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The map label for this gene is ilvD2 [H]

Identifier: 186476972

GI number: 186476972

Start: 2493750

End: 2495423

Strand: Direct

Name: ilvD2 [H]

Synonym: Bphy_2221

Alternate gene names: 186476972

Gene position: 2493750-2495423 (Clockwise)

Preceding gene: 186476969

Following gene: 186476973

Centisome position: 71.68

GC content: 64.04

Gene sequence:

>1674_bases
ATGCCGTACAACCGTCGTTCGAAGAACATCACGCAAGGCGTCGCGCGTTCGCCGAACCGCTCGATGTATTACGCGCTCGG
CTACCAGAAGGCAGACTTCGACAAGCCGATGATCGGCATCGCCAACGGCCACTCGACCATCACGCCGTGCAACGCGGGCC
TGCAACGACTCGCCGACGCCGCCGTCGAAGCCGTCAAGCGCAGCGATGCGAATCCGCAGACCTTCGGTACGCCGACCATT
TCGGACGGCATGTCGATGGGCACGGAGGGCATGAAGTACTCGCTCGTGTCGCGCGAAGTGATCGCCGACTGCATCGAGAC
CTGCGTGCAGGGGCAATGGATGGACGGCGTCGTCGTGATCGGCGGCTGCGACAAGAACATGCCGGGCGGCATGATCGGCC
TCGCGCGAATGAACGTGCCAGGCATCTACGTGTACGGCGGGACCATCAAGCCGGGCAACTGGAAAGGCACCGACCTGACT
ATCGTGTCGTCGTTCGAGGCAGTGGGCGAATTCACGGCGGGCCGCATGTCGCAAGAAGACTTCGAAGGGGTCGAACAGAA
CGCCTGCCCGAGCACGGGCTCGTGCGGCGGCATGTACACGGCCAACACGATGAGCTCGTCGTTCGAAGCGCTCGGTATGT
CGCTGCTGTACTCGTCGACAATGGCGAACCCGGATCAGGAGAAAGTGGATTCGGCGGCCGAGTCGGCGCGCGTGCTGGTC
GAAGCCGTCAAGCAGGATCTCAAGCCGCGCGACATCATTACGAAGAAGTCGATCGAAAACGCCGTTGCGCTGATCATGGC
GACGGGCGGCTCGACGAACGCCGTGCTGCACTATCTGGCCATTGCGCACGCGGCGGAAGTGGACTGGTCCATCGAAGATT
TCGAGCGCATGCGCAAGAAAGTGCCCGTCATCTGCGATCTCAAGCCGTCGGGCAAGTATGTCGCCACCGATCTGCACAAG
GCCGGCGGCATCCCGCAGGTGCTGAAGCTGCTGCTCGATGCAGGCCTGCTGCACGGCGACTGCATCACGATCACGGGCAA
GACGATCGCCGAAGAGCTGAAGGACGTGCCAGCCAAACCGCGCGCGGACCAGAAGGTGATCTATCCGATCGACAAGGCGC
TTTACAGCGAAGGCCATCTCGCGATTCTCAAGGGCAACCTCGCGGAAGACGGCGCGGTCGCGAAGATCACCGGGCTGAAG
AACCCCGTCATCACAGGGCCCGCACGCGTGTTCGACGACGAGCAAAGTGCGATGGAAGCCATCCTCGCCGACCAGATCAA
GGCAGGCGATGTCGTCGTGCTGCGCTATCTCGGCCCGCAAGGCGGCCCCGGCATGCCGGAAATGCTGGCGCCGACCTCGG
CGATCATCGGCAAGGGTCTCGGCGAATCGGTCGGTCTTATCACGGATGGGCGCTTCTCGGGCGGCACCTGGGGCATGGTG
GTGGGTCACGTCGCGCCGGAAGCGTTCGTCGGCGGCACGATCGCACTCGTGCAGGAAGGCGATTCGATCACCATCGACGC
CCACAAGCTGCTGTTGCAACTGAACGTCGACGACGGCGAACTGCAGCGCCGCCGCGCCGCATGGAAGCAGCCCGCGCCGC
GCTATACGCGCGGCGTGCTGGCCAAGTTCGCGGCGCTCGCGAAGCCGGCCAACAAGGGCGCCGTCACCGGCTGA

Upstream 100 bases:

>100_bases
ACTAGACTGGAAAACTGCGCCCCAACCTGCGCGAGGCCGGTACGCCGCGTATGGACCACGAGCACGGCGGCGCATGACAA
AACAACTGGATGGAGTTCCC

Downstream 100 bases:

>100_bases
CGCATCGACGGGAGCGCCGCGTCGCTGACCCGCGCAGCATCGCCATCCGGCAAGAGCATGCGTCGATGGATAAAGGGGCA
CACGGATCGCCGTGTGCCCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MPYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADAAVEAVKRSDANPQTFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVIGGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLT
IVSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKKVPVICDLKPSGKYVATDLHK
AGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKPRADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMEAILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVLAKFAALAKPANKGAVTG

Sequences:

>Translated_557_residues
MPYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADAAVEAVKRSDANPQTFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVIGGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLT
IVSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKKVPVICDLKPSGKYVATDLHK
AGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKPRADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMEAILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVLAKFAALAKPANKGAVTG
>Mature_556_residues
PYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADAAVEAVKRSDANPQTFGTPTIS
DGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVIGGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLTI
VSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLVE
AVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKKVPVICDLKPSGKYVATDLHKA
GGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKPRADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLKN
PVITGPARVFDDEQSAMEAILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMVV
GHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVLAKFAALAKPANKGAVTG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=604, Percent_Identity=37.5827814569536, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1786464, Length=525, Percent_Identity=33.5238095238095, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI2367371, Length=489, Percent_Identity=33.5378323108384, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1788157, Length=469, Percent_Identity=31.1300639658849, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=45, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58993; Mature: 58862

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADA
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVEAVKRSDANPQTFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCCEEEEECHHHHHHHHHCCCCHHH
FEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
VPVICDLKPSGKYVATDLHKAGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKP
CCEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCC
RADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMEA
CCCCEEEEEHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
HHHHHCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
AKFAALAKPANKGAVTG
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADA
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AVEAVKRSDANPQTFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCCEEEEECHHHHHHHHHCCCCHHH
FEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKK
HHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
VPVICDLKPSGKYVATDLHKAGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKP
CCEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCC
RADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMEA
CCCCEEEEEHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV
HHHHHCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
AKFAALAKPANKGAVTG
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA