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The map label for this gene is dmlR [H]

Identifier: 186476377

GI number: 186476377

Start: 1839948

End: 1840859

Strand: Reverse

Name: dmlR [H]

Synonym: Bphy_1619

Alternate gene names: 186476377

Gene position: 1840859-1839948 (Counterclockwise)

Preceding gene: 186476381

Following gene: 186476374

Centisome position: 52.91

GC content: 64.04

Gene sequence:

>912_bases
ATGGACCGCTTCAAGCAGATCGAAACGTTCGTGCGCGTCGCCGACGCGGGCAGCCTTGCGGCGGCGGCGCTCGAAGAGGG
CGTGTCGCCCGTGATCCTCGGGCGCCGGATCGACGCACTCGAAAAGCGTCTCGGCGTCAAGCTGATGTACCGCTCGACGC
GGCGCCTCGTCGTGAGCGAAGAAGGCGCGGCGTTTCTCGAACGCTGCCGCGGGCTGCTCAGTGAATGGGATCAGGCGGAA
AACGAATTGATGGCGGGACGGCGCTCGGTGAGCGGACATCTGATCGTGTCGGCGCCCGCCGCATTCGGGCGCAAGCACGT
CGCGCCGCTCGCGCCGGAGTTCCTGAAGGACAAGCCGGAACTGCAGGTGTCGTTCAATTTGACCGACCGCGTGGTCGATC
TGGTGCGCGAGGGCTACGACCTGTCGATCCGGATCGGCGGCGCTGTCGATCCGAACTTCGTCGCGGTGAAGCTCGCGACC
AACCGGCGCGTGGTGTGCGGCACCCCCGAATATTTCCGCAAGCACGGCAAGCCGAAGACGCTCGAAGACCTGCCAAATCA
CAACTGCTTCGCGTTCAACCTGCAAGGCGGGCAGAACCGCGGCTGGTATTTCCGCCGCAACGGCAAGCTGACGACGGTGC
GCGTAAGCGGCACGCTCGATTGCAACGACGGCGAACTGCTGCACCGGTGGGTGTCGGAGGGGCTCGGGCTCGGCTGGCGC
TCGACCTGGGAAATCGATCAGCAGCTCGTGCGCGGCGAGCTCGAAACGGTCCTCGACGAATACGCGCTGCCCGACTACGA
CATCCTCGCCGTGTATCCGCAGCAGCGCTATGTGCCCGCGAAAGTGCGCTATTTCATCGATTATCTGAAAGAGGTCTATG
CGCGAAAGGATTATTGGGCGAATGCGTCATGA

Upstream 100 bases:

>100_bases
CGCTTATTTCAAAACAAAAAGTATGGAATACGCGCGCCGCGCGGCTTTGCACGCCGCCGCCGGCAGCGACGGCAAGCCGC
TACAGGCCGAGGAGACAAGG

Downstream 100 bases:

>100_bases
GCGGTTGAATCAGCCCACGACGATCGTCCCGTTCATCGTCTCGTGCCCCGACCCGCAGAAGATGTCGCACAGAAAATCGA
CGGTGCCCGCTGCACCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELMAGRRSVSGHLIVSAPAAFGRKHVAPLAPEFLKDKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAT
NRRVVCGTPEYFRKHGKPKTLEDLPNHNCFAFNLQGGQNRGWYFRRNGKLTTVRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIDQQLVRGELETVLDEYALPDYDILAVYPQQRYVPAKVRYFIDYLKEVYARKDYWANAS

Sequences:

>Translated_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELMAGRRSVSGHLIVSAPAAFGRKHVAPLAPEFLKDKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAT
NRRVVCGTPEYFRKHGKPKTLEDLPNHNCFAFNLQGGQNRGWYFRRNGKLTTVRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIDQQLVRGELETVLDEYALPDYDILAVYPQQRYVPAKVRYFIDYLKEVYARKDYWANAS
>Mature_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELMAGRRSVSGHLIVSAPAAFGRKHVAPLAPEFLKDKPELQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAT
NRRVVCGTPEYFRKHGKPKTLEDLPNHNCFAFNLQGGQNRGWYFRRNGKLTTVRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIDQQLVRGELETVLDEYALPDYDILAVYPQQRYVPAKVRYFIDYLKEVYARKDYWANAS

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=36.6666666666667, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=32.6315789473684, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=31.5972222222222, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=27.6450511945392, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.962457337884, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.6223776223776, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1789173, Length=204, Percent_Identity=28.921568627451, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34352; Mature: 34352

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELMAGRRSVSGHLIVSAPAAFGRKHVAPLAPEFLKDKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLATNRRVVCGTPEYFRKHGKPKT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LEDLPNHNCFAFNLQGGQNRGWYFRRNGKLTTVRVSGTLDCNDGELLHRWVSEGLGLGWR
HHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCC
STWEIDQQLVRGELETVLDEYALPDYDILAVYPQQRYVPAKVRYFIDYLKEVYARKDYWA
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NAS
CCC
>Mature Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVILGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELMAGRRSVSGHLIVSAPAAFGRKHVAPLAPEFLKDKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQVSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLATNRRVVCGTPEYFRKHGKPKT
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LEDLPNHNCFAFNLQGGQNRGWYFRRNGKLTTVRVSGTLDCNDGELLHRWVSEGLGLGWR
HHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCC
STWEIDQQLVRGELETVLDEYALPDYDILAVYPQQRYVPAKVRYFIDYLKEVYARKDYWA
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NAS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]