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The map label for this gene is rfbD [H]

Identifier: 186477067

GI number: 186477067

Start: 2600834

End: 2601727

Strand: Reverse

Name: rfbD [H]

Synonym: Bphy_2316

Alternate gene names: 186477067

Gene position: 2601727-2600834 (Counterclockwise)

Preceding gene: 186477068

Following gene: 186477066

Centisome position: 74.78

GC content: 62.75

Gene sequence:

>894_bases
ATGAAGGCGTCTGCGCAACGGACCATCCTGCTGACGGGTGTGAACGGCCAGGTCGGATTCGAACTGGCTCGCAGCCTTCA
GGGGCTCGGCACGATCGTCGCGCCGCGTCGCGATGAACTCGATCTCTCCGACCTCGAGCAGGTTCGCCGTGTCGTGCGCG
AAGTGAAGCCCGTGCTCATCGTGAACCCGGCCGCTTACACGGCCGTCGACAAGGCCGAAACGGACGTCGACGCTGCGATG
CGGATCAACGCAGAAGCGCCATCTGTGCTTGCCGAAGAAGCGAAGCGCCTTGGCGCGGCACTCGTCCACTATTCGACGGA
CTACGTATTCGACGGCACGAAAGACGGTGCTTATGTCGAAAGCGATGCCGTCAATCCCATCAATGTCTACGGGCGCAGCA
AGCTGGAAGGCGAGCAGGCGATTGCCGCCGTCGGCGGCGCGCATATGATCTTCCGCACGAGTTGGGTGTACGGTACGCGC
GGCAAGAACTTCCTTCTGACCATGTTGCGCCTTGGCGCGGAACGCGAAGAACTGAGCGTCGTGGCCGATCAGTTCGGCGC
GCCGACATGGTCGAACACGATTGCGACGTCGACGGCGCACGTGCTAGCCCAAGCCACTGCCACCGATGGCGAGCAATGGT
GGCGCGAGCATTCGGGCGTCTACCATCTGACAGCCGGCGGCGTCACGTCGTGGTATGGCTTCGCCGAGGCGATCTTCGAG
TTCTCGAGCCTGCAAAAAAAGCCGGCCGTGAAGCCCATCCCCGCCTCCGCGTATCCCACACCGGCAAGCCGACCGACCAA
CTCGTCGATGTCGAACGACAAGCTGGCCGCTGCATTTGGTGTGCGTGCTCCCGACTGGCGTGAAGCATTGCAGCTATGCA
TGGCGGCACCTTGA

Upstream 100 bases:

>100_bases
ATCGGCATCGAATGGCCGATCGATTTCGAGCCGGTGCTGGCCGCCAAGGACGCGGCCGGAAAGCGCCTGAGCGAAGCCGA
ATGCTTTGCATGAGGGTGAT

Downstream 100 bases:

>100_bases
CGCAGACATCGGCTGCGCCCCGTGCCGGTGCGGTGGTCGTTTTTTTCAACCCGGACGCTGCGTGTATCGAACGAGCCAAC
CGGCTCGCGTCGAGGATGCC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKASAQRTILLTGVNGQVGFELARSLQGLGTIVAPRRDELDLSDLEQVRRVVREVKPVLIVNPAAYTAVDKAETDVDAAM
RINAEAPSVLAEEAKRLGAALVHYSTDYVFDGTKDGAYVESDAVNPINVYGRSKLEGEQAIAAVGGAHMIFRTSWVYGTR
GKNFLLTMLRLGAEREELSVVADQFGAPTWSNTIATSTAHVLAQATATDGEQWWREHSGVYHLTAGGVTSWYGFAEAIFE
FSSLQKKPAVKPIPASAYPTPASRPTNSSMSNDKLAAAFGVRAPDWREALQLCMAAP

Sequences:

>Translated_297_residues
MKASAQRTILLTGVNGQVGFELARSLQGLGTIVAPRRDELDLSDLEQVRRVVREVKPVLIVNPAAYTAVDKAETDVDAAM
RINAEAPSVLAEEAKRLGAALVHYSTDYVFDGTKDGAYVESDAVNPINVYGRSKLEGEQAIAAVGGAHMIFRTSWVYGTR
GKNFLLTMLRLGAEREELSVVADQFGAPTWSNTIATSTAHVLAQATATDGEQWWREHSGVYHLTAGGVTSWYGFAEAIFE
FSSLQKKPAVKPIPASAYPTPASRPTNSSMSNDKLAAAFGVRAPDWREALQLCMAAP
>Mature_297_residues
MKASAQRTILLTGVNGQVGFELARSLQGLGTIVAPRRDELDLSDLEQVRRVVREVKPVLIVNPAAYTAVDKAETDVDAAM
RINAEAPSVLAEEAKRLGAALVHYSTDYVFDGTKDGAYVESDAVNPINVYGRSKLEGEQAIAAVGGAHMIFRTSWVYGTR
GKNFLLTMLRLGAEREELSVVADQFGAPTWSNTIATSTAHVLAQATATDGEQWWREHSGVYHLTAGGVTSWYGFAEAIFE
FSSLQKKPAVKPIPASAYPTPASRPTNSSMSNDKLAAAFGVRAPDWREALQLCMAAP

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=300, Percent_Identity=29.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI33519455, Length=298, Percent_Identity=29.8657718120805, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788352, Length=288, Percent_Identity=44.7916666666667, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32029; Mature: 32029

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASAQRTILLTGVNGQVGFELARSLQGLGTIVAPRRDELDLSDLEQVRRVVREVKPVLI
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
VNPAAYTAVDKAETDVDAAMRINAEAPSVLAEEAKRLGAALVHYSTDYVFDGTKDGAYVE
ECCCHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHEECCCEEECCCCCCCEEE
SDAVNPINVYGRSKLEGEQAIAAVGGAHMIFRTSWVYGTRGKNFLLTMLRLGAEREELSV
CCCCCCEEECCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHH
VADQFGAPTWSNTIATSTAHVLAQATATDGEQWWREHSGVYHLTAGGVTSWYGFAEAIFE
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
FSSLQKKPAVKPIPASAYPTPASRPTNSSMSNDKLAAAFGVRAPDWREALQLCMAAP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKASAQRTILLTGVNGQVGFELARSLQGLGTIVAPRRDELDLSDLEQVRRVVREVKPVLI
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEE
VNPAAYTAVDKAETDVDAAMRINAEAPSVLAEEAKRLGAALVHYSTDYVFDGTKDGAYVE
ECCCHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHEECCCEEECCCCCCCEEE
SDAVNPINVYGRSKLEGEQAIAAVGGAHMIFRTSWVYGTRGKNFLLTMLRLGAEREELSV
CCCCCCEEECCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCHHHHHH
VADQFGAPTWSNTIATSTAHVLAQATATDGEQWWREHSGVYHLTAGGVTSWYGFAEAIFE
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
FSSLQKKPAVKPIPASAYPTPASRPTNSSMSNDKLAAAFGVRAPDWREALQLCMAAP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]