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The map label for this gene is rbsB [H]

Identifier: 186476993

GI number: 186476993

Start: 2515613

End: 2516578

Strand: Reverse

Name: rbsB [H]

Synonym: Bphy_2242

Alternate gene names: 186476993

Gene position: 2516578-2515613 (Counterclockwise)

Preceding gene: 186476994

Following gene: 186476992

Centisome position: 72.33

GC content: 61.9

Gene sequence:

>966_bases
ATGAACAGGATCATTCGCGCCGTCGGCGCCGGCATGCTGGCGCTAGGCATCTCGACTCTCGCTGCGACAGCGGCCCGCGC
CGACGAAAAAGTAACCGTCGGCGTCGCCATCCCCACCGCCGATCACGGTTTCACAGGCGGCATCGTCTGGTGGGCGAACA
AGGCTAAAGCCGATCTGGAAAAAGCCCACCCCGACCTGAAAGTCATCGTGAAGACAGCCGCCAATGCGCCGGAACAGGCG
AACCAGCTGCAAGACCTCGTGACCGTCAACAAGATCAACGCGCTCGTGATCTTCCCGTATGAATCGGCGTCGCTCACGCA
ACCCGTTGCGCAGGTGAAAAAGAAGGGCGTCTACGTGACGGTCGTCGATCGTGGCCTCACCGACACGAGCGCTCAGGACG
CGTACGTCGCCGGCGACAACACCGCGTTCGGCAAGATCCCCGCCGAATATCTGGCAAAAGCGCTCGACGGCAAAGGCGAC
ATCGTCGCGCTGCGCGGCATTCCCACCACGCTCGACAACGAGCGCTGGAATGCCTTCACCGGCGTGCTCAAGGCCTATCC
GAACATCAGGATCCTCGACGCGAAATACGCGAACTGGAACCGTGACGACGCCTTCAAGGTCATGCAGGACTACCTGACCC
GCTTCAAGCACATCGACGCCGTCTGGGCCGCCGACGACGACATGGCCGTCGGCGTGATGAAGGCGATCGACCAGGCCAAA
CGCAGCGACATCAAGATCGTGTTCGGCGGCGCCGGCTCGAAGGGCATGGTCAAGAACGTGATGGACGGCGCGCCGCTCAT
CAAGGCCGATGTGTCGTACTCGCCGAAGTTCATCTACGACGCGATCAAGCTGACGGCCGAGGCGCGCCTCAAAGGCGACA
AACTGCCGCCGACGACGATCATCCCGTCCGTGCTGATCACGAAGGAAAACGCGAAGGACTTCTACTTCCCGGACTCGCCG
TTCTGA

Upstream 100 bases:

>100_bases
GGGCGACGCCTGAAGTACGAAGGAACGCGCGGATGCCGGTAATAGCGTGGCAAAAACAGCAGTTCGGCAAATCATTGACG
AAAAACAGGAGACAAGAACC

Downstream 100 bases:

>100_bases
TCGCGCTCGTTGCGCTTCGCGTCGACACGCGCGAAGCGCGGCGCTTTCCCGACGTAGCCCATTCATCACATGGAGCAGCA
CGATGAAAACGATCAAAGGG

Product: ABC sugar transporter periplasmic ligand binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNRIIRAVGAGMLALGISTLAATAARADEKVTVGVAIPTADHGFTGGIVWWANKAKADLEKAHPDLKVIVKTAANAPEQA
NQLQDLVTVNKINALVIFPYESASLTQPVAQVKKKGVYVTVVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALDGKGD
IVALRGIPTTLDNERWNAFTGVLKAYPNIRILDAKYANWNRDDAFKVMQDYLTRFKHIDAVWAADDDMAVGVMKAIDQAK
RSDIKIVFGGAGSKGMVKNVMDGAPLIKADVSYSPKFIYDAIKLTAEARLKGDKLPPTTIIPSVLITKENAKDFYFPDSP
F

Sequences:

>Translated_321_residues
MNRIIRAVGAGMLALGISTLAATAARADEKVTVGVAIPTADHGFTGGIVWWANKAKADLEKAHPDLKVIVKTAANAPEQA
NQLQDLVTVNKINALVIFPYESASLTQPVAQVKKKGVYVTVVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALDGKGD
IVALRGIPTTLDNERWNAFTGVLKAYPNIRILDAKYANWNRDDAFKVMQDYLTRFKHIDAVWAADDDMAVGVMKAIDQAK
RSDIKIVFGGAGSKGMVKNVMDGAPLIKADVSYSPKFIYDAIKLTAEARLKGDKLPPTTIIPSVLITKENAKDFYFPDSP
F
>Mature_321_residues
MNRIIRAVGAGMLALGISTLAATAARADEKVTVGVAIPTADHGFTGGIVWWANKAKADLEKAHPDLKVIVKTAANAPEQA
NQLQDLVTVNKINALVIFPYESASLTQPVAQVKKKGVYVTVVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALDGKGD
IVALRGIPTTLDNERWNAFTGVLKAYPNIRILDAKYANWNRDDAFKVMQDYLTRFKHIDAVWAADDDMAVGVMKAIDQAK
RSDIKIVFGGAGSKGMVKNVMDGAPLIKADVSYSPKFIYDAIKLTAEARLKGDKLPPTTIIPSVLITKENAKDFYFPDSP
F

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=298, Percent_Identity=28.5234899328859, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790526, Length=327, Percent_Identity=27.217125382263, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790674, Length=294, Percent_Identity=25.1700680272109, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34656; Mature: 34656

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIIRAVGAGMLALGISTLAATAARADEKVTVGVAIPTADHGFTGGIVWWANKAKADLE
CCHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCCHHHHHH
KAHPDLKVIVKTAANAPEQANQLQDLVTVNKINALVIFPYESASLTQPVAQVKKKGVYVT
HHCCCCEEEEEHHCCCCHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHCCEEEE
VVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALDGKGDIVALRGIPTTLDNERWNAFT
EEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
GVLKAYPNIRILDAKYANWNRDDAFKVMQDYLTRFKHIDAVWAADDDMAVGVMKAIDQAK
HHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
RSDIKIVFGGAGSKGMVKNVMDGAPLIKADVSYSPKFIYDAIKLTAEARLKGDKLPPTTI
CCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
IPSVLITKENAKDFYFPDSPF
CCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNRIIRAVGAGMLALGISTLAATAARADEKVTVGVAIPTADHGFTGGIVWWANKAKADLE
CCHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCCHHHHHH
KAHPDLKVIVKTAANAPEQANQLQDLVTVNKINALVIFPYESASLTQPVAQVKKKGVYVT
HHCCCCEEEEEHHCCCCHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHCCEEEE
VVDRGLTDTSAQDAYVAGDNTAFGKIPAEYLAKALDGKGDIVALRGIPTTLDNERWNAFT
EEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
GVLKAYPNIRILDAKYANWNRDDAFKVMQDYLTRFKHIDAVWAADDDMAVGVMKAIDQAK
HHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
RSDIKIVFGGAGSKGMVKNVMDGAPLIKADVSYSPKFIYDAIKLTAEARLKGDKLPPTTI
CCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
IPSVLITKENAKDFYFPDSPF
CCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]