Click here to switch to the map view.

The map label for this gene is yngJ [H]

Identifier: 186476746

GI number: 186476746

Start: 2241301

End: 2242428

Strand: Reverse

Name: yngJ [H]

Synonym: Bphy_1991

Alternate gene names: 186476746

Gene position: 2242428-2241301 (Counterclockwise)

Preceding gene: 186476747

Following gene: 186476745

Centisome position: 64.45

GC content: 65.16

Gene sequence:

>1128_bases
ATGAACTTCAATTTCACCGATGAACAGCAACAATTCGCCGACGCGCTGCGCCGTTATCTAGACAGGCACTACGCATTCGA
AGCGCGCCAGGCAATCGTGCGTTCGGAAGCAGGCGTATCGAACGAGCATTGGTCCGCGTTCGCCGAACTCGGATTGACTG
CGTTGCCCGTGCCCGAAGCACGGGGCGGCTTCGATGGCGGCACGGTCGACATGCTGGTCGTGATGCAGGAACTGGGACGC
TCACTGGTCGTCGAGCCGTACTGGTCGACGGCCGTCGGCATCGAGGCGCTGAAGCTGTCCGGTACGGGCGAGGGCGACGA
TGCGGCGTTGCTGGAACGCGCGGCGCCAGGAGAGATCAGACTGGCCGTCGCGTTTCATGAGCCGCACGCGCGCTACGACC
TGTTTTCCGTCGGCACGACAGCGACGCCTGAAGGCGACGGCTACGCATTGAGTGGAACGAAATCGGTCGTGCTGCATGGT
GCGCAGGCCGATTACTGGATCGTGCCCGCGCGGCTCGACGGAGAGATCGCGCTGTTCGTGGTCGCATGCAACGCGACAAA
CGCACAGGTCACCGATTACCGGACGATCGACGGCCAACGCGCCGCGACGCTCGTCTTCAAGGCGACGCCGGCGCGCAAGC
TGAACGGCTCACATGCAGGCGCGGCGGCGCTGGAACACATCGCCGACTACGGCATCGTGTTGCTGTGCTCGGAGGCAGTC
GGTGCGCTCGACGCACTGAATCACGCGACCGTCGAATACACGAAGACCCGCCAGCAATTCGGCGTGCCGATCGCGCGCTT
CCAGGCGCTGCAGCATCGGATGGTCGAGATGCTGATCCACGCCGAGCAGGCGCGTTCGGCCACCTATCTTGCGGCCGTGC
GCTACAGCAGCGACGACGCCGACGAGCGCCGCCGCGCGGTGTCGGCTGCCAAGGTGCGAGTGGGGCAAGCGGCGCGCTTC
GTCGGCCAGCAGGCAGTGCAGTTGCACGGCGGCATGGGCGTGACGAACGAGGTGGCGGCCGCGCACCTGTTCAAGCGTCT
CGCTATCATCGAAACCACGCTCGGCGATGTCGATCATCACCTTGCTCGGTTTGCGTCGCTGCCGGGATTTGCAACGGCCG
GCGCGTGA

Upstream 100 bases:

>100_bases
TACTACTTCAACTACCGCAAGACGTCGATCTACGGCGGCTCGAACGAAATTCAAAAGAACATCATCGCGCAGATGATTCT
CGGACTGTGAGGCGCGGCGC

Downstream 100 bases:

>100_bases
ACGCACGCACAATATATGCAAACGAGGTGAAACATGAATTTGGCCTACGAGGACATGGAAGTCGGCACGACGCACGAGGT
CGGCTCGCACACGTTCACGC

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNFNFTDEQQQFADALRRYLDRHYAFEARQAIVRSEAGVSNEHWSAFAELGLTALPVPEARGGFDGGTVDMLVVMQELGR
SLVVEPYWSTAVGIEALKLSGTGEGDDAALLERAAPGEIRLAVAFHEPHARYDLFSVGTTATPEGDGYALSGTKSVVLHG
AQADYWIVPARLDGEIALFVVACNATNAQVTDYRTIDGQRAATLVFKATPARKLNGSHAGAAALEHIADYGIVLLCSEAV
GALDALNHATVEYTKTRQQFGVPIARFQALQHRMVEMLIHAEQARSATYLAAVRYSSDDADERRRAVSAAKVRVGQAARF
VGQQAVQLHGGMGVTNEVAAAHLFKRLAIIETTLGDVDHHLARFASLPGFATAGA

Sequences:

>Translated_375_residues
MNFNFTDEQQQFADALRRYLDRHYAFEARQAIVRSEAGVSNEHWSAFAELGLTALPVPEARGGFDGGTVDMLVVMQELGR
SLVVEPYWSTAVGIEALKLSGTGEGDDAALLERAAPGEIRLAVAFHEPHARYDLFSVGTTATPEGDGYALSGTKSVVLHG
AQADYWIVPARLDGEIALFVVACNATNAQVTDYRTIDGQRAATLVFKATPARKLNGSHAGAAALEHIADYGIVLLCSEAV
GALDALNHATVEYTKTRQQFGVPIARFQALQHRMVEMLIHAEQARSATYLAAVRYSSDDADERRRAVSAAKVRVGQAARF
VGQQAVQLHGGMGVTNEVAAAHLFKRLAIIETTLGDVDHHLARFASLPGFATAGA
>Mature_375_residues
MNFNFTDEQQQFADALRRYLDRHYAFEARQAIVRSEAGVSNEHWSAFAELGLTALPVPEARGGFDGGTVDMLVVMQELGR
SLVVEPYWSTAVGIEALKLSGTGEGDDAALLERAAPGEIRLAVAFHEPHARYDLFSVGTTATPEGDGYALSGTKSVVLHG
AQADYWIVPARLDGEIALFVVACNATNAQVTDYRTIDGQRAATLVFKATPARKLNGSHAGAAALEHIADYGIVLLCSEAV
GALDALNHATVEYTKTRQQFGVPIARFQALQHRMVEMLIHAEQARSATYLAAVRYSSDDADERRRAVSAAKVRVGQAARF
VGQQAVQLHGGMGVTNEVAAAHLFKRLAIIETTLGDVDHHLARFASLPGFATAGA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=374, Percent_Identity=24.331550802139, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI187960098, Length=378, Percent_Identity=26.1904761904762, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4557231, Length=378, Percent_Identity=26.1904761904762, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4557233, Length=318, Percent_Identity=27.3584905660377, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI226958414, Length=337, Percent_Identity=24.6290801186944, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI21361497, Length=394, Percent_Identity=25.6345177664975, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4557235, Length=395, Percent_Identity=24.5569620253165, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI76496475, Length=395, Percent_Identity=24.5569620253165, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4501857, Length=376, Percent_Identity=25.531914893617, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1786223, Length=373, Percent_Identity=26.0053619302949, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=367, Percent_Identity=23.9782016348774, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17506239, Length=339, Percent_Identity=26.8436578171091, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71985184, Length=397, Percent_Identity=27.2040302267003, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=319, Percent_Identity=26.0188087774295, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355753, Length=369, Percent_Identity=24.9322493224932, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356377, Length=333, Percent_Identity=27.027027027027, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24646207, Length=321, Percent_Identity=25.8566978193146, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660351, Length=376, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363737, Length=394, Percent_Identity=22.3350253807107, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 40302; Mature: 40302

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNFTDEQQQFADALRRYLDRHYAFEARQAIVRSEAGVSNEHWSAFAELGLTALPVPEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCC
RGGFDGGTVDMLVVMQELGRSLVVEPYWSTAVGIEALKLSGTGEGDDAALLERAAPGEIR
CCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHCEEEEEECCCCCCCHHHHHHHCCCCCEE
LAVAFHEPHARYDLFSVGTTATPEGDGYALSGTKSVVLHGAQADYWIVPARLDGEIALFV
EEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCCEEEEEEEECCCEEEEE
VACNATNAQVTDYRTIDGQRAATLVFKATPARKLNGSHAGAAALEHIADYGIVLLCSEAV
EEECCCCCEECEEEECCCCCEEEEEEECCCCHHCCCCCHHHHHHHHHHHCCEEEEECHHH
GALDALNHATVEYTKTRQQFGVPIARFQALQHRMVEMLIHAEQARSATYLAAVRYSSDDA
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCH
DERRRAVSAAKVRVGQAARFVGQQAVQLHGGMGVTNEVAAAHLFKRLAIIETTLGDVDHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARFASLPGFATAGA
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFNFTDEQQQFADALRRYLDRHYAFEARQAIVRSEAGVSNEHWSAFAELGLTALPVPEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCCCC
RGGFDGGTVDMLVVMQELGRSLVVEPYWSTAVGIEALKLSGTGEGDDAALLERAAPGEIR
CCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHCEEEEEECCCCCCCHHHHHHHCCCCCEE
LAVAFHEPHARYDLFSVGTTATPEGDGYALSGTKSVVLHGAQADYWIVPARLDGEIALFV
EEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCCEEEEEEEECCCEEEEE
VACNATNAQVTDYRTIDGQRAATLVFKATPARKLNGSHAGAAALEHIADYGIVLLCSEAV
EEECCCCCEECEEEECCCCCEEEEEEECCCCHHCCCCCHHHHHHHHHHHCCEEEEECHHH
GALDALNHATVEYTKTRQQFGVPIARFQALQHRMVEMLIHAEQARSATYLAAVRYSSDDA
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCH
DERRRAVSAAKVRVGQAARFVGQQAVQLHGGMGVTNEVAAAHLFKRLAIIETTLGDVDHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARFASLPGFATAGA
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]