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The map label for this gene is leuA

Identifier: 186476772

GI number: 186476772

Start: 2268251

End: 2269801

Strand: Reverse

Name: leuA

Synonym: Bphy_2017

Alternate gene names: 186476772

Gene position: 2269801-2268251 (Counterclockwise)

Preceding gene: 186476773

Following gene: 186476770

Centisome position: 65.24

GC content: 61.83

Gene sequence:

>1551_bases
ATGGCAGACAAGCTCATCATTTTCGACACGACCTTGCGCGACGGCGAACAATCGCCCGGTGCGTCGATGACGAAGGAAGA
AAAGATCCGTATCGCGAAGCAACTCGAGCGGATGAAGGTCGACGTGATCGAGGCCGGCTTCGCCGCCAGCTCGAACGGCG
ACTTCGACGCGATCCACACGATCGCCGGCATGATCAAGGACAGCACCATCTGCTCGCTCGCACGCGCGAACGACAAGGAC
ATTCAGCGGGCCGCCGACGCCCTCAAGCCCGCCGACCACTTCCGCATCCACACGTTTATCGCGACGTCGCCGCTGCACAT
GGAGAAGAAGCTGCGCATGTCGCCGGAACAGGTGCTCGAGCAGGCGAAGCTGGCCGTGCGCTTCGCGCGCAAGTTCACGA
ACGACGTCGAATTCTCTCCGGAAGACGGCAGCCGATCGGACATGGACTTCCTGTGCCGCGTGCTCGAAGCGGTAATCGCG
GAAGGCGCGACGACGATCAACATCGCTGACACCGTCGGCTACGGGGTGCCGGAACTGTATGGCAATCTCGTGAAGACACT
GCGCGAACGCATCCCGAATTCGGACAAGGCCGTCTTTTCGGTGCACTGCCATAACGACCTCGGCATGGCCGTCGCGAATT
CGCTCGCGGGTGTGAAGATCGGCGGGGCGCGTCAGGTGGAATGCACCATCAACGGCCTCGGGGAGCGCGCGGGCAACACG
TCGCTCGAAGAAATCGTGATGGCTGTGAGAACCCGCAAAGATTACTTCGGCCTCGATCTTGGCATCGATACGACGCAGAT
CGTGCCGGCGTCGAAGCTCGTGTCGCAGATCACCGGCTTCGTCGTTCAGCCGAACAAGGCGGTGGTCGGCGCGAACGCGT
TTGCGCACGCGTCGGGCATCCACCAGGACGGCGTGCTCAAGGCGCGCGACACCTACGAAATCATGCGCGCGGAAGACGTG
GGCTGGACCGCGAACAAGATCGTGCTCGGCAAGCTGTCGGGCCGAAACGCGTTCAAGCAGCGGCTGCAGGAACTCGGCAT
CTCGCTCGACAGCGAGAGCGAACTGAACCTCGCGTTTCAGCGTTTCAAGGAACTCGCGGACCGCAAGGCCGAAATCTTCG
ATGAAGACATCATTGCGATCGTCACAGAAGAGTCGGCCGAAGCGCAGGAAAAAGAGCACTACAAGTTCGTGTCGCTGTCG
CAGCACTCGGAAACGGGCGAGCGTCCGCACGCGCGCATTGTCTTCTCGGTCGAAGGTAAGGAAGTGGTCGGCGAAGCGAA
CGGCAACGGCCCCGTCGACGCGACGCTGAACGCCATCGAAACGGAAGTCGGCAGCGGTTCCGAATTGCTGCTGTACTCGG
TCAACGCGATTACGACGGGCACACAGGCGCAGGGCGAAGTGACCGTGCGTCTGTCAAAGGCGGGCCGCATCGTGAACGGC
GTGGGCACGGACCCGGATATCGTTGCGGCTTCGGCGAAGGCGTATATTTCGGCGCTGAACCGTCTGTATGCGGGCGCCGA
CAAACTGAATCCCCAACGCGCGGACATCTGA

Upstream 100 bases:

>100_bases
GTCTGCCTCGTTCGTTGTTTAGCGTCGCCAGCGGTGGTGGCGCAAACACTCGAATTCCGTTTTCCAATTCCAATTGACCG
AATCCCCCTGGAGACCCGAG

Downstream 100 bases:

>100_bases
CGGCCGCCTGCTGCGCGTGGCCGCGATAAAAAACCCGCAATGGCGGGTTTTTATCGTCGTCGCCTGATGCAACAGCCGCT
TTGCGAGGCGGGTTTGCCCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHTIAGMIKDSTICSLARANDKD
IQRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLEQAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
SLEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQRFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLS
QHSETGERPHARIVFSVEGKEVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNG
VGTDPDIVAASAKAYISALNRLYAGADKLNPQRADI

Sequences:

>Translated_516_residues
MADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHTIAGMIKDSTICSLARANDKD
IQRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLEQAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
SLEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQRFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLS
QHSETGERPHARIVFSVEGKEVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNG
VGTDPDIVAASAKAYISALNRLYAGADKLNPQRADI
>Mature_515_residues
ADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHTIAGMIKDSTICSLARANDKDI
QRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLEQAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIAE
GATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNTS
LEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQRFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLSQ
HSETGERPHARIVFSVEGKEVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNGV
GTDPDIVAASAKAYISALNRLYAGADKLNPQRADI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=508, Percent_Identity=48.2283464566929, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6324682, Length=546, Percent_Identity=25.6410256410256, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=553, Percent_Identity=25.497287522604, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=386, Percent_Identity=27.720207253886, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=27.4611398963731, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BURP8 (B2JDN6)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858242.1
- ProteinModelPortal:   B2JDN6
- SMR:   B2JDN6
- GeneID:   6243506
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2017
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55874; Mature: 55743

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGMIKDSTICSLARANDKDIQRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLE
HHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHH
QAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQ
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLSQHSETGERPHARIVFSVEGK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCC
EVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNG
EEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNRLYAGADKLNPQRADI
CCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
ADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAGMIKDSTICSLARANDKDIQRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLE
HHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHH
QAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQ
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLSQHSETGERPHARIVFSVEGK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCC
EVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNG
EEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEEC
VGTDPDIVAASAKAYISALNRLYAGADKLNPQRADI
CCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA