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The map label for this gene is cysW [H]

Identifier: 186476385

GI number: 186476385

Start: 1849774

End: 1850778

Strand: Reverse

Name: cysW [H]

Synonym: Bphy_1627

Alternate gene names: 186476385

Gene position: 1850778-1849774 (Counterclockwise)

Preceding gene: 186476386

Following gene: 186476384

Centisome position: 53.2

GC content: 63.98

Gene sequence:

>1005_bases
GTGAGCCAGGATTCCGCTGTGCTGTCGCGTCCGGCGCCGAATAACGCGAATGCCGCGCGCCGTCCCGATCCCGTCACCGA
GCCGCGTGCTGTCCGCTGGGTCCTGACGATCATCGCGTTGCTGTTCCTCGCGCTGTTTCTGGTGCTGCCGCTCGTCGCCG
TGTTCTATCAGGCGTTGAGCAAGGGCATTGCGTTCTACTTCGAGTCGCTTGCCGATCCCGATGCGGTGTCCGCGATCAAG
CTCACGCTGCTGACGGCCGCGATCGCCGTGCCGCTCAATCTCGTGTTCGGACTCGCGGCGTCGTGGTGTATCGCGAAGTT
CGAGTTTCGCGGCAAGGCCTTGCTGACGACGCTGATCGACTTGCCGTTTTCGGTGTCGCCCGTGATCTCCGGCCTGATCT
ACGTGCTGATGTTCGGCGCGCAGGGCTGGTTCGGGCCGTGGCTTGCCGATCACAACGTGCAGATCATCTTCGCGGTGCCG
GGTATCGTGCTCGCGACCATCTTCGTCACGTTCCCGTTCGTTGCCCGAGAACTGATTCCGCTGATGCAGGCGCAAGGCAA
TGACGAGGAAGAAGCCGCGCACGTGCTCGGCGCATCGGGCTGGCAGATCTTCCGGCGCGTCACGCTGCCGAACGTCAAGT
GGGGTCTGCTGTATGGCGTGATTCTCTGCAACGCGCGTGCGATGGGCGAGTTCGGCGCGGTGTCGGTGGTGTCGGGCCAC
ATTCGCGGGCAGACGGACACGATGCCCCTGCACGTCGAGATTCTCTACAACGAATATAACTTTTCGGCGGCCTTCGCCGT
GGCGTCGGTGCTCGCGTTGCTGGCACTCGTCACGCTCGGCCTGAAGCTGCTGGCCGAGCGCCACATGTCGGCGGAACTGG
CGAGTGCGAACGATGTGCCTGCGCATGCCGGTCCCGGTTCACATTCCGCTGCACATTCCGCTGCACAGCCGGCCGCACAT
TCGCCTGAGGGTGCGACCCAGCACGCGGCACAACCGGTTCAGTAA

Upstream 100 bases:

>100_bases
TCAACACGCTGCAGTGGTATCTGCAGCGGCGCACGAGCCGCGGGCGCACCGCGCCCACTGTTGCGCCTTCGACAGCCGTC
GCCGCAACGGGAGGTGTGCA

Downstream 100 bases:

>100_bases
GGAGAGTTGTCAAAGATGGGCATTACCGTACGCAATCTGCAAAAACGCTTTGGCGACTTCACCGCCCTCGACAACGTCTC
GCTCGATTTTCCACCGGGCG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSQDSAVLSRPAPNNANAARRPDPVTEPRAVRWVLTIIALLFLALFLVLPLVAVFYQALSKGIAFYFESLADPDAVSAIK
LTLLTAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLADHNVQIIFAVP
GIVLATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGH
IRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELASANDVPAHAGPGSHSAAHSAAQPAAH
SPEGATQHAAQPVQ

Sequences:

>Translated_334_residues
MSQDSAVLSRPAPNNANAARRPDPVTEPRAVRWVLTIIALLFLALFLVLPLVAVFYQALSKGIAFYFESLADPDAVSAIK
LTLLTAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLADHNVQIIFAVP
GIVLATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGH
IRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELASANDVPAHAGPGSHSAAHSAAQPAAH
SPEGATQHAAQPVQ
>Mature_333_residues
SQDSAVLSRPAPNNANAARRPDPVTEPRAVRWVLTIIALLFLALFLVLPLVAVFYQALSKGIAFYFESLADPDAVSAIKL
TLLTAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLADHNVQIIFAVPG
IVLATIFVTFPFVARELIPLMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGHI
RGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELASANDVPAHAGPGSHSAAHSAAQPAAHS
PEGATQHAAQPVQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=271, Percent_Identity=52.3985239852399, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=38.3495145631068, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=34.2541436464088, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35621; Mature: 35490

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDSAVLSRPAPNNANAARRPDPVTEPRAVRWVLTIIALLFLALFLVLPLVAVFYQALS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIAFYFESLADPDAVSAIKLTLLTAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LPFSVSPVISGLIYVLMFGAQGWFGPWLADHNVQIIFAVPGIVLATIFVTFPFVARELIP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
LMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGH
HHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHCCC
IRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELASANDVP
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AHAGPGSHSAAHSAAQPAAHSPEGATQHAAQPVQ
CCCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure 
SQDSAVLSRPAPNNANAARRPDPVTEPRAVRWVLTIIALLFLALFLVLPLVAVFYQALS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIAFYFESLADPDAVSAIKLTLLTAAIAVPLNLVFGLAASWCIAKFEFRGKALLTTLID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LPFSVSPVISGLIYVLMFGAQGWFGPWLADHNVQIIFAVPGIVLATIFVTFPFVARELIP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
LMQAQGNDEEEAAHVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSGH
HHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHCCC
IRGQTDTMPLHVEILYNEYNFSAAFAVASVLALLALVTLGLKLLAERHMSAELASANDVP
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AHAGPGSHSAAHSAAQPAAHSPEGATQHAAQPVQ
CCCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]