Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 186476454

GI number: 186476454

Start: 1930852

End: 1931595

Strand: Reverse

Name: rlmB [H]

Synonym: Bphy_1696

Alternate gene names: 186476454

Gene position: 1931595-1930852 (Counterclockwise)

Preceding gene: 186476455

Following gene: 186476452

Centisome position: 55.52

GC content: 63.71

Gene sequence:

>744_bases
ATGTCACGTCTCAAGGTTCTCTACGGTTTTCACGCGGTGACGGCACGTTTGCGCCACGATCCTTCGTCGGTCGAAGAGAT
CTATTACGACCAGACGCGCCGCGATCGCCGGATGAACGAGTTTCTTGCCGTGGCGAAAGAGGCGGGTGTACGCCTGATCG
CTGCCGACGAAACGCGTCTGTGGGGGCTGGCGCATACCGAGCGGCATCAGGGTGTGGTGGCGCGTGCGGGCGACGTGCCG
CTGGCGCAGAACCTGGCCGAACTGCTCGACGGGATTCAGGGGCCGCCATTGTTGCTCGTGCTCGATGGCATTACCGATCC
GCACAATCTCGGCGCGTGCCTGCGCGTCGCCGACGCGGCGGGCGCGCATGCGGTGATCGCGCCGCGGGATCGCGCGGTCG
GCCTGAATGCGACGGCGGCGAAGGTCGCGAGCGGGGCAGCCGATACGGTGCCGTACATTACCGTCACCAACCTGGCGCGC
GCGTTGCGCGAACTGAAGGATGCCGGCATATGGGTCGTCGGGACGGCGGGTGAGGCGACGGCATCGTTGTATGAGACGAA
GCTCGATGGTCCAGTTGCCCTTGTGATGGGCGCCGAGGGTGAGGGCATGAGAAGGCTCACGCGGGATACGTGTGACGAGG
TGATGCAGATTCCTATGGCTGGGTCGGTTGAAAGTTTGAATGTTTCCGTCGCCTCAGGGGTGTGTTTGTTTGAGGCTGTG
CGGCAGCGGGCTGTCAAGAAGTAA

Upstream 100 bases:

>100_bases
CACGCCGCGCGCACGTGCGCGGTGCTCATTCTCATCGGGCGCGCCGCGATCGGACCAGTCGATACGATCGCGGCGTGTTC
ATTCAAAGGTTGTTTCAGTC

Downstream 100 bases:

>100_bases
GCAGGCGCGTTGGCCTATGCGCTGGCATCCACGCGGGCATCCACGGTAGGCCCTCGTGCTTCAGGCGTCGCCCTTCCAGG
GCGGCGCGGGCATCACTATG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARLRHDPSSVEEIYYDQTRRDRRMNEFLAVAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDVP
LAQNLAELLDGIQGPPLLLVLDGITDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGIWVVGTAGEATASLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMQIPMAGSVESLNVSVASGVCLFEAV
RQRAVKK

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARLRHDPSSVEEIYYDQTRRDRRMNEFLAVAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDVP
LAQNLAELLDGIQGPPLLLVLDGITDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGIWVVGTAGEATASLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMQIPMAGSVESLNVSVASGVCLFEAV
RQRAVKK
>Mature_246_residues
SRLKVLYGFHAVTARLRHDPSSVEEIYYDQTRRDRRMNEFLAVAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDVPL
AQNLAELLDGIQGPPLLLVLDGITDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGIWVVGTAGEATASLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMQIPMAGSVESLNVSVASGVCLFEAVR
QRAVKK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=27.7566539923954, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=54.1322314049587, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=30.7053941908714, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=34.2105263157895, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26372; Mature: 26240

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARLRHDPSSVEEIYYDQTRRDRRMNEFLAVAKEAGVRLIAADETRL
CCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARAGDVPLAQNLAELLDGIQGPPLLLVLDGITDPHNLGACLRVADAA
EECCHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGIWVVGTAGEAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCHH
ASLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMQIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCEEHHHHHHHHHHHH
RQRAVKK
HHHHCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARLRHDPSSVEEIYYDQTRRDRRMNEFLAVAKEAGVRLIAADETRL
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARAGDVPLAQNLAELLDGIQGPPLLLVLDGITDPHNLGACLRVADAA
EECCHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGIWVVGTAGEAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCHH
ASLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMQIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCEEHHHHHHHHHHHH
RQRAVKK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]