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The map label for this gene is hsrA [H]

Identifier: 186476397

GI number: 186476397

Start: 1860695

End: 1862137

Strand: Direct

Name: hsrA [H]

Synonym: Bphy_1639

Alternate gene names: 186476397

Gene position: 1860695-1862137 (Clockwise)

Preceding gene: 186476393

Following gene: 186476398

Centisome position: 53.48

GC content: 66.04

Gene sequence:

>1443_bases
ATGTCGATGTCAGATACGCCGTCCACGCAAACCGTCCCGTCGAACGGACGGCCGTCGCTGATCGTGACGCTCGTGGCCGC
GGCGTTTTTCATGGAGAACCTCGACGGCACCATCATCGCGACGGCGTTGCCGCAGATGGCGCATTCGTTCGGCGTGCATG
CCGTCGACCTGTCCATCGGCATCACGTCGTATCTGCTGACGCTCGCCGTTTTCATTCCGATCAGCGGCTGGGCCGCCGAT
CGCTTCGGCGTGCGCAACGTGTTCACGGCGGCGCTGGCGGTGTTCACGCTGGCGTCGGTGGCGTGCGGGATGACGGACGG
GCTCGTCGAGTTCACCGCGGCGCGCGTGGTGCAAGGCATCGGCGGCGCGATGATGGTGCCCGTCGGCCGCCTTGCCGTGC
TGCGCGCGACGCCCAAGGATGATCTGATGCGCGCGATCTCGATCATCACGTGGCCGGGACTCGTCGCACCCGTCATCGGA
CCGCCGCTCGGCGGCTTTATCACGACGTATTCGTCGTGGCGCTGGATCTTCTATCTGAACGTTCCCCTCGGCATCGTCGG
CATGGTGCTCGCGTGGCGCTTCATCCGTGCCGACCGCGACACGCAGAAGCGTCCGTTCGATACGCTGGGTTTCGCGCTGT
GCGGCGTCGCGGGCACGGCCATCATGTATGCGATGGAACTGCTCGGCCGCTCGGACGCGCCGTGGCGCGAAGCGCTCGCG
TTTCTCGCGGGCGGCATCGCGGCGGGTATCGCGGCCTTCTTTCATCTGCGGCGCGATCCGCATCCCGTCGTCGATCTGTC
GGCGTTTCGCGTGAAGACGTTTGCCGTCGCGATGGGCGGCGGCTCGCTGTTTCGCGTGTCGATCAGCGCAGTGCCGTTTC
TGCTGCCGCTGATGTTCCAGCTCGGCTTCGGTATGAACGCGTTTCACTCTGGGCTGCTGACACTCGTCGTGTTTGCGGGC
AACCTCTCGATGAAGCTCGTGACCACGCCCGTGATGCGCCGCTTCGGCTTTCGTCCCGTGCTGCTCGTAAATGGCGCGCT
CGCGGCATTGAGCATCGCGGCGATGAGTCAGCTGACGGCCGCGACGCCGCTCGTGCTGATGGCCGTCGTGCTGTTCGCGA
GTGGCTTGTCGCGCTCGCTGCAATTCACGGCCGTCAATACACTGAGCTTTGCCGACGTGCCGAAGCGGCAGATGAGCGGC
GCGTCGACCCTCTCGAGCACGCTCTCGCAGATGACGATGGGCATGGGTGTCGCCCTCGGCGCGATCGCAATGCGGCTGGC
GGCGTGGCTGCATGGGCATGAGGCGCAGTCGGTGACGCCGGCGGATTTCAGTGTCGCGTTTCTGCTGGTGGCGGCCGTCG
GGCTGGTGGCCATTGTCGACGTGTTCGGTCTTGAGCGCGATGCGGGCGCACATGTGAGCGGGCATCGGCGGGAACGGACC
TGA

Upstream 100 bases:

>100_bases
CCGCGTCAAACGCGTGCGTGGATAGAATGTCGTCTGAAATGAAGCCTGGCTGAAGCAAGCAAGACGTCCGTTTCGATGCG
CTTACAACGTTCGGAGAATC

Downstream 100 bases:

>100_bases
GTTTGCCGTTCTACTTTGGATTGCGAGGTTGTGCCTGACGATGAACATCGAACAGTTTGAATTGCTCGTGACACGTACGA
TGCCGTACGGCAAGTACAAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSMSDTPSTQTVPSNGRPSLIVTLVAAAFFMENLDGTIIATALPQMAHSFGVHAVDLSIGITSYLLTLAVFIPISGWAAD
RFGVRNVFTAALAVFTLASVACGMTDGLVEFTAARVVQGIGGAMMVPVGRLAVLRATPKDDLMRAISIITWPGLVAPVIG
PPLGGFITTYSSWRWIFYLNVPLGIVGMVLAWRFIRADRDTQKRPFDTLGFALCGVAGTAIMYAMELLGRSDAPWREALA
FLAGGIAAGIAAFFHLRRDPHPVVDLSAFRVKTFAVAMGGGSLFRVSISAVPFLLPLMFQLGFGMNAFHSGLLTLVVFAG
NLSMKLVTTPVMRRFGFRPVLLVNGALAALSIAAMSQLTAATPLVLMAVVLFASGLSRSLQFTAVNTLSFADVPKRQMSG
ASTLSSTLSQMTMGMGVALGAIAMRLAAWLHGHEAQSVTPADFSVAFLLVAAVGLVAIVDVFGLERDAGAHVSGHRRERT

Sequences:

>Translated_480_residues
MSMSDTPSTQTVPSNGRPSLIVTLVAAAFFMENLDGTIIATALPQMAHSFGVHAVDLSIGITSYLLTLAVFIPISGWAAD
RFGVRNVFTAALAVFTLASVACGMTDGLVEFTAARVVQGIGGAMMVPVGRLAVLRATPKDDLMRAISIITWPGLVAPVIG
PPLGGFITTYSSWRWIFYLNVPLGIVGMVLAWRFIRADRDTQKRPFDTLGFALCGVAGTAIMYAMELLGRSDAPWREALA
FLAGGIAAGIAAFFHLRRDPHPVVDLSAFRVKTFAVAMGGGSLFRVSISAVPFLLPLMFQLGFGMNAFHSGLLTLVVFAG
NLSMKLVTTPVMRRFGFRPVLLVNGALAALSIAAMSQLTAATPLVLMAVVLFASGLSRSLQFTAVNTLSFADVPKRQMSG
ASTLSSTLSQMTMGMGVALGAIAMRLAAWLHGHEAQSVTPADFSVAFLLVAAVGLVAIVDVFGLERDAGAHVSGHRRERT
>Mature_479_residues
SMSDTPSTQTVPSNGRPSLIVTLVAAAFFMENLDGTIIATALPQMAHSFGVHAVDLSIGITSYLLTLAVFIPISGWAADR
FGVRNVFTAALAVFTLASVACGMTDGLVEFTAARVVQGIGGAMMVPVGRLAVLRATPKDDLMRAISIITWPGLVAPVIGP
PLGGFITTYSSWRWIFYLNVPLGIVGMVLAWRFIRADRDTQKRPFDTLGFALCGVAGTAIMYAMELLGRSDAPWREALAF
LAGGIAAGIAAFFHLRRDPHPVVDLSAFRVKTFAVAMGGGSLFRVSISAVPFLLPLMFQLGFGMNAFHSGLLTLVVFAGN
LSMKLVTTPVMRRFGFRPVLLVNGALAALSIAAMSQLTAATPLVLMAVVLFASGLSRSLQFTAVNTLSFADVPKRQMSGA
STLSSTLSQMTMGMGVALGAIAMRLAAWLHGHEAQSVTPADFSVAFLLVAAVGLVAIVDVFGLERDAGAHVSGHRRERT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=35.8241758241758, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788392, Length=464, Percent_Identity=34.4827586206897, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788710, Length=457, Percent_Identity=25.8205689277899, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789042, Length=449, Percent_Identity=26.2806236080178, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87081983, Length=287, Percent_Identity=26.1324041811847, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=30.188679245283, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=401, Percent_Identity=23.4413965087282, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=27.6315789473684, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=142, Percent_Identity=28.169014084507, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50724; Mature: 50593

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSDTPSTQTVPSNGRPSLIVTLVAAAFFMENLDGTIIATALPQMAHSFGVHAVDLSIG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEHHHH
ITSYLLTLAVFIPISGWAADRFGVRNVFTAALAVFTLASVACGMTDGLVEFTAARVVQGI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGAMMVPVGRLAVLRATPKDDLMRAISIITWPGLVAPVIGPPLGGFITTYSSWRWIFYLN
CCEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCEEEEEEE
VPLGIVGMVLAWRFIRADRDTQKRPFDTLGFALCGVAGTAIMYAMELLGRSDAPWREALA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FLAGGIAAGIAAFFHLRRDPHPVVDLSAFRVKTFAVAMGGGSLFRVSISAVPFLLPLMFQ
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEEEEECCCCEEEEEHHHHHHHHHHHHH
LGFGMNAFHSGLLTLVVFAGNLSMKLVTTPVMRRFGFRPVLLVNGALAALSIAAMSQLTA
HCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHH
ATPLVLMAVVLFASGLSRSLQFTAVNTLSFADVPKRQMSGASTLSSTLSQMTMGMGVALG
HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
AIAMRLAAWLHGHEAQSVTPADFSVAFLLVAAVGLVAIVDVFGLERDAGAHVSGHRRERT
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SMSDTPSTQTVPSNGRPSLIVTLVAAAFFMENLDGTIIATALPQMAHSFGVHAVDLSIG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEHHHH
ITSYLLTLAVFIPISGWAADRFGVRNVFTAALAVFTLASVACGMTDGLVEFTAARVVQGI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GGAMMVPVGRLAVLRATPKDDLMRAISIITWPGLVAPVIGPPLGGFITTYSSWRWIFYLN
CCEEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCEEEEEEE
VPLGIVGMVLAWRFIRADRDTQKRPFDTLGFALCGVAGTAIMYAMELLGRSDAPWREALA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FLAGGIAAGIAAFFHLRRDPHPVVDLSAFRVKTFAVAMGGGSLFRVSISAVPFLLPLMFQ
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEEEEECCCCEEEEEHHHHHHHHHHHHH
LGFGMNAFHSGLLTLVVFAGNLSMKLVTTPVMRRFGFRPVLLVNGALAALSIAAMSQLTA
HCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHH
ATPLVLMAVVLFASGLSRSLQFTAVNTLSFADVPKRQMSGASTLSSTLSQMTMGMGVALG
HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
AIAMRLAAWLHGHEAQSVTPADFSVAFLLVAAVGLVAIVDVFGLERDAGAHVSGHRRERT
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]