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The map label for this gene is cysU [H]

Identifier: 186476386

GI number: 186476386

Start: 1850775

End: 1851671

Strand: Reverse

Name: cysU [H]

Synonym: Bphy_1628

Alternate gene names: 186476386

Gene position: 1851671-1850775 (Counterclockwise)

Preceding gene: 186476387

Following gene: 186476385

Centisome position: 53.22

GC content: 65.22

Gene sequence:

>897_bases
ATGACGACGTTGACCTTTCGCAAGCCGAGCGCGATACCCGGTTTCGGCGTGACGCTCGGCATCACCGTGGCGTATCTGAG
CCTCGTGGTGCTGATTCCGCTCGCAGCCACTTTCCTCAAGACGGCGACCCTCGACTGGAGCCAGTTCGTGCGCGCCGTCA
CGTCGCCGCGTGTGCTGGCGTCGTACCGGCTGACGTTCTTCGCGGCACTCGGCGGCGCGTTGATCAATGCGGTGTTCGGC
TTTCTGGTCGCGTGGGTGCTGGTGCGCTATCGCTTCCCGTTCAAGCGGATCGTCGATGCCGTCGTCGATCTGCCGTTCGC
GCTGCCGACTTCCGTGGCGGGCATTTCGCTCGCGGCGATCTATTCAGGCAACGGCTGGATCGGCCAGTTTCTCGAACCGC
TCGGCATCAAGATCGCCTTCACTCCGGCGGGCGTGCTGGTCGCGCTGACCTTTATCGGCTTGCCGTTCGTCGTGCGGACG
GTGCAGCCCGTGCTCGAGGAGTTCGAGCGCGAGCAGGAAGAGGCGGCCGCGTGCCTCGGCGCGTCGCGCTGGCTGACATT
CCGCCGCGTCGTTCTGCCTGCCGTGTTTCCCGCGCTGCTGACGGGCTTCGCGCTGGCGTTCGCGCGCGCGCTGGGTGAAT
ATGGCTCGGTGATCTTCATCGCCGGCAATGTGCCGATGAAGTCCGAGATCACGTCGCTGCTCATCATCACGAAGCTCGAG
CAATACGACTATGCGGGCGCAACGGCGCTCGCCGTGGTGATGCTGGTGGTGTCGTTCGTGATGCTGCTGTTGATCAACAC
GCTGCAGTGGTATCTGCAGCGGCGCACGAGCCGCGGGCGCACCGCGCCCACTGTTGCGCCTTCGACAGCCGTCGCCGCAA
CGGGAGGTGTGCAGTGA

Upstream 100 bases:

>100_bases
AGTGACGTCGAACCGCGGCGAAGTTTGAGCAAGGCGCGCCCTCGGGCGCGCTTTCGGCAATGTGCCTATGGCACATGCCT
AACCTGAAAGAGCCTTTCGC

Downstream 100 bases:

>100_bases
GCCAGGATTCCGCTGTGCTGTCGCGTCCGGCGCCGAATAACGCGAATGCCGCGCGCCGTCCCGATCCCGTCACCGAGCCG
CGTGCTGTCCGCTGGGTCCT

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTTLTFRKPSAIPGFGVTLGITVAYLSLVVLIPLAATFLKTATLDWSQFVRAVTSPRVLASYRLTFFAALGGALINAVFG
FLVAWVLVRYRFPFKRIVDAVVDLPFALPTSVAGISLAAIYSGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFVMLLLINTLQWYLQRRTSRGRTAPTVAPSTAVAATGGVQ

Sequences:

>Translated_298_residues
MTTLTFRKPSAIPGFGVTLGITVAYLSLVVLIPLAATFLKTATLDWSQFVRAVTSPRVLASYRLTFFAALGGALINAVFG
FLVAWVLVRYRFPFKRIVDAVVDLPFALPTSVAGISLAAIYSGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFVMLLLINTLQWYLQRRTSRGRTAPTVAPSTAVAATGGVQ
>Mature_297_residues
TTLTFRKPSAIPGFGVTLGITVAYLSLVVLIPLAATFLKTATLDWSQFVRAVTSPRVLASYRLTFFAALGGALINAVFGF
LVAWVLVRYRFPFKRIVDAVVDLPFALPTSVAGISLAAIYSGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRTV
QPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLEQ
YDYAGATALAVVMLVVSFVMLLLINTLQWYLQRRTSRGRTAPTVAPSTAVAATGGVQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=49.2424242424242, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87082099, Length=264, Percent_Identity=33.7121212121212, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=33.6633663366337, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786254, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787369, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787368, Length=241, Percent_Identity=29.045643153527, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32084; Mature: 31953

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTFRKPSAIPGFGVTLGITVAYLSLVVLIPLAATFLKTATLDWSQFVRAVTSPRVLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
SYRLTFFAALGGALINAVFGFLVAWVLVRYRFPFKRIVDAVVDLPFALPTSVAGISLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHEEEEE
YSGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
ECCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFVMLLLINTLQWYLQRRTSRGRTAPTVAPSTAVAATGGVQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TTLTFRKPSAIPGFGVTLGITVAYLSLVVLIPLAATFLKTATLDWSQFVRAVTSPRVLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
SYRLTFFAALGGALINAVFGFLVAWVLVRYRFPFKRIVDAVVDLPFALPTSVAGISLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHEEEEE
YSGNGWIGQFLEPLGIKIAFTPAGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
ECCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFVMLLLINTLQWYLQRRTSRGRTAPTVAPSTAVAATGGVQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]