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The map label for this gene is truB [H]

Identifier: 186476485

GI number: 186476485

Start: 1970128

End: 1971057

Strand: Reverse

Name: truB [H]

Synonym: Bphy_1727

Alternate gene names: 186476485

Gene position: 1971057-1970128 (Counterclockwise)

Preceding gene: 186476486

Following gene: 186476480

Centisome position: 56.65

GC content: 62.8

Gene sequence:

>930_bases
ATGACTCAGAACCAACGTCCCAAGGTGCCGCGTCGCGCGCTGGATGGTGTGTTGCTGCTCGACAAGCCTGTGGGGCTGTC
GAGTAACGACGCGCTGATCCGCGCGAAGCGCCTGTATCTTGCGAAGAAGGCGGGGCACACGGGCACGCTCGATCCGCTCG
CGTCGGGGCTGTTGCCGCTCTGCTTCGGCGAAGCAACGAAGTTTTCGCAGGATCTGCTGGAAGCGGACAAGACGTATGAA
GCGACGATGCGGCTCGGCATTCGCACGACGACGGGCGATGCCGAAGGCGAGGCGATCGAAACGCGCGACGTGACATGCGA
TCAGGCTGCCGTCGAGCAGAGGCTCGTGCAGTTTCGCGGCGACATCGTTCAGGTGCCGCCGATGTATTCGGCGCTCAAGC
GCGGCGGCAAGCCGCTGTACGAGTACGCGCGCGCCGGGCAGACGGTCGAGCGCGAAGGGCGCCAGGTGACGATTCACGCG
CTGGAACTCATCGCGTGTGATTTGCCTGATGTGACTTTTCGTGTGACGTGCAGCAAGGGTACGTATGTTCGTACGCTGGC
CGAGGATATCGGCGAAGCGCTCGGATGCGGGGCACATCTGGTGATGTTGCGGCGTACTGGCGTCGGGGCGTTGACGCTGG
CGAATTCGGTGACGCTGGACGCGTTGTCCGATGCGGCGCAAAGCGAGCGTGATGCGTGGCTGCAGCCTGTCGATGCGTTG
CTGTCGACGTTTCCTTCCGTTCAACTGGATGAGGAAGCGACCCGGCGATTTTTGCATGGCCAACGGTTGAAGTTGACCGA
TCTTGCTGTCGACGACGGTGTTTTGCTGGCGGCTGCTCGTACGCGCGTGTATGGCAGCAAAGGGAAACTGCTTGGCGTCG
CGAAAGCCGCCGACGGTGTGCTGGCGCCCGAGCGTCTCATCGTGAGCTAG

Upstream 100 bases:

>100_bases
ACAAGACCATCGAGCGAGCCGTCGAGATGTCGCGTCTGATCGACGAGGCGAATGCCTCGCGCGCAAAAGACGACTGACCC
TGGTCGAGGGACGCGCACAT

Downstream 100 bases:

>100_bases
CGCCTGGGCGGGCGGTTTTTTGCTCCGGCATCCGTGCTTTGCCCACGGGCCTCCAACGTTCCCGCACTCCGCCCTGACAA
CGCGCGAATCCATGTGCGTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTQNQRPKVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYE
ATMRLGIRTTTGDAEGEAIETRDVTCDQAAVEQRLVQFRGDIVQVPPMYSALKRGGKPLYEYARAGQTVEREGRQVTIHA
LELIACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVMLRRTGVGALTLANSVTLDALSDAAQSERDAWLQPVDAL
LSTFPSVQLDEEATRRFLHGQRLKLTDLAVDDGVLLAAARTRVYGSKGKLLGVAKAADGVLAPERLIVS

Sequences:

>Translated_309_residues
MTQNQRPKVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYE
ATMRLGIRTTTGDAEGEAIETRDVTCDQAAVEQRLVQFRGDIVQVPPMYSALKRGGKPLYEYARAGQTVEREGRQVTIHA
LELIACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVMLRRTGVGALTLANSVTLDALSDAAQSERDAWLQPVDAL
LSTFPSVQLDEEATRRFLHGQRLKLTDLAVDDGVLLAAARTRVYGSKGKLLGVAKAADGVLAPERLIVS
>Mature_308_residues
TQNQRPKVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEA
TMRLGIRTTTGDAEGEAIETRDVTCDQAAVEQRLVQFRGDIVQVPPMYSALKRGGKPLYEYARAGQTVEREGRQVTIHAL
ELIACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVMLRRTGVGALTLANSVTLDALSDAAQSERDAWLQPVDALL
STFPSVQLDEEATRRFLHGQRLKLTDLAVDDGVLLAAARTRVYGSKGKLLGVAKAADGVLAPERLIVS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=286, Percent_Identity=32.8671328671329, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4503337, Length=300, Percent_Identity=28, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI215599015, Length=300, Percent_Identity=28, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=45.2229299363057, Blast_Score=256, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17553978, Length=292, Percent_Identity=26.7123287671233, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=199, Percent_Identity=34.1708542713568, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=264, Percent_Identity=30.3030303030303, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364189, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364187, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364185, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364183, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI62471759, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17975520, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33354; Mature: 33222

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNQRPKVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPL
CCCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCHHH
CFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIETRDVTCDQAAVEQRLVQFRG
HHCCHHHHHHHHHHHCCHHHHHHEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
DIVQVPPMYSALKRGGKPLYEYARAGQTVEREGRQVTIHALELIACDLPDVTFRVTCSKG
CEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHCCCCCCEEEEEECCC
TYVRTLAEDIGEALGCGAHLVMLRRTGVGALTLANSVTLDALSDAAQSERDAWLQPVDAL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LSTFPSVQLDEEATRRFLHGQRLKLTDLAVDDGVLLAAARTRVYGSKGKLLGVAKAADGV
HHHCCCCEECHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHEECCCCCEEEEEHHCCCC
LAPERLIVS
CCCHHHHCC
>Mature Secondary Structure 
TQNQRPKVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPL
CCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCHHH
CFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIETRDVTCDQAAVEQRLVQFRG
HHCCHHHHHHHHHHHCCHHHHHHEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
DIVQVPPMYSALKRGGKPLYEYARAGQTVEREGRQVTIHALELIACDLPDVTFRVTCSKG
CEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHCCCCCCEEEEEECCC
TYVRTLAEDIGEALGCGAHLVMLRRTGVGALTLANSVTLDALSDAAQSERDAWLQPVDAL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LSTFPSVQLDEEATRRFLHGQRLKLTDLAVDDGVLLAAARTRVYGSKGKLLGVAKAADGV
HHHCCCCEECHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHEECCCCCEEEEEHHCCCC
LAPERLIVS
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA