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The map label for this gene is amiD [H]

Identifier: 186476452

GI number: 186476452

Start: 1928206

End: 1929078

Strand: Reverse

Name: amiD [H]

Synonym: Bphy_1694

Alternate gene names: 186476452

Gene position: 1929078-1928206 (Counterclockwise)

Preceding gene: 186476454

Following gene: 186476451

Centisome position: 55.45

GC content: 63.92

Gene sequence:

>873_bases
ATGAAGCCCGCCTCAAGCCGACTGGCACTGCTCGCGCTAGCAGCGACATTACTCACGACAGCCTGCGCGCCCGGCATCAT
CCACCACAACACGTATTACACAGACACGTCGCGCCCAGCGCGATACCAGGACACCCGCATCCGTTTCCTCGTGATGCACT
ACACGGAGATCGACGAAAAACAGTCGCTAGACGTACTCACGCAAGAGCAGGTCAGCGCTCACTACGTCGTCTCCGATCAT
CCAGAAGAAAAGCATGGCGAGCCCATCATCTGGCAACTGGTGCCTGAATCCCAACGCGCCTGGCACGCGGGCATCAGCTT
CTGGCAAGGCACAACGGAACTGAACGCTGCTTCGATCGGCATCGAGAACGTGAACCTCGGCCCCATCGACGGGCCGCAAG
GCCGCACATGGCAACCGTATCCGCCGCAGCAGGTCGACGCGTTGATCCGGCTCGCCAAAGACATCGTCACGCGCTACGAC
ATTCCGCCAACGCGTGTCGTCGGCCATAGCGACATCGCGCCGCAACGCAAGATCGACCCGGGCCCGGTCTTCCCGTGGAA
ACAGCTTTACGACGCAGGCGTCGGCGCGTGGCCCGACGAATCCACCGTCACGAAAGAACTCGCGGGCCGCGAGCCGCATG
CGCCCGCCGACGTTCGCGCGCTTCAGCAAAAGCTCGCGCGCTACGGCTACGAGGTGGCAACCGACGGCGTCCTCGACGAC
AGGACGCGCCGTGTGTTCGCCGCGTTCCAGATGCATTTCCGTCCCGCCGACTACTCGGGCAACCCGGATGCGCAAACGGA
TGCGATCGCCCAGGCGCTGCTCGACAAGTACGTGCCGAACGCCACACCCGAACCGGCACCCGCCGCGCCCTGA

Upstream 100 bases:

>100_bases
TTCCCGTCAGCGAATATCCGTCTCCGCCTCAGTAAGCGAAGAACAGCAGGCACCCGTCACCGCGAACAAGCCAGCAACCA
GAAGAAGAGTCCCAACCGCA

Downstream 100 bases:

>100_bases
AGCCGGCAAGGCGCCGTGCGCGCCAGATGCCGGGCCGGATGCGCAATAAACCCGGGCGAACGCCTCGACTCCGGTAAACT
TGCAGTTTTTCCGCTTGCCC

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKPASSRLALLALAATLLTTACAPGIIHHNTYYTDTSRPARYQDTRIRFLVMHYTEIDEKQSLDVLTQEQVSAHYVVSDH
PEEKHGEPIIWQLVPESQRAWHAGISFWQGTTELNAASIGIENVNLGPIDGPQGRTWQPYPPQQVDALIRLAKDIVTRYD
IPPTRVVGHSDIAPQRKIDPGPVFPWKQLYDAGVGAWPDESTVTKELAGREPHAPADVRALQQKLARYGYEVATDGVLDD
RTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYVPNATPEPAPAAP

Sequences:

>Translated_290_residues
MKPASSRLALLALAATLLTTACAPGIIHHNTYYTDTSRPARYQDTRIRFLVMHYTEIDEKQSLDVLTQEQVSAHYVVSDH
PEEKHGEPIIWQLVPESQRAWHAGISFWQGTTELNAASIGIENVNLGPIDGPQGRTWQPYPPQQVDALIRLAKDIVTRYD
IPPTRVVGHSDIAPQRKIDPGPVFPWKQLYDAGVGAWPDESTVTKELAGREPHAPADVRALQQKLARYGYEVATDGVLDD
RTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYVPNATPEPAPAAP
>Mature_290_residues
MKPASSRLALLALAATLLTTACAPGIIHHNTYYTDTSRPARYQDTRIRFLVMHYTEIDEKQSLDVLTQEQVSAHYVVSDH
PEEKHGEPIIWQLVPESQRAWHAGISFWQGTTELNAASIGIENVNLGPIDGPQGRTWQPYPPQQVDALIRLAKDIVTRYD
IPPTRVVGHSDIAPQRKIDPGPVFPWKQLYDAGVGAWPDESTVTKELAGREPHAPADVRALQQKLARYGYEVATDGVLDD
RTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYVPNATPEPAPAAP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=259, Percent_Identity=53.2818532818533, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1786300, Length=120, Percent_Identity=36.6666666666667, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32185; Mature: 32185

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASSRLALLALAATLLTTACAPGIIHHNTYYTDTSRPARYQDTRIRFLVMHYTEIDEK
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCCCCCCCCEEEEEEEEEHHCCCH
QSLDVLTQEQVSAHYVVSDHPEEKHGEPIIWQLVPESQRAWHAGISFWQGTTELNAASIG
HHHHHHHHHHHCEEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHCC
IENVNLGPIDGPQGRTWQPYPPQQVDALIRLAKDIVTRYDIPPTRVVGHSDIAPQRKIDP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
GPVFPWKQLYDAGVGAWPDESTVTKELAGREPHAPADVRALQQKLARYGYEVATDGVLDD
CCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCH
RTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYVPNATPEPAPAAP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKPASSRLALLALAATLLTTACAPGIIHHNTYYTDTSRPARYQDTRIRFLVMHYTEIDEK
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCCCCCCCCEEEEEEEEEHHCCCH
QSLDVLTQEQVSAHYVVSDHPEEKHGEPIIWQLVPESQRAWHAGISFWQGTTELNAASIG
HHHHHHHHHHHCEEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHCC
IENVNLGPIDGPQGRTWQPYPPQQVDALIRLAKDIVTRYDIPPTRVVGHSDIAPQRKIDP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
GPVFPWKQLYDAGVGAWPDESTVTKELAGREPHAPADVRALQQKLARYGYEVATDGVLDD
CCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCH
RTRRVFAAFQMHFRPADYSGNPDAQTDAIAQALLDKYVPNATPEPAPAAP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]