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The map label for this gene is cobS [H]

Identifier: 186476960

GI number: 186476960

Start: 2482812

End: 2483561

Strand: Reverse

Name: cobS [H]

Synonym: Bphy_2209

Alternate gene names: 186476960

Gene position: 2483561-2482812 (Counterclockwise)

Preceding gene: 186476961

Following gene: 186476959

Centisome position: 71.38

GC content: 63.33

Gene sequence:

>750_bases
ATGAATGAGCTGCGTTACTTTTTCACGGCGCTCGGCTACTTCACGCGCGTGCCCGTGCCGCGCTGGGTCGGCTACGAACC
GCATTATCTGAACGCGGCAGCGCGTTACTTTCCACTGGTGGGCGCGCTGGTCGGCGCGGCGGGCGCGAGTGTATACATGG
CGGCCTTGCGCATCTTTCCAGCAGGCGTCGCGGTGCTTCTGTCGATGGCCACGACGCTGCTCATCACGGGCGCATTCCAC
GAAGACGGGCTGGCCGATTGCGTCGATGCATTCGGCGGAGCGTATGCGCGCGACAACACGTTGCGCATCATGCACGACTC
GCGCATCGGCGCCTTCGGGGCGATTGCGCTCTTCGTCGCGCTCGCGCTGAAGTGGCAAACGCTCGCTACGTTGCCGCCAC
TGCGCGCGGCGTGGCTGATGATCGCGGCGCATGCCGCGAGCCGCGCATGCGCGATCAGCTATCTCGTCACGCTCGATTAC
GTGCGCGCCGAAGGCAAGGCCAGGCCCGTTGCACAACGCATGTCCCGCAACGCGTTTGGCTGCGCTATCTTTCTGGGTAT
GCCGTGGCTTTTCTGGCCGCAGTGGCGCGCCGGTTGCATCGCGCTGATCGTGCTGATTGCATTGCGCTTCATGCTGGGCC
GCTATTTCGTCAAACGCATCGGCGGCTATACGGGTGACTGCCTGGGCTTTGCGCAGCAGGTCTTCGAACTGGCCATCTAT
CTGATGGGACTCGCATGGATATCGTTCTGA

Upstream 100 bases:

>100_bases
ATGGCGAGCTTCGAGTCGGCAGGCGTCGCGCAACGCGATGCATGATGCTTGCCGGGCGCATCGAAACAGGCGGTGAAACG
ACTGCGATGAGTCAATCACG

Downstream 100 bases:

>100_bases
TCCGTCACCCGGCTGTTTCCGTGGAAGAAGGCGTGTGCTACGGGCAAAGCGACGTGGCGCTTGCCGATGCGCCCGAAGCG
TCGGCGGCCGCGCTCGCCGT

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGALVGAAGASVYMAALRIFPAGVAVLLSMATTLLITGAFH
EDGLADCVDAFGGAYARDNTLRIMHDSRIGAFGAIALFVALALKWQTLATLPPLRAAWLMIAAHAASRACAISYLVTLDY
VRAEGKARPVAQRMSRNAFGCAIFLGMPWLFWPQWRAGCIALIVLIALRFMLGRYFVKRIGGYTGDCLGFAQQVFELAIY
LMGLAWISF

Sequences:

>Translated_249_residues
MNELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGALVGAAGASVYMAALRIFPAGVAVLLSMATTLLITGAFH
EDGLADCVDAFGGAYARDNTLRIMHDSRIGAFGAIALFVALALKWQTLATLPPLRAAWLMIAAHAASRACAISYLVTLDY
VRAEGKARPVAQRMSRNAFGCAIFLGMPWLFWPQWRAGCIALIVLIALRFMLGRYFVKRIGGYTGDCLGFAQQVFELAIY
LMGLAWISF
>Mature_249_residues
MNELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGALVGAAGASVYMAALRIFPAGVAVLLSMATTLLITGAFH
EDGLADCVDAFGGAYARDNTLRIMHDSRIGAFGAIALFVALALKWQTLATLPPLRAAWLMIAAHAASRACAISYLVTLDY
VRAEGKARPVAQRMSRNAFGCAIFLGMPWLFWPQWRAGCIALIVLIALRFMLGRYFVKRIGGYTGDCLGFAQQVFELAIY
LMGLAWISF

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=33.739837398374, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27353; Mature: 27353

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGALVGAAGASVYMAALRIFP
CCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVAVLLSMATTLLITGAFHEDGLADCVDAFGGAYARDNTLRIMHDSRIGAFGAIALFVA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
LALKWQTLATLPPLRAAWLMIAAHAASRACAISYLVTLDYVRAEGKARPVAQRMSRNAFG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
CAIFLGMPWLFWPQWRAGCIALIVLIALRFMLGRYFVKRIGGYTGDCLGFAQQVFELAIY
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LMGLAWISF
HHHHHHHCC
>Mature Secondary Structure
MNELRYFFTALGYFTRVPVPRWVGYEPHYLNAAARYFPLVGALVGAAGASVYMAALRIFP
CCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVAVLLSMATTLLITGAFHEDGLADCVDAFGGAYARDNTLRIMHDSRIGAFGAIALFVA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
LALKWQTLATLPPLRAAWLMIAAHAASRACAISYLVTLDYVRAEGKARPVAQRMSRNAFG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
CAIFLGMPWLFWPQWRAGCIALIVLIALRFMLGRYFVKRIGGYTGDCLGFAQQVFELAIY
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LMGLAWISF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA