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The map label for this gene is yagI [H]

Identifier: 186476527

GI number: 186476527

Start: 2017481

End: 2018317

Strand: Direct

Name: yagI [H]

Synonym: Bphy_1769

Alternate gene names: 186476527

Gene position: 2017481-2018317 (Clockwise)

Preceding gene: 186476526

Following gene: 186476529

Centisome position: 57.99

GC content: 68.1

Gene sequence:

>837_bases
ATGAACCAGTCATCGACGCGCCCCGTCGCCGGCGATGCCGCGAAGGAAAAGGACGGCTCGGGCGACGAAGTCACCGCGCT
CGCCCGCGGCCTGACGGTACTGCGAAGGATAGCGGCCGCCGACGCCCCGCTGTCCAACCGCGAGCTGACCGAACTGACGG
GCATTCCGAAGCCGACCGTCTCCCGCATCACCGCGACCCTGGTCAGCGCCGGGTTCCTGTTCCGTTTGCCCGACAGCGAG
CGCTTCGTGCTTACGTCGTCGGTGCTGGAACTGAGCAACGGCTTTCTGCGCAACTTCGACATACGCGCGCGCTCGCGCCC
CTTTCTGATCGAACTGGCCGAACGCACGTCATTGTCCGTGCATCTTGCCGTGCGCGACCGGCTCGACATGGTCGTCATCG
ACGCCATCAAGCCGCGCTCCGCCGTGCTGGTGTCGCGCCTCGACGTCGGCTCGCGGATGGATCTGAGCCGCACAGCCGTC
GGCCGCGCGTATCTCGCTGCGCTCGCCGAAACGGACCGGCAAAGCCTGCTGACGGGCCTGCAGGCAGCAGCGGGCGACGA
CTGGGGAAGCATCAGCGCCCGGCTCGACGCCGCGCTGCGCGAGACGACGGCACAAGGCTTCGCCATCGCGACGGGCGAAT
GGTACGAAGGGCTCAATGCGATCGCAGCCGGCTTCATCGGGCCTTCTGGCGAGCGCTACGCGGTCAACTGCGGCGGCTCG
GCGCATCAGTGTCCGCGCGACTGGCTCATAGCGCGGGCGGCGCCCGCATTGCTCCAGACCATCGACAAGATCGTCCACGA
GATCGGCGGGACACCTGGACGCCGGCTCGACACCTGA

Upstream 100 bases:

>100_bases
GGACTTAGGCCTTTCTGAGAGAAGGTCTTAGAGCGCTCCGGATACGTCTGTGCCGCTGCGCGCGTTTCACAATATTGAAA
TAGCGTTTCATAAAAACCAC

Downstream 100 bases:

>100_bases
CATCTCATGCCGCGCTTCCAGGCCCGCCTAAAAGTGCCCACGCATCGCGCGACGGGTTCGTGACACATCGCTAATGCACG
CCTTACGGACGCGCGGATTT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNQSSTRPVAGDAAKEKDGSGDEVTALARGLTVLRRIAAADAPLSNRELTELTGIPKPTVSRITATLVSAGFLFRLPDSE
RFVLTSSVLELSNGFLRNFDIRARSRPFLIELAERTSLSVHLAVRDRLDMVVIDAIKPRSAVLVSRLDVGSRMDLSRTAV
GRAYLAALAETDRQSLLTGLQAAAGDDWGSISARLDAALRETTAQGFAIATGEWYEGLNAIAAGFIGPSGERYAVNCGGS
AHQCPRDWLIARAAPALLQTIDKIVHEIGGTPGRRLDT

Sequences:

>Translated_278_residues
MNQSSTRPVAGDAAKEKDGSGDEVTALARGLTVLRRIAAADAPLSNRELTELTGIPKPTVSRITATLVSAGFLFRLPDSE
RFVLTSSVLELSNGFLRNFDIRARSRPFLIELAERTSLSVHLAVRDRLDMVVIDAIKPRSAVLVSRLDVGSRMDLSRTAV
GRAYLAALAETDRQSLLTGLQAAAGDDWGSISARLDAALRETTAQGFAIATGEWYEGLNAIAAGFIGPSGERYAVNCGGS
AHQCPRDWLIARAAPALLQTIDKIVHEIGGTPGRRLDT
>Mature_278_residues
MNQSSTRPVAGDAAKEKDGSGDEVTALARGLTVLRRIAAADAPLSNRELTELTGIPKPTVSRITATLVSAGFLFRLPDSE
RFVLTSSVLELSNGFLRNFDIRARSRPFLIELAERTSLSVHLAVRDRLDMVVIDAIKPRSAVLVSRLDVGSRMDLSRTAV
GRAYLAALAETDRQSLLTGLQAAAGDDWGSISARLDAALRETTAQGFAIATGEWYEGLNAIAAGFIGPSGERYAVNCGGS
AHQCPRDWLIARAAPALLQTIDKIVHEIGGTPGRRLDT

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=202, Percent_Identity=26.7326732673267, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082359, Length=216, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693096, Length=219, Percent_Identity=30.5936073059361, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788581, Length=210, Percent_Identity=29.0476190476191, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29708; Mature: 29708

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSSTRPVAGDAAKEKDGSGDEVTALARGLTVLRRIAAADAPLSNRELTELTGIPKPTV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHH
SRITATLVSAGFLFRLPDSERFVLTSSVLELSNGFLRNFDIRARSRPFLIELAERTSLSV
HHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHCCHHCCCCCCCCCCCEEEEEECCCCEEE
HLAVRDRLDMVVIDAIKPRSAVLVSRLDVGSRMDLSRTAVGRAYLAALAETDRQSLLTGL
EEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAAGDDWGSISARLDAALRETTAQGFAIATGEWYEGLNAIAAGFIGPSGERYAVNCGGS
HHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AHQCPRDWLIARAAPALLQTIDKIVHEIGGTPGRRLDT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNQSSTRPVAGDAAKEKDGSGDEVTALARGLTVLRRIAAADAPLSNRELTELTGIPKPTV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHH
SRITATLVSAGFLFRLPDSERFVLTSSVLELSNGFLRNFDIRARSRPFLIELAERTSLSV
HHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHCCHHCCCCCCCCCCCEEEEEECCCCEEE
HLAVRDRLDMVVIDAIKPRSAVLVSRLDVGSRMDLSRTAVGRAYLAALAETDRQSLLTGL
EEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAAGDDWGSISARLDAALRETTAQGFAIATGEWYEGLNAIAAGFIGPSGERYAVNCGGS
HHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AHQCPRDWLIARAAPALLQTIDKIVHEIGGTPGRRLDT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]