100 bases:

>100_bases
GGGCGGAACGATGGCGCGCAAGGGCATTATTCTGGCGGGCGGCTCGGGCACCCGGCTATATCCTATAACGCATGTCGTGT
CGAAGCAGTTGCTTCCCGTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLEWLNTSDESVLNLDKLTYAGNLGTLKSQQDNPRHVFVRADICDRETLDKLFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARSYWNTLNAADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAAKPLPVYGDGQNVRDWLYVGDHCSAIREVLSR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDQKRPKAQGSYREQITYVTDRPGHDRRYAIDARKLERELGWLPAETFETGLA
KTVQWYLDNQEWVDEVASGEYRKWVETNYAQRA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLEWLNTSDESVLNLDKLTYAGNLGTLKSQQDNPRHVFVRADICDRETLDKLFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARSYWNTLNAADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAAKPLPVYGDGQNVRDWLYVGDHCSAIREVLSR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDQKRPKAQGSYREQITYVTDRPGHDRRYAIDARKLERELGWLPAETFETGLA
KTVQWYLDNQEWVDEVASGEYRKWVETNYAQRA
>Mature_353_residues
MILVTGGAGFIGANFVLEWLNTSDESVLNLDKLTYAGNLGTLKSQQDNPRHVFVRADICDRETLDKLFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARSYWNTLNAADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAAKPLPVYGDGQNVRDWLYVGDHCSAIREVLSR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDQKRPKAQGSYREQITYVTDRPGHDRRYAIDARKLERELGWLPAETFETGLA
KTVQWYLDNQEWVDEVASGEYRKWVETNYAQRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=35.5623100303951, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=23.8938053097345, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4504031, Length=335, Percent_Identity=25.0746268656716, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=61.8384401114206, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=62.2093023255814, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788366, Length=188, Percent_Identity=30.3191489361702, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=37.6119402985075, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=31.7507418397626, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=360, Percent_Identity=27.5, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=362, Percent_Identity=27.3480662983425, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=23.5649546827795, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=274, Percent_Identity=25.1824817518248, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=25.8953168044077, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19923002, Length=363, Percent_Identity=29.4765840220386, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=24.4712990936556, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=25.9701492537313, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39644; Mature: 39644

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLEWLNTSDESVLNLDKLTYAGNLGTLKSQQDNPRHVFVRADICD
CEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCCC
RETLDKLFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARSYWNTLNAA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAAKPLPVYGDGQNVRDWLYVGDHCSAIREVLSR
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDQKRPKAQGSYREQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCCEEEHHH
RKLERELGWLPAETFETGLAKTVQWYLDNQEWVDEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLEWLNTSDESVLNLDKLTYAGNLGTLKSQQDNPRHVFVRADICD
CEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCCC
RETLDKLFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARSYWNTLNAA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAAKPLPVYGDGQNVRDWLYVGDHCSAIREVLSR
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDQKRPKAQGSYREQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCCEEEHHH
RKLERELGWLPAETFETGLAKTVQWYLDNQEWVDEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]