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The map label for this gene is rbfA [H]

Identifier: 186476486

GI number: 186476486

Start: 1971081

End: 1971512

Strand: Reverse

Name: rbfA [H]

Synonym: Bphy_1728

Alternate gene names: 186476486

Gene position: 1971512-1971081 (Counterclockwise)

Preceding gene: 186476487

Following gene: 186476485

Centisome position: 56.67

GC content: 57.18

Gene sequence:

>432_bases
GTGGCTTGCTGCCGCCGATACGCGACGAACATCTTGCTATCCGGCAGCACGGATCACGGATTGACCATGCCTAAGAAACG
TACATCTCCCAATCGTAACGTGCAGATCGCCGATCAGATTCAGCGCGATCTGTCCGAACTGATTCGCGAGGTCAAAGACC
CGCGTATCGGCATTGTCACGTTCCAGAGCGTCGAACTGACGCCTGACTATGCGCACGCGAAGGTCTACTTCACGACGCTG
ACTGGCGACCCGCATCAGACGCAAGATGCGCTCAATCACGCGGCGGGCCATCTGCACAATCTCCTGTTCAAGCGACTGCA
TATCCACACGGTGCCGACGCTGCATTTTCATTACGACAAGACCATCGAGCGAGCCGTCGAGATGTCGCGTCTGATCGACG
AGGCGAATGCCTCGCGCGCAAAAGACGACTGA

Upstream 100 bases:

>100_bases
GCACATGAGGGGCGGGGCGGGCGCTGAAGCCCGGTTCCGCCCCTTTGTTTTTGTATCGACTGTTTTGTCTCGTTTGCCGC
GTTTTGTGTGGAGTTCCTTC

Downstream 100 bases:

>100_bases
CCCTGGTCGAGGGACGCGCACATATGACTCAGAACCAACGTCCCAAGGTGCCGCGTCGCGCGCTGGATGGTGTGTTGCTG
CTCGACAAGCCTGTGGGGCT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MACCRRYATNILLSGSTDHGLTMPKKRTSPNRNVQIADQIQRDLSELIREVKDPRIGIVTFQSVELTPDYAHAKVYFTTL
TGDPHQTQDALNHAAGHLHNLLFKRLHIHTVPTLHFHYDKTIERAVEMSRLIDEANASRAKDD

Sequences:

>Translated_143_residues
MACCRRYATNILLSGSTDHGLTMPKKRTSPNRNVQIADQIQRDLSELIREVKDPRIGIVTFQSVELTPDYAHAKVYFTTL
TGDPHQTQDALNHAAGHLHNLLFKRLHIHTVPTLHFHYDKTIERAVEMSRLIDEANASRAKDD
>Mature_142_residues
ACCRRYATNILLSGSTDHGLTMPKKRTSPNRNVQIADQIQRDLSELIREVKDPRIGIVTFQSVELTPDYAHAKVYFTTLT
GDPHQTQDALNHAAGHLHNLLFKRLHIHTVPTLHFHYDKTIERAVEMSRLIDEANASRAKDD

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=116, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 16282; Mature: 16150

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACCRRYATNILLSGSTDHGLTMPKKRTSPNRNVQIADQIQRDLSELIREVKDPRIGIVT
CCHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEE
FQSVELTPDYAHAKVYFTTLTGDPHQTQDALNHAAGHLHNLLFKRLHIHTVPTLHFHYDK
EEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
TIERAVEMSRLIDEANASRAKDD
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ACCRRYATNILLSGSTDHGLTMPKKRTSPNRNVQIADQIQRDLSELIREVKDPRIGIVT
CHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEE
FQSVELTPDYAHAKVYFTTLTGDPHQTQDALNHAAGHLHNLLFKRLHIHTVPTLHFHYDK
EEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
TIERAVEMSRLIDEANASRAKDD
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA